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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

organism: Staphylococcus aureus COL
locus tag: SACOL1393 [new locus tag: SACOL_RS07090 ]
pan locus tag^?: SAUPAN003746000
symbol: SACOL1393
pan gene symbol^?: glcT
synonym:
product: transcription antiterminator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SACOL1393 [new locus tag: SACOL_RS07090 ]
symbol: SACOL1393
product: transcription antiterminator
replicon: chromosome
strand: +
coordinates: 1402582..1403433
length: 852
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Gene ID: 3236319 NCBI
RefSeq: YP_186245 NCBI
BioCyc: see SACOL_RS07090
MicrobesOnline: 912851 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

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ATGGGAGAATATATTGTTACTAAAACATTGAACAACAATGTCGTAGTATGTACTAATAAT
GATCAAGAAGTTATTTTAATCGGTAAAGGTATTGGTTTTAACAAAAAAGAGGGAATGGCG
TTAAACGACCAAACTATTACAATAGAGAAAATTTATAAATTAGAAAGTGAGCAACAAAAA
GCACATTATAAAAGTTTAGTTGAAATCGCTGATGATAATGTATTACAAGTAATTATTGAT
TCGTTGAATTTTATTTCTAATACTGCGATGAATGTTGATTCAAAACAACTTGTAGTTTCA
TTAACGGATCATATTATATTTGCTTATAAACGCTTAAAACAAAATCAAGTTATTAGCAAT
CCATTTGTTATGGAAACTATGCAGTTATATAGTGATGCATATCATATTGCTAAACAGGTG
ATTGATCAGTTAAATGCAGCATTAGATGTACATTTTCCTGAAGATGAGATAGGATTTATT
GCATTACATATTGCATCTAATACAGAAGATTTATCTATGCATGAGATGACCTTGATCAAT
AATGTTATTAAAAAAGGTATAGATATCATTGAATCAGACCTTGTGACAACTGTTGATAAG
GAATCATTACAATACCAACGTTTTATAAGGCACGTACAATTTTTAATTCGCCGATTAAGA
AGAAAAGAATATATACATGCACAAGATGATTTTGTGTCTATGATTAAAAATCACTATCCG
ATTTGCTATAACACAGCATATAAAATTTTAACTATGATACAAAAACAATTTGATGTTAAT
ATCAGTGAGTCTGAAATTATATATTTAACATTACACATTCATCATTTTGAAGAAAGGATT
AATCAATCCTAA
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⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SACOL1393 [new locus tag: SACOL_RS07090 ]
symbol: SACOL1393
description: transcription antiterminator
length: 283
theoretical pI: 5.9112
theoretical MW: 32822.4
GRAVY: -0.166431

⊟Function[edit | edit source]

TIGRFAM:
Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Riboflavin, FMN, and FAD GTP cyclohydrolase II (TIGR00505; EC 3.5.4.25; HMM-score: 12.4)
TheSEED :
- Beta-glucoside bgl operon antiterminator, BglG family
PFAM:
PRD (CL0166) PRD; PRD domain (PF00874; HMM-score: 142.3)
and 4 more
no clan defined CAT_RBD; CAT RNA binding domain (PF03123; HMM-score: 70)
DUF4956; Domain of unknown function (DUF4956) (PF16316; HMM-score: 13.9)
Dioxygenase_N; Catechol dioxygenase N terminus (PF04444; HMM-score: 12.9)
HTH (CL0123) FokI_C; Restriction endonuclease FokI, catalytic domain (PF02980; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004578
- TAT(Tat/SPI): 0.000184
- LIPO(Sec/SPII): 0.0008
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: 57650361 NCBI
RefSeq: YP_186245 NCBI
UniProt: Q5HG62 UniProt

⊟Protein sequence[edit | edit source]

MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQTITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLTDHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFIALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRRLRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHFEERINQS

⊟Experimental data[edit | edit source]

experimentally validated: PeptideAtlas
protein localization: Cytoplasmic ^[1] ^[2] ^[3]
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

MicrobesOnline: SACOL1393 > SACOL1394 > SACOL1395

⊟Regulation[edit | edit source]

regulator:

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e)
↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p)
↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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