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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0772 [new locus tag: SACOL_RS03965 ]
  • pan locus tag?: SAUPAN002598000
  • symbol: SACOL0772
  • pan gene symbol?: queC
  • synonym:
  • product: exsB protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0772 [new locus tag: SACOL_RS03965 ]
  • symbol: SACOL0772
  • product: exsB protein
  • replicon: chromosome
  • strand: -
  • coordinates: 792803..793471
  • length: 669
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGAAAGTGTATTAAATAATGAAAAAGCCATCGTTGTCTTTAGTGGTGGTCAAGACAGT
    ACAACATGTCTCTTTTATGCAAAAAAACATTTCAAAGAAGTTGAACTCGTAACGTTTAAT
    TATGGCCAAAGACATGATACTGAAATTGAAGTCGCAAAACAAATTGCACAAGATCAAGGA
    ATGAAACATCACGTTTTAGATATGTCATTATTATCACAACTTACTCCAAACGCATTAACA
    CAACATGATATGGAAATTACTAATAATGAAGATGGTATACCTAATACATTTGTTCCAGCT
    AGAAATTTACTTTTCTTGTCGTTTGCAGGCGCTCTAGCTTATCAAATTGGGGCTAAGCAT
    ATTATTACAGGCGTATGTGAAACAGACTTTTCAGGCTACCCAGACTGTCGCGATAGTTTT
    ATAAAATCAATGAACGTAACATTAAGCCTAGCTATGGACAAAGATTTTGTCATTCATACT
    CCTTTAATGTGGTTAAACAAAGCAGAAACGTGGAAATTAAGTGATGAACTCGAAGTTTTA
    GATTATATTCGTACAAAAACATTAACATGCTATAACGGTATCATTGGGGATGGCTGTGGT
    GAATGTCCAGCTTGTCATTTACGTCAACGTGGACTAAATCAATATCTTGAAAGTAAAGGA
    GCGCTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    669

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0772 [new locus tag: SACOL_RS03965 ]
  • symbol: SACOL0772
  • description: exsB protein
  • length: 222
  • theoretical pI: 5.27733
  • theoretical MW: 24887.2
  • GRAVY: -0.186937

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.4.20?  ExPASy
    7-cyano-7-deazaguanine synthase 7-carboxy-7-carbaguanine + NH3 + ATP = 7-cyano-7-carbaguanine + ADP + phosphate + H2O
  • TIGRFAM:
    Unknown function General queuosine biosynthesis protein QueC (TIGR00364; HMM-score: 278.9)
    and 11 more
    Hypothetical proteins Conserved TIGR00268 family protein (TIGR00268; HMM-score: 26.1)
    Metabolism Amino acid biosynthesis Aspartate family asparagine synthase (glutamine-hydrolyzing) (TIGR01536; EC 6.3.5.4; HMM-score: 26)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 23.4)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 21.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 21.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides NAD+ synthetase (TIGR00552; EC 6.3.1.5; HMM-score: 20.7)
    Metabolism Amino acid biosynthesis Glutamate family argininosuccinate synthase (TIGR00032; EC 6.3.4.5; HMM-score: 17.2)
    MJ0570-related uncharacterized domain (TIGR00290; HMM-score: 14.7)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), C-terminal domain (TIGR00884; EC 6.3.5.2; HMM-score: 13.4)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (TIGR00420; EC 2.1.1.61; HMM-score: 12.6)
    Metabolism Central intermediary metabolism Sulfur metabolism sulfate adenylyltransferase, small subunit (TIGR02039; EC 2.7.7.4; HMM-score: 12.5)
  • TheSEED  :
    • 7-cyano-7-deazaguanine synthase (EC 6.3.4.20)
    Miscellaneous Miscellaneous - no subcategory EC 6.3.4.- Ligases that form carbon-nitrogen bonds  Queuosine Biosynthesis QueC ATPase
    and 1 more
    RNA Metabolism RNA processing and modification Queuosine-Archaeosine Biosynthesis  Queuosine Biosynthesis QueC ATPase
  • PFAM:
    HUP (CL0039) QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 291.9)
    and 8 more
    Asn_synthase; Asparagine synthase (PF00733; HMM-score: 24.8)
    ATP_bind_3; PP-loop family (PF01171; HMM-score: 21.7)
    NAD_synthase; NAD synthase (PF02540; HMM-score: 19)
    PAPS_reduct; Phosphoadenosine phosphosulfate reductase family (PF01507; HMM-score: 17.8)
    Arginosuc_synth; Arginosuccinate synthase (PF00764; HMM-score: 16.8)
    ThiI; Thiamine biosynthesis protein (ThiI) (PF02568; HMM-score: 15.3)
    Diphthami_syn_2; Diphthamide synthase (PF01902; HMM-score: 14.2)
    TIM_barrel (CL0036) AP_endonuc_2; Xylose isomerase-like TIM barrel (PF01261; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005103
    • TAT(Tat/SPI): 0.000115
    • LIPO(Sec/SPII): 0.000828
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQRHDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARNLLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHTPLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLESKGAL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: PreQ1 riboswitch (transcription termination) regulon
    PreQ1 riboswitch(RNA)important in Queuosine biosynthesis; regulatory site identified based on RegPrecise data for N315 RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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