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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS01605 [old locus tag: SACOL0318 ]
  • pan locus tag?: SAUPAN001246000
  • symbol: SACOL_RS01605
  • pan gene symbol?: int1
  • synonym:
  • product: site-specific integrase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS01605 [old locus tag: SACOL0318 ]
  • symbol: SACOL_RS01605
  • product: site-specific integrase
  • replicon: chromosome
  • strand: -
  • coordinates: 354785..355990
  • length: 1206
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (354785..355990) NCBI
  • BioCyc: SACOL_RS01605 BioCyc
  • MicrobesOnline: see SACOL0318

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTGGTTTGAAAAATTTAAAAATAAGAACAATGAAACGAAGTATAGATACTACGAGAAA
    TACAAAGATCCGTATACAGATAAATGGAAACGTGTAAGTGTTGTCTTGAATAAGAATACA
    AAGCAATCGCAAAAAGAGGCAATGTTTCGTTTAGAAGAAAAAATAAAAGAAAAACTAAAC
    AACAAGTCGTCAAGCGAATTAAAAACTTTGACTTTTCACGCGTTATTAGATGAATGGCTT
    GAATATCATATAAAAACATCTGGCTTTAAAGTAACGACGCTTGATAATTTGAAAACAAGA
    ATCAAAAACATCAAAAAGAACAGTTCTCAAAATTTACTTTTAAACAAAATTGATACAAAG
    TACATGCAAACATTTATTAACGAATTATCAAACGTATATTCTGCAAATCAGGTAAAGCGT
    CAACTTGGACATATGAAAGAAGCTATTAAATACGCCGTTAAATTTTACAATTATCCAAAC
    GAACACATATTAAATAGCGTCACACTACCAAAGAAGAGTAAGACGATAGAAGATATAGAA
    AAAGAAGAAGCGAAAATGTACAACTATTTAGAGATGGAACAGGTAATACAGATACGCGAT
    TTTATACTGAACGATAATAACATGCAGTATAGAGCTCGTATTTTAGTTGCTGGGGCTGTA
    GAAGTTCAAGCTTTAACAGGTATGCGCATAGGTGAGTTATTAGCTCTCCAAGTTAAAGAT
    GTTGACCTCAAAAATAAAACGATCGATATTAATGGCACTATTCACAGAATCAAATGTAAT
    GCTGGATTTGGTCACAAAGATACTACGAAGACCGCAGGTTCAAAAAGAAAAATCGCCATC
    AATTCAAGGATAGCAAATGTATTGAAAAAAATAATGTTAGAAAATAAAAAGATGCAACAA
    TGGGAACCAAGCTATGTTGATAGAGGGTTTATATTCACAACTTGCCAAGGAAATCCTATG
    CAAGGCAGTAGGATAAACAAACGATTGTCCTCAGCTGCAGAATCATTAAATATAAATAAA
    AAAGTTACTACTCACACACTAAGGCATACACACATAAGTTTATTGGCGGAAATGAATATA
    TCGTTAAAAGCAATTATGAAAAGAGTAGGACATACAGATGAAAAAACGACTATAAAGGTA
    TATACCCATGTAACAGAGAAAATGGACAGAGAGTTAGAGCAAAAATTAGAAAAACTTGTG
    TACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1206

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS01605 [old locus tag: SACOL0318 ]
  • symbol: SACOL_RS01605
  • description: site-specific integrase
  • length: 401
  • theoretical pI: 10.3392
  • theoretical MW: 46973.1
  • GRAVY: -0.697756

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 73.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 71.5)
    and 2 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 54.9)
    Genetic information processing Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 54.9)
  • TheSEED: see SACOL0318
  • PFAM:
    DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 99.5)
    and 3 more
    l-integrase_N (CL0469) Phage_int_SAM_3; Phage integrase, N-terminal SAM-like domain (PF14659; HMM-score: 23.5)
    HIT (CL0265) DcpS_C; Scavenger mRNA decapping enzyme C-term binding (PF11969; HMM-score: 15.8)
    l-integrase_N (CL0469) Phage_int_SAM_1; Phage integrase, N-terminal SAM-like domain (PF02899; HMM-score: 14.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.01108
    • TAT(Tat/SPI): 0.000421
    • LIPO(Sec/SPII): 0.003012
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MWFEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQKEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGFKVTTLDNLKTRIKNIKKNSSQNLLLNKIDTKYMQTFINELSNVYSANQVKRQLGHMKEAIKYAVKFYNYPNEHILNSVTLPKKSKTIEDIEKEEAKMYNYLEMEQVIQIRDFILNDNNMQYRARILVAGAVEVQALTGMRIGELLALQVKDVDLKNKTIDINGTIHRIKCNAGFGHKDTTKTAGSKRKIAINSRIANVLKKIMLENKKMQQWEPSYVDRGFIFTTCQGNPMQGSRINKRLSSAAESLNINKKVTTHTLRHTHISLLAEMNISLKAIMKRVGHTDEKTTIKVYTHVTEKMDRELEQKLEKLVY

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: see SACOL0318
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]