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⊟Summary[edit | edit source]
- pan ID?: SAUPAN000382000
- symbol?: —
- synonym:
- description?: tRNA-dihydrouridine synthase
- tRNA-dihydrouridine synthase
- putative tRNA-dihydrouridine synthase
- dihydrouridine synthase (Dus) family protein
- nitrogen regulation protein NIFR3
- tRNA dihydrouridine synthase B
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 391879..392865
- synteny block?: BlockID0001730
- occurrence?: in 100% of 34 strains
dusB2 : tRNA-dihydrouridine synthase [1]
Certain staphylococcal tRNA sequences require reduction of uridine (U) nucleobases to dihydrouridines (D) to impart more flexibility and reduced aromatic stacking interactions. DusB2 is able to reduce uridines at positions D17, D20 and/or D20a, as appropriate. A clear listing of which tRNAs require such modifications in staphylococci is not available, although it is available for Bacillus and many staphylococcal tRNAs are the same sequence as their Bacillus orthologues. Some staphylococci also contain a partial duplication of dusB2 which is not thought to be functional.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_0089
08BA02176:
C248_0078
11819-97:
MS7_0083 (dus)
6850:
RSAU_000036 (dus)
71193:
ST398NM01_0103
ECT-R 2:
ECTR2_47
ED133:
SAOV_0041
ED98:
SAAV_0037
HO 5096 0412:
SAEMRSA15_00530
JH1:
SaurJH1_0079
JH9:
SaurJH9_0077
JKD6008:
SAA6008_00075 (dus)
JKD6159:
SAA6159_00068 (dus)
JSNZ:
JSNZ_000034
LGA251:
SARLGA251_00690
M013:
M013TW_0076
MRSA252:
SAR0099
MSHR1132:
SAMSHR1132_00650
MSSA476:
SAS0059
Mu3:
SAHV_0088
Mu50:
SAV0089
MW2:
MW0059
RF122:
SAB0033c
ST398:
SAPIG0103
T0131:
SAT0131_00075
TCH60:
HMPREF0772_10401
TW20:
SATW20_01080
USA300_TCH1516:
USA300HOU_0096
VC40:
SAVC_00170
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL ------------------MEDVTDIVFRHVVSEAARPDVFFTEFTNTESFCHPEGIHSVR
N315 MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEFTNTESFCHPEGIHSVR
NCTC8325 ------------------MEDVTDIVFRHVVSEAARPDVFFTEFTNTESFCHPEGIHSVR
Newman MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEFTNTESFCHPEGIHSVR
USA300_FPR3757 ------------------MEDVTDIVFRHVVSEAARPDVFFTEFTNTESFCHPEGIHSVR
******************************************
COL GRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLNMGCPVANVAKKGKGSGLI
N315 GRLTFSEDEHPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLNMGCPVANVAKKGKGSGLI
NCTC8325 GRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLNMGCPVANVAKKGKGSGLI
Newman GRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLNMGCPVANVAKKGKGSGLI
USA300_FPR3757 GRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLNMGCPVANVAKKGKGSGLI
*********:**************************************************
COL LRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKHVFEQDIANLSIHLRTRKEMS
N315 LRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKHVFEQDIANLSIHLRTRKEMS
NCTC8325 LRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKHVFEQDIANLSIHLRTRKEMS
Newman LRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKHVFEQDIANLSIHLRTRKEMS
USA300_FPR3757 LRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKHVFEQDIANLSIHLRTRKEMS
************************************************************
COL KVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFHNPF
N315 KVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFHNPF
NCTC8325 KVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFHNPF
Newman KVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFHNPF
USA300_FPR3757 KVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFHNPF
************************************************************
COL AFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLRRFFKIYVRGIRGASELRHQL
N315 AFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLRRFFKIYVRGIRGASELRHQL
NCTC8325 AFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLRRFFKIYVRGIRGASELRHQL
Newman AFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLRRFFKIYVRGIRGASELRHQL
USA300_FPR3757 AFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLRRFFKIYVRGIRGASELRHQL
************************************************************
COL MNTQSIAEARALLDEFEAQMDEDVKIEL
N315 MNTQSIAEARALLDEFEAQMDEDVKIEL
NCTC8325 MNTQSIAEARALLDEFEAQMDEDVKIEL
Newman MNTQSIAEARALLDEFEAQMDEDVKIEL
USA300_FPR3757 MNTQSIAEARALLDEFEAQMDEDVKIEL
****************************
- ↑ Laura Antoine, Philippe Wolff, Eric Westhof, Pascale Romby, Stefano Marzi
Mapping post-transcriptional modifications in Staphylococcus aureus tRNAs by nanoLC/MSMS.
Biochimie: 2019, 164;60-69
[PubMed:31295507] [WorldCat.org] [DOI] (I p)Bruno Faivre, Murielle Lombard, Soufyan Fakroun, Chau-Duy-Tam Vo, Catherine Goyenvalle, Vincent Guérineau, Ludovic Pecqueur, Marc Fontecave, Valérie De Crécy-Lagard, Damien Brégeon, Djemel Hamdane
Dihydrouridine synthesis in tRNAs is under reductive evolution in Mollicutes.
RNA Biol: 2021, 18(12);2278-2289
[PubMed:33685366] [WorldCat.org] [DOI] (I p)Claudia Sudol, Lea-Marie Kilz, Virginie Marchand, Quentin Thullier, Vincent Guérineau, Catherine Goyenvalle, Bruno Faivre, Sabrine Toubdji, Murielle Lombard, Olivier Jean-Jean, Valérie de Crécy-Lagard, Mark Helm, Yuri Motorin, Damien Brégeon, Djemel Hamdane
Functional redundancy in tRNA dihydrouridylation.
Nucleic Acids Res: 2024, 52(10);5880-5894
[PubMed:38682613] [WorldCat.org] [DOI] (I p)