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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0805 [new locus tag: SACOL_RS04140 ]
  • pan locus tag?: SAUPAN002648000
  • symbol: SACOL0805
  • pan gene symbol?: —
  • synonym:
  • product: glycerate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0805 [new locus tag: SACOL_RS04140 ]
  • symbol: SACOL0805
  • product: glycerate kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 827570..828694
  • length: 1125
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAAAGTATTAGTAGCCATGGATGAGTTTCATGGAATTATTTCAAGTTATCAAGCTAAT
    AGATATGTTGAAGAGGCAGTTGCAAGCCAAATTGAAACTGCAGATGTAGTTCAAGTACCA
    TTGTTTAATGGAAGACATGAATTATTAGATTCTGTATTTTTATGGCAATCTGGGCAAAAG
    TATCGTATACCAGTACATGATGCAGATATGAATGAAGTTGAAGGTGTTTACGGACAAACT
    GATACAGGGATGACCGTTATCGAGGGGAATTTATTTTTAAAAGGTAAAAAACCAATTGTT
    GAACGAACAAGTTATGGTTTAGGAGAAATGATTAAACATGCATTAGATAACGACGCAAAA
    CATGTTGTAATTTCACTAGGTGGGATTGATAGTTTTGATGCTGGTGCAGGTATGTTACAA
    GCATTAGGTGCTCAATTCTATGATGACGAAGGGCGTGTCGTAGATATGAGACAAGGTGCT
    GGTGTAATTAAATATATTCGTCGTATGGATATGTCGAACTTACACCCTAAAATGGAAACA
    GCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATC
    ATGCAAACTTATGATGCGCATCAGTTGAATCATAATCAAGCAGCAGAAATCGATAATTTA
    ATTTGGTATTTTAGTGAGTTATTTAAAAGTGAATTGAAAATTGCAATTGGTCCAGTTGAA
    CGTGGTGGTGCTGGTGGTGGAATTGCAGCAGTCTTGAATGGACTGTATCAAGCTGAAATA
    TTAACCAGTCATGCATTAGTAGACCAACTAACACATTTAGAAAATTTAGTTGAACAAGCG
    GATTTAATTATTTTTGGAGAAGGATTAAATGAAAATGATCAGTTGCTAGAAACGACAACA
    TTGCGTATTGCAGAACTTTGTCATAAACATCAAAAGGTTGCCATTGCAATTTGTGCAACT
    GCTGAAAAGTTTGATTTATTTGAATCACAAGGGGTTACAGCAATGTTTAATACATTTATC
    GATATGCCAGAAACTTATACTGACTTTAAAATGGGGTTACAAATTAGGCATTATACGGTT
    CAGTCTTTAAAACTGTTGAAAACACATTTTAATGTTGAGGTTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1125

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0805 [new locus tag: SACOL_RS04140 ]
  • symbol: SACOL0805
  • description: glycerate kinase
  • length: 374
  • theoretical pI: 4.87148
  • theoretical MW: 41797.3
  • GRAVY: -0.12246

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Other glycerate kinase (TIGR00045; EC 2.7.1.31; HMM-score: 176.1)
  • TheSEED  :
    • Glycerate kinase (EC 2.7.1.31)
    Amino Acids and Derivatives Alanine, serine, and glycine Glycine and Serine Utilization  Glycerate kinase (EC 2.7.1.31)
    and 5 more
    Carbohydrates Monosaccharides D-galactarate, D-glucarate and D-glycerate catabolism  Glycerate kinase (EC 2.7.1.31)
    Carbohydrates Monosaccharides D-galactarate, D-glucarate and D-glycerate catabolism - gjo  Glycerate kinase (EC 2.7.1.31)
    Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  Glycerate kinase (EC 2.7.1.31)
    Carbohydrates Organic acids Glycerate metabolism  Glycerate kinase (EC 2.7.1.31)
    Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Glycerate kinase (EC 2.7.1.31)
  • PFAM:
    no clan defined Gly_kinase; Glycerate kinase family (PF02595; HMM-score: 187.5)
    and 1 more
    STAS (CL0502) STAS; STAS domain (PF01740; HMM-score: 14.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00405
    • TAT(Tat/SPI): 0.000252
    • LIPO(Sec/SPII): 0.000797
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNGRHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVERTSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGAGVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAHQLNHNQAAEIDNLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENLVEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTAMFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 39.29 h [4]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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