PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS01605 [old locus tag: SACOL0318 ]
• pan locus tag^?: SAUPAN001246000
• symbol: SACOL_RS01605
• pan gene symbol^?: int1
• synonym:
• product: site-specific integrase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS01605 [old locus tag: SACOL0318 ]
• symbol: SACOL_RS01605
• product: site-specific integrase
• replicon: chromosome
• strand: -
• coordinates: 354785..355990
• length: 1206
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (354785..355990) NCBI
• BioCyc: SACOL_RS01605 BioCyc
• MicrobesOnline: see SACOL0318

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGTGGTTTGAAAAATTTAAAAATAAGAACAATGAAACGAAGTATAGATACTACGAGAAA
  TACAAAGATCCGTATACAGATAAATGGAAACGTGTAAGTGTTGTCTTGAATAAGAATACA
  AAGCAATCGCAAAAAGAGGCAATGTTTCGTTTAGAAGAAAAAATAAAAGAAAAACTAAAC
  AACAAGTCGTCAAGCGAATTAAAAACTTTGACTTTTCACGCGTTATTAGATGAATGGCTT
  GAATATCATATAAAAACATCTGGCTTTAAAGTAACGACGCTTGATAATTTGAAAACAAGA
  ATCAAAAACATCAAAAAGAACAGTTCTCAAAATTTACTTTTAAACAAAATTGATACAAAG
  TACATGCAAACATTTATTAACGAATTATCAAACGTATATTCTGCAAATCAGGTAAAGCGT
  CAACTTGGACATATGAAAGAAGCTATTAAATACGCCGTTAAATTTTACAATTATCCAAAC
  GAACACATATTAAATAGCGTCACACTACCAAAGAAGAGTAAGACGATAGAAGATATAGAA
  AAAGAAGAAGCGAAAATGTACAACTATTTAGAGATGGAACAGGTAATACAGATACGCGAT
  TTTATACTGAACGATAATAACATGCAGTATAGAGCTCGTATTTTAGTTGCTGGGGCTGTA
  GAAGTTCAAGCTTTAACAGGTATGCGCATAGGTGAGTTATTAGCTCTCCAAGTTAAAGAT
  GTTGACCTCAAAAATAAAACGATCGATATTAATGGCACTATTCACAGAATCAAATGTAAT
  GCTGGATTTGGTCACAAAGATACTACGAAGACCGCAGGTTCAAAAAGAAAAATCGCCATC
  AATTCAAGGATAGCAAATGTATTGAAAAAAATAATGTTAGAAAATAAAAAGATGCAACAA
  TGGGAACCAAGCTATGTTGATAGAGGGTTTATATTCACAACTTGCCAAGGAAATCCTATG
  CAAGGCAGTAGGATAAACAAACGATTGTCCTCAGCTGCAGAATCATTAAATATAAATAAA
  AAAGTTACTACTCACACACTAAGGCATACACACATAAGTTTATTGGCGGAAATGAATATA
  TCGTTAAAAGCAATTATGAAAAGAGTAGGACATACAGATGAAAAAACGACTATAAAGGTA
  TATACCCATGTAACAGAGAAAATGGACAGAGAGTTAGAGCAAAAATTAGAAAAACTTGTG
  TACTAA
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  1206
  

⊟Protein

⊟General

• locus tag: SACOL_RS01605 [old locus tag: SACOL0318 ]
• symbol: SACOL_RS01605
• description: site-specific integrase
• length: 401
• theoretical pI: 10.3392
• theoretical MW: 46973.1
• GRAVY: -0.697756

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 73.7)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 71.5)
  and 2 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 54.9)
  Genetic information processing Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 54.9)
• TheSEED: see SACOL0318
• PFAM:
  DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 99.5)
  and 3 more
  l-integrase_N (CL0469) Phage_int_SAM_3; Phage integrase, N-terminal SAM-like domain (PF14659; HMM-score: 23.5)
  HIT (CL0265) DcpS_C; Scavenger mRNA decapping enzyme C-term binding (PF11969; HMM-score: 15.8)
  l-integrase_N (CL0469) Phage_int_SAM_1; Phage integrase, N-terminal SAM-like domain (PF02899; HMM-score: 14.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.01108
  • TAT(Tat/SPI): 0.000421
  • LIPO(Sec/SPII): 0.003012
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446186332 NCBI
• RefSeq: WP_000264187 NCBI
• UniProt: see SACOL0318

⊟Protein sequence

• MWFEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQKEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGFKVTTLDNLKTRIKNIKKNSSQNLLLNKIDTKYMQTFINELSNVYSANQVKRQLGHMKEAIKYAVKFYNYPNEHILNSVTLPKKSKTIEDIEKEEAKMYNYLEMEQVIQIRDFILNDNNMQYRARILVAGAVEVQALTGMRIGELLALQVKDVDLKNKTIDINGTIHRIKCNAGFGHKDTTKTAGSKRKIAINSRIANVLKKIMLENKKMQQWEPSYVDRGFIFTTCQGNPMQGSRINKRLSSAAESLNINKKVTTHTLRHTHISLLAEMNISLKAIMKRVGHTDEKTTIKVYTHVTEKMDRELEQKLEKLVY

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: see SACOL0318
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for NCTC8325

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications