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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0771 [new locus tag: SACOL_RS03960 ]
  • pan locus tag?: SAUPAN002597000
  • symbol: SACOL0771
  • pan gene symbol?: queD
  • synonym:
  • product: 6-pyruvoyl tetrahydrobiopterin synthase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0771 [new locus tag: SACOL_RS03960 ]
  • symbol: SACOL0771
  • product: 6-pyruvoyl tetrahydrobiopterin synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 792382..792801
  • length: 420
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGTTACAACAAATCTATCCTAGTACAACGCATCCATATCAATTCGAATTAAATAAAGAT
    TTTAATTTTTCGGCTGCACATCACATTCCATGTGAAGAAGCAGGTATTTGTCAAAATGTC
    CATGGTCATACTTACTTTGTTAATTTAACAATTGTCGGTGATAAACTAGATGACACTGGC
    TTCTTAGTGAACTTTAGCCATTTGAAAAAGATGATACACGGTAAATTTGACCATCAACTG
    TTAAATAACTTACCTGCTTTTAAAAACAAAATCCCTTCAACTGAAATCGTAGCGGAAACA
    ATTTATCAAATTGTTAAAGAAAATTTGGCATCGCTCGAACACCAACCAAAATGTATTCAA
    GTATTTGTAAGAGAAACACCAACAAGTTATGTCGTATTTAGACCAAAGGAACAGGTGTAA
    60
    120
    180
    240
    300
    360
    420

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL0771 [new locus tag: SACOL_RS03960 ]
  • symbol: SACOL0771
  • description: 6-pyruvoyl tetrahydrobiopterin synthase
  • length: 139
  • theoretical pI: 7.01376
  • theoretical MW: 16011.2
  • GRAVY: -0.274101

Function[edit | edit source]

  • reaction:
    EC 4.1.2.50  ExPASy
    6-carboxytetrahydropterin synthase 7,8-dihydroneopterin 3'-triphosphate + H2O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + triphosphate?
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification 6-pyruvoyl tetrahydropterin synthase/QueD family protein (TIGR00039; HMM-score: 100.7)
    Genetic information processing Protein synthesis tRNA and rRNA base modification queuosine biosynthesis protein QueD (TIGR03367; HMM-score: 93.8)
    and 1 more
    6-pyruvoyl tetrahydropterin synthase-related domain (TIGR03112; HMM-score: 30.8)
  • TheSEED:  
    RNA Metabolism RNA processing and modification Queuosine-Archaeosine Biosynthesis  Queuosine biosynthesis QueD, PTPS-I
    and 2 more
    Queuosine exploration RZ  Queuosine biosynthesis QueD, PTPS-I
    Test Q  Queuosine biosynthesis QueD, PTPS-I
  • PFAM:
    THBO-biosyn (CL0334) PTPS; 6-pyruvoyl tetrahydropterin synthase (PF01242; HMM-score: 105.2)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007616
    • TAT(Tat/SPI): 0.000436
    • LIPO(Sec/SPII): 0.00065
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHTYFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIYQIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV

Experimental data[edit | edit source]

  • experimental localization: Cytoplasmic [1]
    quantitative data / protein copy number per cell: 73 [2]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulators: PreQ1 riboswitch (transcription termination) regulon, SigB* (activation) regulon
    PreQ1 riboswitch(RNA)important in Queuosine biosynthesis; transcription unit transferred from N315 data RegPrecise  
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation [3]   other strains

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: 15.91 h [4]

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  2. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  3. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  4. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]