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Summary[edit | edit source]

  • pan ID?: SAUPAN000382000
  • symbol?:
  • synonym:
  • description?: tRNA-dihydrouridine synthase

      descriptions from strain specific annotations:

    • tRNA-dihydrouridine synthase
    • putative tRNA-dihydrouridine synthase
    • dihydrouridine synthase (Dus) family protein
    • nitrogen regulation protein NIFR3
    • tRNA dihydrouridine synthase B
  • strand?: -
  • coordinates?: 391879..392865
  • synteny block?: BlockID0001730
  • occurrence?: in 100% of 34 strains

dusB2 : tRNA-dihydrouridine synthase [1]

Certain staphylococcal tRNA sequences require reduction of uridine (U) nucleobases to dihydrouridines (D) to impart more flexibility and reduced aromatic stacking interactions. DusB2 is able to reduce uridines at positions D17, D20 and/or D20a, as appropriate. A clear listing of which tRNAs require such modifications in staphylococci is not available, although it is available for Bacillus and many staphylococcal tRNAs are the same sequence as their Bacillus orthologues. Some staphylococci also contain a partial duplication of dusB2 which is not thought to be functional.

Orthologs[edit | edit source]

    COL:
    N315:
    NCTC8325:
    Newman:
    USA300_FPR3757:
    04-02981:
    SA2981_0089
    08BA02176:
    C248_0078
    11819-97:
    MS7_0083 (dus)
    6850:
    RSAU_000036 (dus)
    71193:
    ST398NM01_0103
    ECT-R 2:
    ECTR2_47
    ED133:
    SAOV_0041
    ED98:
    SAAV_0037
    HO 5096 0412:
    SAEMRSA15_00530
    JH1:
    SaurJH1_0079
    JH9:
    SaurJH9_0077
    JKD6008:
    SAA6008_00075 (dus)
    JKD6159:
    SAA6159_00068 (dus)
    JSNZ:
    JSNZ_000034
    LGA251:
    SARLGA251_00690
    M013:
    M013TW_0076
    MRSA252:
    SAR0099
    MSHR1132:
    SAMSHR1132_00650
    MSSA476:
    SAS0059
    Mu3:
    SAHV_0088
    Mu50:
    SAV0089
    MW2:
    MW0059
    RF122:
    SAB0033c
    ST398:
    SAPIG0103
    T0131:
    SAT0131_00075
    TCH60:
    HMPREF0772_10401
    TW20:
    SATW20_01080
    USA300_TCH1516:
    USA300HOU_0096
    VC40:
    SAVC_00170

Genome Viewer[edit | edit source]

COL
N315
NCTC8325
Newman
USA300_FPR3757

Alignments[edit | edit source]

  • alignment of orthologues:
    CLUSTAL format alignment by MAFFT L-INS-i (v7.307)


    COL             ------------------MEDVTDIVFRHVVSEAARPDVFFTEFTNTESFCHPEGIHSVR
    N315            MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEFTNTESFCHPEGIHSVR
    NCTC8325        ------------------MEDVTDIVFRHVVSEAARPDVFFTEFTNTESFCHPEGIHSVR
    Newman          MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEFTNTESFCHPEGIHSVR
    USA300_FPR3757  ------------------MEDVTDIVFRHVVSEAARPDVFFTEFTNTESFCHPEGIHSVR
                                      ******************************************

    COL             GRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLNMGCPVANVAKKGKGSGLI
    N315            GRLTFSEDEHPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLNMGCPVANVAKKGKGSGLI
    NCTC8325        GRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLNMGCPVANVAKKGKGSGLI
    Newman          GRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLNMGCPVANVAKKGKGSGLI
    USA300_FPR3757  GRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLNMGCPVANVAKKGKGSGLI
                    *********:**************************************************

    COL             LRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKHVFEQDIANLSIHLRTRKEMS
    N315            LRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKHVFEQDIANLSIHLRTRKEMS
    NCTC8325        LRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKHVFEQDIANLSIHLRTRKEMS
    Newman          LRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKHVFEQDIANLSIHLRTRKEMS
    USA300_FPR3757  LRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKHVFEQDIANLSIHLRTRKEMS
                    ************************************************************

    COL             KVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFHNPF
    N315            KVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFHNPF
    NCTC8325        KVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFHNPF
    Newman          KVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFHNPF
    USA300_FPR3757  KVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFHNPF
                    ************************************************************

    COL             AFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLRRFFKIYVRGIRGASELRHQL
    N315            AFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLRRFFKIYVRGIRGASELRHQL
    NCTC8325        AFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLRRFFKIYVRGIRGASELRHQL
    Newman          AFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLRRFFKIYVRGIRGASELRHQL
    USA300_FPR3757  AFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLRRFFKIYVRGIRGASELRHQL
                    ************************************************************

    COL             MNTQSIAEARALLDEFEAQMDEDVKIEL
    N315            MNTQSIAEARALLDEFEAQMDEDVKIEL
    NCTC8325        MNTQSIAEARALLDEFEAQMDEDVKIEL
    Newman          MNTQSIAEARALLDEFEAQMDEDVKIEL
    USA300_FPR3757  MNTQSIAEARALLDEFEAQMDEDVKIEL
                    ****************************

  1. Laura Antoine, Philippe Wolff, Eric Westhof, Pascale Romby, Stefano Marzi
    Mapping post-transcriptional modifications in Staphylococcus aureus tRNAs by nanoLC/MSMS.
    Biochimie: 2019, 164;60-69
    [PubMed:31295507] [WorldCat.org] [DOI] (I p)
    Bruno Faivre, Murielle Lombard, Soufyan Fakroun, Chau-Duy-Tam Vo, Catherine Goyenvalle, Vincent Guérineau, Ludovic Pecqueur, Marc Fontecave, Valérie De Crécy-Lagard, Damien Brégeon, Djemel Hamdane
    Dihydrouridine synthesis in tRNAs is under reductive evolution in Mollicutes.
    RNA Biol: 2021, 18(12);2278-2289
    [PubMed:33685366] [WorldCat.org] [DOI] (I p)
    Claudia Sudol, Lea-Marie Kilz, Virginie Marchand, Quentin Thullier, Vincent Guérineau, Catherine Goyenvalle, Bruno Faivre, Sabrine Toubdji, Murielle Lombard, Olivier Jean-Jean, Valérie de Crécy-Lagard, Mark Helm, Yuri Motorin, Damien Brégeon, Djemel Hamdane
    Functional redundancy in tRNA dihydrouridylation.
    Nucleic Acids Res: 2024, 52(10);5880-5894
    [PubMed:38682613] [WorldCat.org] [DOI] (I p)