From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1253 [new locus tag: SAUSA300_RS06805 ]
  • pan locus tag?: SAUPAN003746000
  • symbol: glcT
  • pan gene symbol?: glcT
  • synonym:
  • product: transcription antiterminator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1253 [new locus tag: SAUSA300_RS06805 ]
  • symbol: glcT
  • product: transcription antiterminator
  • replicon: chromosome
  • strand: +
  • coordinates: 1378770..1379630
  • length: 861
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    GTGATGATAATGGGAGAATATATTGTTACTAAAACATTGAACAACAATGTCGTAGTATGT
    ACTAATAATGATCAAGAAGTTATTTTAATCGGTAAAGGTATTGGTTTTAACAAAAAAGAG
    GGAATGGCGTTAAACGACCAAACTATTACAATAGAGAAAATTTATAAATTAGAAAGTGAG
    CAACAAAAAGCACATTATAAAAGTTTAGTTGAAATCGCTGATGATAATGTATTACAAGTA
    ATTATTGATTCGTTGAATTTTATTTCTAATACTGCGATGAATGTTGATTCAAAACAACTT
    GTAGTTTCATTAACGGATCATATTATATTTGCTTATAAACGCTTAAAACAAAATCAAGTT
    ATTAGCAATCCATTTGTTATGGAAACTATGCAGTTATATAGTGATGCATATCATATTGCT
    AAACAGGTGATTGATCAGTTAAATGCAGCATTAGATGTACATTTTCCTGAAGATGAGATA
    GGATTTATTGCATTACATATTGCATCTAATACAGAAGATTTATCTATGCATGAGATGACC
    TTGATCAATAATGTTATTAAAAAAGGTATAGATATCATTGAATCAGACCTTGTGACAACT
    GTTGATAAGGAATCATTACAATACCAACGTTTTATAAGGCACGTACAATTTTTAATTCGC
    CGATTAAGAAGAAAAGAATATATACATGCACAAGATGATTTTGTGTCTATGATTAAAAAT
    CACTATCCGATTTGCTATAACACAGCATATAAAATTTTAACTATGATACAAAAACAATTT
    GATGTTAATATCAGTGAGTCTGAAATTATATATTTAACATTACACATTCATCATTTTGAA
    GAAAGGATTAATCAATCCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    861

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1253 [new locus tag: SAUSA300_RS06805 ]
  • symbol: GlcT
  • description: transcription antiterminator
  • length: 286
  • theoretical pI: 5.9112
  • theoretical MW: 33198
  • GRAVY: -0.135664

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Riboflavin, FMN, and FAD GTP cyclohydrolase II (TIGR00505; EC 3.5.4.25; HMM-score: 12.3)
  • TheSEED  :
    • Beta-glucoside bgl operon antiterminator, BglG family
  • PFAM:
    PRD (CL0166) PRD; PRD domain (PF00874; HMM-score: 142.3)
    and 4 more
    no clan defined CAT_RBD; CAT RNA binding domain (PF03123; HMM-score: 70)
    DUF4956; Domain of unknown function (DUF4956) (PF16316; HMM-score: 13.8)
    Dioxygenase_N; Catechol dioxygenase N terminus (PF04444; HMM-score: 12.8)
    HTH (CL0123) FokI_C; Restriction endonuclease FokI, catalytic domain (PF02980; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007475
    • TAT(Tat/SPI): 0.000216
    • LIPO(Sec/SPII): 0.003051
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MMIMGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQTITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLTDHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFIALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRRLRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHFEERINQS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAUSA300_1253&oldid=72546"