PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0726 [new locus tag: SAUSA300_RS03910 ]
• pan locus tag^?: SAUPAN002648000
• symbol: SAUSA300_0726
• pan gene symbol^?: —
• synonym:
• product: glycerate kinase family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0726 [new locus tag: SAUSA300_RS03910 ]
• symbol: SAUSA300_0726
• product: glycerate kinase family protein
• replicon: chromosome
• strand: +
• coordinates: 805969..807093
• length: 1125
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913665 NCBI
• RefSeq: YP_493429 NCBI
• BioCyc: see SAUSA300_RS03910
• MicrobesOnline: 1292241 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAGTATTAGTAGCCATGGATGAGTTTCATGGAATTATTTCAAGTTATCAAGCTAAT
  AGATATGTTGAAGAGGCAGTTGCAAGCCAAATTGAAACTGCAGATGTAGTTCAAGTACCA
  TTGTTTAATGGAAGACATGAATTATTAGATTCTGTATTTTTATGGCAATCTGGGCAAAAG
  TATCGTATACCAGTACATGATGCAGATATGAATGAAGTTGAAGGTGTTTACGGACAAACT
  GATACAGGGATGACCGTTATCGAGGGGAATTTATTTTTAAAAGGTAAAAAACCAATTGTT
  GAACGAACAAGTTATGGTTTAGGAGAAATGATTAAACATGCATTAGATAACGACGCAAAA
  CATGTTGTAATTTCACTAGGTGGGATTGATAGTTTTGATGCTGGTGCAGGTATGTTACAA
  GCATTAGGTGCTCAATTCTATGATGACGAAGGGCGTGTCGTAGATATGAGACAAGGTGCT
  GGTGTAATTAAATATATTCGTCGTATGGATATGTCGAACTTACACCCTAAAATGGAAACA
  GCAAGAATTCAAGTAATGTCGGATTTTTCAAGTCGATTATATGGTAAGCAAAGTGAAATC
  ATGCAAACTTATGATGCGTATCAGTTGAATCATAATCAAGCAGCAGAAATCGATAATTTA
  ATTTGGTATTTTAGTGAGTTATTTAAAAGTGAATTGAAAATTGCAATTGGTCCAGTTGAA
  CGTGGTGGTGCTGGTGGTGGAATTGCAGCAGTCTTGAATGGACTGTATCAAGCTGAAATA
  TTAACCAGTCATGCATTAGTAGACCAACTAACACATTTAGAAAATTTAGTTGAACAAGCG
  GATTTAATTATTTTTGGAGAAGGATTAAATGAAAATGATCAGTTGCTAGAAACGACAACA
  TTGCGTATTGCAGAACTTTGTCATAAACATCAAAAGGTTGCCATTGCAATTTGTGCAACT
  GCTGAAAAGTTTGATTTATTTGAATCACAAGGGGTTACAGCAATGTTTAATACATTTATC
  GATATGCCAGAAACTTATACTGACTTTAAAATGGGGTTACAAATTAGGCATTATACGGTT
  CAGTCTTTAAAACTGTTGAAAACACATTTTAATGTTGAGGTTTAG
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  1125
  

⊟Protein

⊟General

• locus tag: SAUSA300_0726 [new locus tag: SAUSA300_RS03910 ]
• symbol: SAUSA300_0726
• description: glycerate kinase family protein
• length: 374
• theoretical pI: 4.80225
• theoretical MW: 41823.4
• GRAVY: -0.11738

⊟Function

• reaction:
  EC 2.7.1.31?  ExPASy
  Glycerate 3-kinase ATP + D-glycerate = ADP + 3-phospho-D-glycerate
• TIGRFAM:
  Metabolism Energy metabolism Other glycerate kinase (TIGR00045; EC 2.7.1.31; HMM-score: 175.3)
• TheSEED  :
  • Glycerate kinase (EC 2.7.1.31)
  Amino Acids and Derivatives Alanine, serine, and glycine Glycine and Serine Utilization  Glycerate kinase (EC 2.7.1.31)
  and 5 more
  Carbohydrates Monosaccharides D-galactarate, D-glucarate and D-glycerate catabolism  Glycerate kinase (EC 2.7.1.31)
  Carbohydrates Monosaccharides D-galactarate, D-glucarate and D-glycerate catabolism - gjo  Glycerate kinase (EC 2.7.1.31)
  Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  Glycerate kinase (EC 2.7.1.31)
  Carbohydrates Organic acids Glycerate metabolism  Glycerate kinase (EC 2.7.1.31)
  Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Glycerate kinase (EC 2.7.1.31)
• PFAM:
  no clan defined Gly_kinase; Glycerate kinase family (PF02595; HMM-score: 186.7)
  and 1 more
  STAS (CL0502) STAS; STAS domain (PF01740; HMM-score: 14.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00405
  • TAT(Tat/SPI): 0.000252
  • LIPO(Sec/SPII): 0.000797
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160263 NCBI
• RefSeq: YP_493429 NCBI
• UniProt: A0A0H2XG63 UniProt

⊟Protein sequence

• MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNGRHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVERTSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGAGVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAYQLNHNQAAEIDNLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENLVEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTAMFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications