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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_1064 [new locus tag: SAUSA300_RS05765 ]
- pan locus tag?: SAUPAN003431000
- symbol: SAUSA300_1064
- pan gene symbol?: —
- synonym:
- product: TRAP family protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_1064 [new locus tag: SAUSA300_RS05765 ]
- symbol: SAUSA300_1064
- product: TRAP family protein
- replicon: chromosome
- strand: +
- coordinates: 1163706..1165262
- length: 1557
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3913443 NCBI
- RefSeq: YP_493762 NCBI
- BioCyc: see SAUSA300_RS05765
- MicrobesOnline: 1292579 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1501GTGGAAAATACAATTAATGAAAGTGAAAAGAAAAAACGATTTAAATTAAAAATGCCAGGT
GCATTTATGATTTTATTCATTTTAACGGTTGTTGCAGTTATAGCAACATGGGTTATTCCT
GCTGGTGCATATTCTAAACTTTCTTACGAACCTTCATCCCAAGAACTAAAGATAGTTAAC
CCTCATAACCAAGTGAAAAAGGTTCCGGGTACGCAACAGGAACTAGACAAAATGGGGGTT
AAAATTAAGATTGAACAATTTAAATCAGGTGCAATTAATAAGCCGGTATCAATTCCGAAT
ACTTATGAAAGATTAAAGCAACATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTT
GAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATT
GGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACA
AAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACA
TTATGTGGTATTGAAGAAGAAGCTGTAGCATTTTATCCGATTTTAGTCCCTATATTTATA
GCGTTAGGATACGATTCTATCGTTTCAGTTGGTGCCATATTCCTTGCCAGCTCTGTCGGT
AGTACATTTTCAACTATTAACCCGTTCTCGGTTGTAATTGCCTCTAATGCCGCTGGTACA
ACTTTTACGGATGGCTTGTATTGGAGAATAGGTGCTTGTATTGTCGGTGCGATTTTTGTT
ATTAGTTATTTATATTGGTACTGTAAAAAAATTAAAAACGATCCTAAAGCGTCATATTCT
TATGAAGACAAAGATGCTTTTGAACAGCAATGGTCTGTATTAAAAGATGACGATAGTGCC
CATTTTACTTTGCGTAAGAAGATAATCCTTACATTATTTGTACTACCATTTCCAATTATG
GTATGGGGAGTTATGACGCAAGGTTGGTGGTTCCCAGTTATGGCTTCAGCATTTTTAATA
TTTACAATTATAATAATGTTTATTGCTGGGACAGGTAAATCTGGATTGGGAGAAAAAGGA
ACTGTAGATGCATTTGTCAATGGTGCATCAAGTTTAGTAGGTGTATCTTTAATTATTGGT
TTAGCTCGAGGTATTAATTTAGTGTTGAATGAAGGTATGATTTCAGATACAATCTTACAC
TTTTCATCATCTTTAGTTCAACATATGAGTGGACCATTATTTATCATCGTATTACTATTT
ATTTTCTTCTGTTTAGGTTTTATCGTGCCATCATCTTCTGGCTTAGCGGTATTATCAATG
CCTATCTTTGCACCACTAGCTGATACAGTAGGTATACCAAGATTCGTCATCGTTACGACA
TATCAATTTGGTCAATATGCGATGTTATTCTTAGCGCCGACTGGACTTGTTATGGCCACA
CTACAAATGTTAAACATGCGATATTCACATTGGTTCCGATTTGTATGGCCGGTAGTTGCT
TTTGTATTGATTTTCGGTGGCGGAGTACTAATTACGCAAGTACTAATTTATTCATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_1064 [new locus tag: SAUSA300_RS05765 ]
- symbol: SAUSA300_1064
- description: TRAP family protein
- length: 518
- theoretical pI: 8.52842
- theoretical MW: 56678.8
- GRAVY: 0.666023
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Carbohydrates, organic alcohols, and acids TRAP transporter, DctM subunit (TIGR00786; HMM-score: 29.5)
- TheSEED :
- Arginine/ornithine antiporter ArcD
Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine and Ornithine Degradation Arginine/ornithine antiporter ArcDand 2 more - PFAM: IT (CL0182) DcuC; C4-dicarboxylate anaerobic carrier (PF03606; HMM-score: 588.1)and 2 moreDctM; Tripartite ATP-independent periplasmic transporter, DctM component (PF06808; HMM-score: 34.8)Na_H_antiporter; Na+/H+ antiporter family (PF03553; HMM-score: 18.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 12
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: 0.5
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: Signal peptide SP(Sec/SPI) length 43 aa
- SP(Sec/SPI): 0.658728
- TAT(Tat/SPI): 0.01712
- LIPO(Sec/SPII): 0.005175
- Cleavage Site: CS pos: 43-44. AGA-YS. Pr: 0.4478
- predicted transmembrane helices (TMHMM): 13
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAYSKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTYERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKTKGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASSVGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPKASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVMASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEGMISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTVGIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGGVLITQVLIYS
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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