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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0664 [new locus tag: SACOL_RS03435 ]
  • pan locus tag?: SAUPAN002475000
  • symbol: SACOL0664
  • pan gene symbol?:
  • synonym:
  • product: endonuclease III

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0664 [new locus tag: SACOL_RS03435 ]
  • symbol: SACOL0664
  • product: endonuclease III
  • replicon: chromosome
  • strand: +
  • coordinates: 692691..693326
  • length: 636
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTTAGGAACTGATGAATTATATAAAGTTTTATATGAACATCTCGGACCACAATTTTGG
    TGGCCTGCTGATAATGACATTGAAATGATGTTAGGTGCAATTTTAGTTCAAAATACTAGA
    TGGCGAAATGCAGAAATTGCATTGAATCAGATTAAAGAACATACGCATTTTAATCCAAAT
    CATATATTAGAACTACCTATTGAAACGTTACAATCATTGATACATTCAAGTGGCTTTTAT
    AAAAGTAAATCACTGACGATTAAAACATTATTAACATGGTTAGCACGACATCATTTCAAT
    TATCAAGAGATTAATGAGCGATATAAAGGTGGATTAAGAAAAGAATTATTATCTTTGAAA
    GGTATTGGAAGTGAAACAGCAGATGTCTTACTTGTTTATATATTCGGACGTATTGAATTT
    ATTCCAGATAGCTATACAAGAAAAATATATGATAAATTAGGATATGAAAACACTAAAAAT
    TATGATCAATTAAAAAAAGTAGTCACATTACCAAATCATTTTACAAATCAAGATGCTAAT
    GAATTTCATGCTCTGTTAGATGTATTTGGTAAACATTACTTTAGAGACAAAGATATAAAG
    AATTATGATTTTTTAGAACCTTACTTTAAAAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    636

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL0664 [new locus tag: SACOL_RS03435 ]
  • symbol: SACOL0664
  • description: endonuclease III
  • length: 211
  • theoretical pI: 7.4149
  • theoretical MW: 25016.4
  • GRAVY: -0.492417

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair endonuclease III (TIGR01083; EC 4.2.99.18; HMM-score: 46.9)
    and 1 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair 8-oxoguanine DNA-glycosylase (ogg) (TIGR00588; EC 3.2.2.-; HMM-score: 16)
  • TheSEED  :
    • Endonuclease III (EC 4.2.99.18)
    DNA Metabolism DNA repair DNA Repair Base Excision  Endonuclease III (EC 4.2.99.18)
  • PFAM:
    HHH (CL0198) HhH-GPD; HhH-GPD superfamily base excision DNA repair protein (PF00730; HMM-score: 32.2)
    and 1 more
    HHH; Helix-hairpin-helix motif (PF00633; HMM-score: 21.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001499
    • TAT(Tat/SPI): 0.000122
    • LIPO(Sec/SPII): 0.000247
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MLGTDELYKVLYEHLGPQFWWPADNDIEMMLGAILVQNTRWRNAEIALNQIKEHTHFNPNHILELPIETLQSLIHSSGFYKSKSLTIKTLLTWLARHHFNYQEINERYKGGLRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYDKLGYENTKNYDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]