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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL0778 [new locus tag: SACOL_RS04000 ]
- pan locus tag?: SAUPAN002605000
- symbol: SACOL0778
- pan gene symbol?: ltaS
- synonym:
- product: sulfatase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL0778 [new locus tag: SACOL_RS04000 ]
- symbol: SACOL0778
- product: sulfatase
- replicon: chromosome
- strand: +
- coordinates: 798690..800630
- length: 1941
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3237805 NCBI
- RefSeq: YP_185653 NCBI
- BioCyc: see SACOL_RS04000
- MicrobesOnline: 912251 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1921ATGAGTTCACAAAAAAAGAAAATTAGTCTTTTTGCGTTCTTCTTATTAACCGTAATAACG
ATTACCTTGAAGACGTATTTTTCTTATTATGTTGATTTTTCTTTAGGTGTTAAAGGTTTA
GTACAAAACTTAATATTATTGATGAATCCTTATAGTTTAGTAGCACTGGTTTTAAGTGTG
TTCCTATTCTTTAAAGGCAAAAAAGCATTTTGGTTCATGTTCATAGGCGGCTTCTTATTG
ACGTTCCTATTATATGCCAATGTTGTGTACTTTAGATTCTTCTCTGATTTTTTAACGTTT
AGTACTTTAAACCAAGTAGGTAACGTAGAATCTATGGGTGGTGCGGTTAGTGCATCATTC
AAATGGTATGACTTTGTTTATTTCATTGATACGTTAGTTTACTTATTCATTTTAATATTT
AAAACAAAATGGTTAGACACAAAAGCATTTAGTAAGAAATTTGTTCCTGTCGTAATGGCG
GCTTCAGTAGCATTATTCTTCTTAAACTTAGCTTTTGCTGAAACTGACAGACCAGAATTA
TTAACACGTACATTTGACCATAAATATTTAGTGAAATATTTAGGACCTTATAACTTTACA
GTATACGATGGTGTTAAAACTATCGAAAATAATCAACAAAAAGCGCTAGCATCTGAAGAT
GACTTAACAAAAGTATTAAATTATACGAAACAACGTCAAACAGAGCCTAACCCAGAATAT
TATGGGGTGGCAAAGAAGAAAAATATTATTAAGATTCATTTAGAAAGTTTCCAAACCTTC
TTAATTAATAAAAAGGTTAATGGTAAAGAAGTAACACCGTTTTTAAACAAATTATCAAGT
GGGAAAGAGCAATTCACATACTTCCCTAACTTTTTCCATCAAACAGGTCAAGGTAAAACA
TCTGACTCTGAATTTACAATGGATAACAGTTTATACGGTTTACCGCAAGGTTCTGCCTTT
TCATTAAAAGGAGATAATACGTATCAGTCATTACCAGCAATTTTAGATCAAAAGCAAGGC
TACAAATCTGATGTCATGCACGGTGACTATAAAACATTCTGGAACAGAGACCAAGTATAT
AAACACTTTGGTATCGATAAATTCTATGATGCAACATACTATGACATGTCAGATAAAAAC
GTTGTAAACTTAGGCTTGAAAGACAAAATTTTCTTTAAAGATTCTGCTAATTATCAAGCT
AAGATGAAATCACCATTCTATTCTCATTTAATTACATTGACTAACCACTATCCATTCACA
TTAGATGAAAAGGATGCAACTATTGAGAAATCAAACACAGGTGATGCAACAGTTGATGGT
TATATTCAAACAGCACGTTATTTAGACGAAGCATTAGAAGAATATATTAATGACTTGAAG
AAAAAAGGATTATATGACAATTCAGTGATTATGATTTATGGTGACCACTATGGTATCTCT
GAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTT
ACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAAT
AATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTACATTTGGCTGGTATAGAT
ACGAAGAACTATTTAATGTTCGGTACTGATTTATTCTCTAAAGGTCATAATCAAGTAGTT
CCATTCAGAAATGGTGACTTTATAACAAAAGATTATAAATATGTTAATGGTAAGATTTAT
TCTAATAAAAATAATGAACTCATAACTACTCAACCAGCTGATTTCGAAAAGAATAAAAAG
CAAGTTGAAAAGGATCTCGAAATGAGTGACAACGTGCTTAATGGTGATTTGTTTAGATTC
TACAAAAATCCAGACTTCAAAAAGGTAAATCCTTCGAAGTATAAATATGAAACAGGACCT
AAAGCAAACTCTAAAAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL0778 [new locus tag: SACOL_RS04000 ]
- symbol: SACOL0778
- description: sulfatase
- length: 646
- theoretical pI: 9.4708
- theoretical MW: 74399.6
- GRAVY: -0.317647
⊟Function[edit | edit source]
- reaction: EC 2.7.8.-? ExPASy
- TIGRFAM: choline-sulfatase (TIGR03417; EC 3.1.6.6; HMM-score: 20.4)and 1 morefrataxin (TIGR03422; HMM-score: 12.3)
- TheSEED :
- Lipoteichoic acid synthase LtaS Type Ib
- PFAM: Alk_phosphatase (CL0088) Sulfatase; Sulfatase (PF00884; HMM-score: 113)and 3 moreDUF229; Protein of unknown function (DUF229) (PF02995; HMM-score: 19.8)Phosphodiest; Type I phosphodiesterase / nucleotide pyrophosphatase (PF01663; HMM-score: 18.8)DUF1501; Protein of unknown function (DUF1501) (PF07394; HMM-score: 13.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 4
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0
- Signal peptide possibility: 0.5
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.008585
- TAT(Tat/SPI): 0.000154
- LIPO(Sec/SPII): 0.048829
- predicted transmembrane helices (TMHMM): 5
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNLILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFSTLNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMAASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALASEDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLNKLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAILDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFFKDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDEALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGFWIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGDFITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNPDFKKVNPSKYKYETGPKANSKK
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Integral membrane [1] [2] [3] [4]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
J Proteome Res: 2010, 9(3);1579-90
[PubMed:20108986] [WorldCat.org] [DOI] (I p) - ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)