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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0799 [new locus tag: SACOL_RS04110 ]
  • pan locus tag?: SAUPAN002640000
  • symbol: SACOL0799
  • pan gene symbol?: sstD
  • synonym:
  • product: transferrin receptor

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0799 [new locus tag: SACOL_RS04110 ]
  • symbol: SACOL0799
  • product: transferrin receptor
  • replicon: chromosome
  • strand: +
  • coordinates: 822428..823456
  • length: 1029
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAGAAAACAGTCTTATATTTAGTATTAGCAGTAATGTTTTTATTAGCGGCATGCGGT
    AACAATTCTGATAAAGAACAATCAAAATCAGAAACTAAAGGTTCTAAAGATACAGTAAAA
    ATTGAAAATAACTATAAAATGCGTGGCGAGAAAAAAGATGGTAGTGACGCTAAAAAAGTT
    AAAGAAACTGTTGAAGTACCAAAAAATCCTAAAAATGCAGTTGTGTTAGACTATGGCGCA
    TTAGATGTAATGAAAGAAATGGGCTTATCAGATAAAGTAAAAGCATTACCTAAAGGGGAA
    GGCGGTAAGTCATTACCGAATTTCTTAGAATCATTTAAAGATGATAAATATACAAACGTT
    GGTAATTTAAAAGAAGTGAATTTTGATAAAATTGCTGCGACGAAACCCGAAGTAATCTTT
    ATCTCTGGACGTACAGCTAATCAAAAGAATTTAGATGAATTCAAAAAAGCTGCACCTAAA
    GCGAAAATTGTTTATGTTGGTGCAGATGAAAAGAACTTAATTGGTTCAATGAAACAAAAC
    ACTGAAAATATCGGAAAAATTTACGATAAAGAAGATAAAGCTAAAGAATTAAATAAAGAT
    TTAGATAACAAAATTGCTTCAATGAAAGATAAAACGAAAAACTTCAATAAAACTGTTATG
    TATTTACTAGTTAACGAAGGTGAATTATCAACATTTGGACCTAAAGGTCGTTTTGGTGGA
    TTAGTTTACGATACATTAGGATTCAATGCAGTTGATAAAAAAGTAAGTAATAGCAATCAT
    GGACAAAATGTTTCTAACGAATATGTTAATAAAGAAAATCCAGATGTTATTTTAGCGATG
    GATAGAGGTCAAGCGATAAGTGGTAAATCAACTGCGAAACAAGCATTAAATAATCCTGTA
    TTAAAAAATGTTAAAGCAATTAAAGAAGACAAAGTATATAATTTAGATCCTAAATTATGG
    TACTTTGCAGCTGGATCAACTACAACTACAATTAAACAAATTGAGGAACTTGATAAAGTT
    GTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1029

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0799 [new locus tag: SACOL_RS04110 ]
  • symbol: SACOL0799
  • description: transferrin receptor
  • length: 342
  • theoretical pI: 9.92882
  • theoretical MW: 37854.1
  • GRAVY: -0.652632

⊟Function[edit | edit source]

  • TIGRFAM:
    heme ABC transporter, heme-binding protein isdE (TIGR03659; HMM-score: 76.6)
    and 3 more
    putative ABC transporter PGF-CTERM-modified substrate-binding protein (TIGR04281; HMM-score: 50.6)
    proposed F420-0 ABC transporter, periplasmic F420-0 binding protein (TIGR03868; HMM-score: 23.9)
    Cellular processes Cellular processes Sporulation and germination sporulation lipoprotein, YhcN/YlaJ family (TIGR02898; HMM-score: 15.6)
  • TheSEED  :
    • Iron compound ABC uptake transporter substrate-binding protein
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Iron compound ABC uptake transporter substrate-binding protein
    and 1 more
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Iron acquisition in Streptococcus  Iron compound ABC uptake transporter substrate-binding protein
  • PFAM:
    Chelatase (CL0043) Peripla_BP_2; Periplasmic binding protein (PF01497; HMM-score: 107.4)
    and 1 more
    no clan defined Formyl_trans_N; Formyl transferase (PF00551; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helix: 1
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: FLLAACG
  • SignalP: Signal peptide LIPO(Sec/SPII) length 18 aa
    • SP(Sec/SPI): 0.000351
    • TAT(Tat/SPI): 0.000056
    • LIPO(Sec/SPII): 0.999451
    • Cleavage Site: CS pos: 18-19. LAA-CG. Pr: 0.9999
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENNYKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGGKSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPKAKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKTVMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDVILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQIEELDKVVK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Lipoprotein [1] [2] [3] [4]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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