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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0318 [new locus tag: SACOL_RS01605 ]
  • pan locus tag?: SAUPAN001246000
  • symbol: int
  • pan gene symbol?: int1
  • synonym:
  • product: prophage L54a, integrase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0318 [new locus tag: SACOL_RS01605 ]
  • symbol: int
  • product: prophage L54a, integrase
  • replicon: chromosome
  • strand: -
  • coordinates: 354785..355990
  • length: 1206
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTGGTTTGAAAAATTTAAAAATAAGAACAATGAAACGAAGTATAGATACTACGAGAAA
    TACAAAGATCCGTATACAGATAAATGGAAACGTGTAAGTGTTGTCTTGAATAAGAATACA
    AAGCAATCGCAAAAAGAGGCAATGTTTCGTTTAGAAGAAAAAATAAAAGAAAAACTAAAC
    AACAAGTCGTCAAGCGAATTAAAAACTTTGACTTTTCACGCGTTATTAGATGAATGGCTT
    GAATATCATATAAAAACATCTGGCTTTAAAGTAACGACGCTTGATAATTTGAAAACAAGA
    ATCAAAAACATCAAAAAGAACAGTTCTCAAAATTTACTTTTAAACAAAATTGATACAAAG
    TACATGCAAACATTTATTAACGAATTATCAAACGTATATTCTGCAAATCAGGTAAAGCGT
    CAACTTGGACATATGAAAGAAGCTATTAAATACGCCGTTAAATTTTACAATTATCCAAAC
    GAACACATATTAAATAGCGTCACACTACCAAAGAAGAGTAAGACGATAGAAGATATAGAA
    AAAGAAGAAGCGAAAATGTACAACTATTTAGAGATGGAACAGGTAATACAGATACGCGAT
    TTTATACTGAACGATAATAACATGCAGTATAGAGCTCGTATTTTAGTTGCTGGGGCTGTA
    GAAGTTCAAGCTTTAACAGGTATGCGCATAGGTGAGTTATTAGCTCTCCAAGTTAAAGAT
    GTTGACCTCAAAAATAAAACGATCGATATTAATGGCACTATTCACAGAATCAAATGTAAT
    GCTGGATTTGGTCACAAAGATACTACGAAGACCGCAGGTTCAAAAAGAAAAATCGCCATC
    AATTCAAGGATAGCAAATGTATTGAAAAAAATAATGTTAGAAAATAAAAAGATGCAACAA
    TGGGAACCAAGCTATGTTGATAGAGGGTTTATATTCACAACTTGCCAAGGAAATCCTATG
    CAAGGCAGTAGGATAAACAAACGATTGTCCTCAGCTGCAGAATCATTAAATATAAATAAA
    AAAGTTACTACTCACACACTAAGGCATACACACATAAGTTTATTGGCGGAAATGAATATA
    TCGTTAAAAGCAATTATGAAAAGAGTAGGACATACAGATGAAAAAACGACTATAAAGGTA
    TATACCCATGTAACAGAGAAAATGGACAGAGAGTTAGAGCAAAAATTAGAAAAACTTGTG
    TACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1206

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0318 [new locus tag: SACOL_RS01605 ]
  • symbol: Int
  • description: prophage L54a, integrase
  • length: 401
  • theoretical pI: 10.3392
  • theoretical MW: 46973.1
  • GRAVY: -0.697756

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 73.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 71.5)
    and 2 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 54.9)
    Genetic information processing Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 54.9)
  • TheSEED  :
    • Phage integrase
  • PFAM:
    DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 99.5)
    and 3 more
    l-integrase_N (CL0469) Phage_int_SAM_3; Phage integrase, N-terminal SAM-like domain (PF14659; HMM-score: 23.5)
    HIT (CL0265) DcpS_C; Scavenger mRNA decapping enzyme C-term binding (PF11969; HMM-score: 15.8)
    l-integrase_N (CL0469) Phage_int_SAM_1; Phage integrase, N-terminal SAM-like domain (PF02899; HMM-score: 14.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.01108
    • TAT(Tat/SPI): 0.000421
    • LIPO(Sec/SPII): 0.003012
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MWFEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQKEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGFKVTTLDNLKTRIKNIKKNSSQNLLLNKIDTKYMQTFINELSNVYSANQVKRQLGHMKEAIKYAVKFYNYPNEHILNSVTLPKKSKTIEDIEKEEAKMYNYLEMEQVIQIRDFILNDNNMQYRARILVAGAVEVQALTGMRIGELLALQVKDVDLKNKTIDINGTIHRIKCNAGFGHKDTTKTAGSKRKIAINSRIANVLKKIMLENKKMQQWEPSYVDRGFIFTTCQGNPMQGSRINKRLSSAAESLNINKKVTTHTLRHTHISLLAEMNISLKAIMKRVGHTDEKTTIKVYTHVTEKMDRELEQKLEKLVY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

This is the integrase for prophage ΦCOL6. It is a site-specific class Sa6int integrase that recognizes the TGGCAAGTT-n76-AACTTGCCA attB sequence and catalyzes either integration or excision of a prophage, typically via duplication of n7-n24. The attB sequence for Sa6int prophage in Staphylococcus aureus is found almost exclusively near the end of the coding sequence for the long-chain triacylglycerol ester hydrolase (gehB), sometimes annotated more simply as lipase (lip).

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Christiane Goerke, Roman Pantucek, Silva Holtfreter, Berit Schulte, Manuel Zink, Dorothee Grumann, Barbara M Bröker, Jiri Doskar, Christiane Wolz
    Diversity of prophages in dominant Staphylococcus aureus clonal lineages.
    J Bacteriol: 2009, 191(11);3462-8
    [PubMed:19329640] [WorldCat.org] [DOI] (I p)
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