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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1183 [new locus tag: SACOL_RS06050 ]
  • pan locus tag?: SAUPAN003431000
  • symbol: SACOL1183
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1183 [new locus tag: SACOL_RS06050 ]
  • symbol: SACOL1183
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1187525..1189081
  • length: 1557
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    GTGGAAAATACAATTAATGAAAGTGAAAAGAAAAAACGATTTAAATTAAAAATGCCAGGT
    GCATTTATGATTTTATTCATTTTAACGGTTGTTGCAGTTATAGCAACATGGGTTATTCCT
    GCTGGTGCATATTCTAAACTTTCTTACGAACCTTCATCCCAAGAACTAAAGATAGTTAAC
    CCTCATAACCAAGTGAAAAAGGTTCCGGGTACGCAACAGGAACTAGACAAAATGGGGGTT
    AAAATTAAGATTGAACAATTTAAATCAGGTGCAATTAATAAGCCGGTATCAATTCCGAAT
    ACTTATGAAAGATTAAAGCAACATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTT
    GAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATT
    GGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACA
    AAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACA
    TTATGTGGTATTGAAGAAGAAGCTGTAGCATTTTATCCGATTTTAGTCCCTATATTTATA
    GCGTTAGGATACGATTCTATCGTTTCAGTTGGTGCCATATTCCTTGCCAGCTCTGTCGGT
    AGTACATTTTCAACTATTAACCCGTTCTCGGTTGTAATTGCCTCTAATGCCGCTGGTACA
    ACTTTTACGGATGGCTTGTATTGGAGAATAGGTGCTTGTATTGTCGGTGCGATTTTTGTT
    ATTAGTTATTTATATTGGTACTGTAAAAAAATTAAAAACGATCCTAAAGCGTCATATTCT
    TATGAAGACAAAGATGCTTTTGAACAGCAATGGTCTGTATTAAAAGATGACGATAGTGCC
    CATTTTACTTTGCGTAAGAAGATAATCCTTACATTATTTGTACTACCATTTCCAATTATG
    GTATGGGGAGTTATGACGCAAGGTTGGTGGTTCCCAGTTATGGCTTCAGCATTTTTAATA
    TTTACAATTATAATAATGTTTATTGCTGGGACAGGTAAATCTGGATTGGGAGAAAAAGGA
    ACTGTAGATGCATTTGTCAATGGTGCATCAAGTTTAGTAGGTGTATCTTTAATTATTGGT
    TTAGCTCGAGGTATTAATTTAGTGTTGAATGAAGGTATGATTTCAGATACAATCTTACAC
    TTTTCATCATCTTTAGTTCAACATATGAGTGGACCATTATTTATCATCGTATTACTATTT
    ATTTTCTTCTGTTTAGGTTTTATCGTGCCATCATCTTCTGGCTTAGCGGTATTATCAATG
    CCTATCTTTGCACCACTAGCTGATACAGTAGGTATACCAAGATTCGTCATCGTTACGACA
    TATCAATTTGGTCAATATGCGATGTTATTCTTAGCGCCGACTGGACTTGTTATGGCCACA
    CTACAAATGTTAAACATGCGATATTCACATTGGTTCCGATTTGTATGGCCGGTAGTTGCT
    TTTGTATTGATTTTCGGTGGCGGAGTACTAATTACGCAAGTACTAATTTATTCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1557

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1183 [new locus tag: SACOL_RS06050 ]
  • symbol: SACOL1183
  • description: hypothetical protein
  • length: 518
  • theoretical pI: 8.52842
  • theoretical MW: 56678.8
  • GRAVY: 0.666023

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids TRAP transporter, DctM subunit (TIGR00786; HMM-score: 29.5)
  • TheSEED  :
    • Arginine/ornithine antiporter ArcD
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine and Ornithine Degradation  Arginine/ornithine antiporter ArcD
    and 2 more
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Deiminase Pathway  Arginine/ornithine antiporter ArcD
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Polyamine Metabolism  Arginine/ornithine antiporter ArcD
  • PFAM:
    IT (CL0182) DcuC; C4-dicarboxylate anaerobic carrier (PF03606; HMM-score: 588.1)
    and 2 more
    DctM; Tripartite ATP-independent periplasmic transporter, DctM component (PF06808; HMM-score: 34.8)
    Na_H_antiporter; Na+/H+ antiporter family (PF03553; HMM-score: 18.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 43 aa
    • SP(Sec/SPI): 0.658728
    • TAT(Tat/SPI): 0.01712
    • LIPO(Sec/SPII): 0.005175
    • Cleavage Site: CS pos: 43-44. AGA-YS. Pr: 0.4478
  • predicted transmembrane helices (TMHMM): 13

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAYSKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTYERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKTKGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASSVGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPKASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVMASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEGMISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTVGIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGGVLITQVLIYS

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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