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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1066 [new locus tag: SACOL_RS05435 ]
  • pan locus tag?: SAUPAN003266000
  • symbol: SACOL1066
  • pan gene symbol?: fmtA
  • synonym:
  • product: fmt protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1066 [new locus tag: SACOL_RS05435 ]
  • symbol: SACOL1066
  • product: fmt protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1074466..1075659
  • length: 1194
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAATTTAATAAAGTAAAACTAGTTATACATGCGTGTGTACTATTATTTATCATTATT
    TCTATAGCGTTAATTTTTCATCGATTACAGACGAAGACACATTCTATAGACCCAATACAT
    AAGGAAACAAAATTATCAGACAATGAAAAATATTTAGTGGATCGTAATAAGGAAAAGGTT
    GCGCCGTCTAAACTAAAAGAGGTATATAATAGCAAGGATCCTAAATATAAGAAAATTGAC
    AAGTATTTACAAAGTTCATTATTTAACGGTTCAGTAGCTATATATGAAAATGGCAAATTG
    AAAATGAGTAAAGGTTATGGATATCAAGATTTTGAAAAAGGTATTAAAAACACACCGAAT
    ACGATGTTTTTAATAGGTTCAGCTCAAAAATTTTCAACAGGGTTACTGTTAAAACAGTTA
    GAAGAAGAACATAAAATAAATATCAATGATCCAGTAAGTAAATACCTTCCATGGTTTAAA
    ACATCTAAGCCTATCCCATTGAAAGATTTAATGTTGCATCAAAGTGGATTATATAAATAT
    AAATCCTCAAAAGATTATAAAAATTTAGATCAAGCAGTTAAAGCGATTCAAAAACGTGGT
    ATTGATCCTAAGAAATACAAAAAGCATATGTATAACGATGGGAATTATTTAGTACTTGCG
    AAAGTAATTGAAGAAGTTACAGGTAAATCTTATGCTGAGAATTATTATACAAAAATAGGA
    GATCCTTTAAAACTTCAGCACACAGCATTTTATGATGAACAACCTTTTAAAAAATATCTA
    GCAAAAGGTTATGCTTATAATAGTACAGGACTTTCATTCTTAAGACCTAATATTTTGGAC
    CAATACTATGGTGCAGGTAATTTATATATGACACCAACAGATATGGGTAAATTAATTACT
    CAAATACAACAATATAAATTATTCAGTCCTAAAATAACCAATCCATTATTACATGAGTTT
    GGTACGAAAAAGTATCCAGATGAATATCGATATGGTTTCTATGCTAAGCCAACATTGAAT
    AGACTTAACGGGGGATTCTTTGGACAAGTCTTTACTGTTTACTATAATGATAAGTATGTA
    GTTGTACTTGCATTAAATGTAAAAGGAAACAATGAAGTTCGAATCAAACATATTTATAAT
    GATATTTTAAAACAAAATAAACCTTACAATACGAAGGGTGTTATTGTTCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1194

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1066 [new locus tag: SACOL_RS05435 ]
  • symbol: SACOL1066
  • description: fmt protein
  • length: 397
  • theoretical pI: 10.1143
  • theoretical MW: 46067.1
  • GRAVY: -0.561713

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • FmtA protein involved in methicillin resistance
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Cell envelope-associated LytR-CpsA-Psr transcriptional attenuators  FmtA protein involved in methicillin resistance
  • PFAM:
    Beta-lactamase (CL0013) Beta-lactamase; Beta-lactamase (PF00144; HMM-score: 209.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 2
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.460433
    • TAT(Tat/SPI): 0.001127
    • LIPO(Sec/SPII): 0.024289
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETKLSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKMSKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFKTSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLVLAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRPNILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKKYPDEYRYGFYAKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTKGVIVQ

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

H Komatsuzawa, K Ohta, H Labischinski, M Sugai, H Suginaka
Characterization of fmtA, a gene that modulates the expression of methicillin resistance in Staphylococcus aureus.
Antimicrob Agents Chemother: 1999, 43(9);2121-5
[PubMed:10471551] [WorldCat.org] [DOI] (P p)H Komatsuzawa, M Sugai, K Ohta, T Fujiwara, S Nakashima, J Suzuki, C Y Lee, H Suginaka
Cloning and characterization of the fmt gene which affects the methicillin resistance level and autolysis in the presence of triton X-100 in methicillin-resistant Staphylococcus aureus.
Antimicrob Agents Chemother: 1997, 41(11);2355-61
[PubMed:9371333] [WorldCat.org] [DOI] (P p)

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