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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1438 [new locus tag: SAUSA300_RS07855 ]
  • pan locus tag?: SAUPAN001246000
  • symbol: SAUSA300_1438
  • pan gene symbol?: int1
  • synonym:
  • product: phiSLT ORF401-like protein, integrase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1438 [new locus tag: SAUSA300_RS07855 ]
  • symbol: SAUSA300_1438
  • product: phiSLT ORF401-like protein, integrase
  • replicon: chromosome
  • strand: +
  • coordinates: 1590733..1591938
  • length: 1206
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTGGATTGAAAAATTTAAAAACAAAAATAACGAAACTAAATACAGATACTACGAGAAG
    TATAAAGATCCATACACAGATAAATGGAAGCGCGTAAGTGTTGTGTTGAACAAGAATACA
    AAACAATCTCAAAAAGAAGCAATGTTTCGTTTAGAAGAAAAAATAAAAGAAAAACTGAAC
    AACAAGTCGTCAAGCGAATTAAAAACTTTGACTTTTCACGCGCTATTAGATGAATGGCTT
    GAATATCATATAAAAACATCAGGTTCAAAGTTGACTACTCTTAATAATATAAAAATAAGA
    ATTAGAAACATTAAACGATACAGCTCTGAGAACTTGCTTTTAAACAAACTAGATACAAAA
    TATATGCAGATATTTATTAATAAATTATCAGATATCTATTCTCAAAATCAAGTAACCCGT
    CAACTCGGAGATATGAAAGGAGCTATTAAATATGCAGTTAAATTTTACAATTATCCAAAT
    GAATATTTGTTAACTAATGTCAAAATTCCTAAAAGAAGAAAAACAATAGAGGATATCGAA
    AAAGATGAATCTAAAATGTACAACTATTTAGAAATGAACCAAGTCCTACAGATACGTGAT
    CATATACTAAATGATAATAAGTTACACAAGCGAAATCGCATTTTAATTGCCAGCATCTTA
    GAAGTACAGGCTTTAACTGGTATGCGCATAGGAGAACTACAAGCACTGCAGGAAAAAGAT
    ATAGATTTATTAAACAAAACTATCAATATAACAGGTACAATTCACCGCATTAAATACGAG
    GAAGGATTCGGATACAAAGACACTACAAAGACTATAAGTTCAAAAAGAAGTATCAGCATC
    AATTCTAGAACCGTAGAAATTTTTAAAAAGATAATACTGGAAAACAAAATGTTGAAAAGA
    TGGAATTCGAGCTATGTTGACAGAGGGTTCATATTCACAACAAAAAAAGGGAATCCTTTA
    TGTAATAATCAAATCGCCGGTGTGCTTAAGAAAACTACAAAAGCTTTAAATATGAATAAG
    AAAGTTACCACGCACACATTTAGACATACACACATAACTTTATTAGTAGAAATGAATGTT
    TCTTTAAAAGCAATTATGAAAAGGGTAGGACATGTAGATGAAAAAACAACCATTCGCATA
    TATACTCATGTAACTGAAAAAATGGATAGAGAACTAACTCAAAAACTCGAAAACATTCCA
    AGTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1206

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1438 [new locus tag: SAUSA300_RS07855 ]
  • symbol: SAUSA300_1438
  • description: phiSLT ORF401-like protein, integrase
  • length: 401
  • theoretical pI: 10.4693
  • theoretical MW: 47503.9
  • GRAVY: -0.709726

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 76.9)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 68.1)
    and 3 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 48.6)
    Genetic information processing Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 48.6)
    Cellular processes Cellular processes Sporulation and germination sporulation protein YabP (TIGR02892; HMM-score: 13.8)
  • TheSEED  :
    • Phage integrase
  • PFAM:
    DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 94.4)
    and 1 more
    HIT (CL0265) DcpS_C; Scavenger mRNA decapping enzyme C-term binding (PF11969; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011252
    • TAT(Tat/SPI): 0.00039
    • LIPO(Sec/SPII): 0.002927
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQKEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIRNIKRYSSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPNEYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIASILEVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKRSISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTKALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDRELTQKLENIPS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

This is the integrase for prophage ΦFPR2. It is a site-specific class Sa2int integrase that recognizes the 29-bp attB sequence AAATAAACATATCGTCATAATGTGATGGT and catalyzes either integration or excision of a prophage, typically between two copies of the attB sequence. The classical attB sequence for Sa2int prophage is located within a hypothetical lipoprotein found immediately downstream of the srrB staphylococcal respiratory response sensor histidine kinase. However, the integrase can accommodate an impressive number of mismatches and can integrate in several other locations such as within lacG (GTATTCCAATCACATCATAACATGATGGT), sea (CATTTTGTTCATCATCATTATAATATTTA) or elsewhere, albeit at lower efficiency. Some S. aureus strains will have two similar Sa2int prophage at both lacG and srrB integration sites. Sa2int prophage frequently carry coding sequences for the lukFPV lukSPV Panton-Valentine leukocidins.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Christiane Goerke, Roman Pantucek, Silva Holtfreter, Berit Schulte, Manuel Zink, Dorothee Grumann, Barbara M Bröker, Jiri Doskar, Christiane Wolz
    Diversity of prophages in dominant Staphylococcus aureus clonal lineages.
    J Bacteriol: 2009, 191(11);3462-8
    [PubMed:19329640] [WorldCat.org] [DOI] (I p)    Douglas R Deutsch, Bryan Utter, Kathleen J Verratti, Heike Sichtig, Luke J Tallon, Vincent A Fischetti
    Extra-Chromosomal DNA Sequencing Reveals Episomal Prophages Capable of Impacting Virulence Factor Expression in Staphylococcus aureus.
    Front Microbiol: 2018, 9;1406
    [PubMed:30013526] [WorldCat.org] [DOI] (P e)    S Narita, J Kaneko, J Chiba, Y Piémont, S Jarraud, J Etienne, Y Kamio
    Phage conversion of Panton-Valentine leukocidin in Staphylococcus aureus: molecular analysis of a PVL-converting phage, phiSLT.
    Gene: 2001, 268(1-2);195-206
    [PubMed:11368915] [WorldCat.org] [DOI] (P p)    Christiane Wirtz, Wolfgang Witte, Christiane Wolz, Christiane Goerke
    Insertion of host DNA into PVL-encoding phages of the Staphylococcus aureus lineage ST80 by intra-chromosomal recombination.
    Virology: 2010, 406(2);322-7
    [PubMed:20708208] [WorldCat.org] [DOI] (I p)
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