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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL0800 [new locus tag: SACOL_RS04115 ]
- pan locus tag?: SAUPAN002641000
- symbol: SACOL0800
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL0800 [new locus tag: SACOL_RS04115 ]
- symbol: SACOL0800
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 823773..824087
- length: 315
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3236992 NCBI
- RefSeq: YP_185674 NCBI
- BioCyc: see SACOL_RS04115
- MicrobesOnline: 912272 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
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241
301ATGCCTAAAGTTTATGGTTCATTAATCGATACTGAAACACGCTGTCGCCATTATTTTACC
GAAGAAGATATTATTGCTATTAAATTTAAATGTTGTAATAAATACTATCCATGCTATAAG
TGCCATAATGAGTTTGAAAAGCACGCCATTAAGCGTTGGTCTGAGCCTTCATTTAACGAA
AAAGCAATCTTATGCGGTGTATGCAAACACGAATTAACAATCAATGAATATATGATGGTA
GAGCGTTGTCCAAATTGCCAATCTCGGTTTAACAATCGCTGTAAATATCACTATCATATT
TATTTTGAAATTTAA60
120
180
240
300
315
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL0800 [new locus tag: SACOL_RS04115 ]
- symbol: SACOL0800
- description: hypothetical protein
- length: 104
- theoretical pI: 8.09137
- theoretical MW: 12723.7
- GRAVY: -0.645192
⊟Function[edit | edit source]
- TIGRFAM: MJ0042 family finger-like domain (TIGR02098; HMM-score: 11)and 1 moreProtein fate Protein modification and repair hydrogenase nickel insertion protein HypA (TIGR00100; HMM-score: 7.4)
- TheSEED :
- FIG01108317: hypothetical protein
- PFAM: no clan defined zf-CHY; CHY zinc finger (PF05495; HMM-score: 48.8)and 4 moreZn_Beta_Ribbon (CL0167) DZR; Double zinc ribbon (PF12773; HMM-score: 16.1)no clan defined HypA; Hydrogenase/urease nickel incorporation, metallochaperone, hypA (PF01155; HMM-score: 13.3)Evr1_Alr; Erv1 / Alr family (PF04777; HMM-score: 7.1)Zn_Beta_Ribbon (CL0167) DUF1451; Zinc-ribbon containing domain (PF07295; HMM-score: 6.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.183656
- TAT(Tat/SPI): 0.000783
- LIPO(Sec/SPII): 0.009195
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNEFEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYFEI
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Cytoplasmic [1] [2] [3]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
J Proteome Res: 2010, 9(3);1579-90
[PubMed:20108986] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)