From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0696 [new locus tag: SACOL_RS03580 ]
  • pan locus tag?: SAUPAN002515000
  • symbol: tagB
  • pan gene symbol?: tagB
  • synonym:
  • product: teichoic acid biosynthesis protein B

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0696 [new locus tag: SACOL_RS03580 ]
  • symbol: tagB
  • product: teichoic acid biosynthesis protein B
  • replicon: chromosome
  • strand: +
  • coordinates: 720191..721294
  • length: 1104
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAACGTTTTAATAAAGAAATTTTATCATTTGGTAGTTCGAATACTTTCTAAAATGATT
    ACGCCTCAAGTGATTGATAAACCGCATATCGTATTTATGATGACTTTTCCAGAAGATATT
    AAGCCTATCATCAAAGCATTAAATAATTCGTCGTATCAGAAAACTGTTTTAACAACACCA
    AAACAAGCGCCTTATTTATCTGAACTTAGCGACGATGTTGATGTGATAGAAATGACTAAT
    CGAACATTGGTAAAACAAATTAAGGCTTTGAAAAGCGCGCAGATGATTATTATCGATAAT
    TATTACCTATTACTAGGTGGATATAATAAGACTTCTAATCAACACATTGTTCAAACGTGG
    CATGCAAGTGGTGCATTAAAAAACTTTGGCTTAACAGATCATCAAGTCGATGTGTCTGAC
    AAGGCAATGGTTCAGCAGTACCGTAAAGTTTATCAAGCGACGGATTTTTACTTAGTGGGT
    TGTGAACAAATGTCACAATGTTTTAAACAATCTTTAGGTGCAACAGAAGAGCAAATGCTG
    TATTTTGGGCTTCCGAGAATTAATAAATATTACACAGCTGATAGAGCAACGGTTAAGGCA
    GAGTTAAAGGATAAATATGGAATTACAAATAAGTTGGTATTATATGTACCAACATATAGA
    GAAGATAAAGCAGATAATAGGGCTATTGATAAAGCTTATTTTGAAAAATGTTTACCAGGA
    TATACACTGATTAATAAATTACATCCATCAATTGAAGATTCAGACATTGATGACGTATCT
    TCAATCGACACGTCTACATTAATGCTAATGTCAGATATAATTATTAGCGACTATAGTTCG
    CTGCCAATAGAAGCTAGCTTGTTAGATATTCCAACTATATTTTATGTGTATGATGAAGGA
    ACATATGATCAGGTGAGAGGCCTGAATCAATTTTACAAAGCAATACCGGATAGCTACAAA
    GTGTATACTGAAGAAGATTTAATAATGACGATACAAGAAAAAGAACATCTATTAAGTCCG
    TTATTTAAAGATTGGCATAAGTATAATACTGATAAAAGTTTACATCAGCTCACAGAATAT
    ATAGATAAGATGGTGACAAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1104

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL0696 [new locus tag: SACOL_RS03580 ]
  • symbol: TagB
  • description: teichoic acid biosynthesis protein B
  • length: 367
  • theoretical pI: 6.24812
  • theoretical MW: 42435.6
  • GRAVY: -0.291008

Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides surface carbohydrate biosynthesis protein (TIGR04396; HMM-score: 21.6)
  • TheSEED:  
    Cell Wall and Capsule Gram-Positive cell wall components Teichoic and lipoteichoic acids biosynthesis  CDP-glycerol: N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenyl glycerophosphotransferase
  • PFAM:
    GT-B (CL0113) Glyphos_transf; CDP-Glycerol:Poly(glycerophosphate) glycerophosphotransferase (PF04464; HMM-score: 149.2)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.042698
    • TAT(Tat/SPI): 0.000288
    • LIPO(Sec/SPII): 0.000804
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPIIKALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYYLLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVGCEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLVLYVPTYREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEDSDIDDVSSIDTSTLMLMSDIIISDYSSLPIEASLLDIPTIFYVYDEGTYDQVRGLNQFYKAIPDSYKVYTEEDLIMTIQEKEHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK

Experimental data[edit | edit source]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]