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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1478 [new locus tag: SACOL_RS07530 ]
  • pan locus tag?: SAUPAN003893000
  • symbol: ald1
  • pan gene symbol?: ald1
  • synonym:
  • product: alanine dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1478 [new locus tag: SACOL_RS07530 ]
  • symbol: ald1
  • product: alanine dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 1515797..1516915
  • length: 1119
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGTTAGTTGCAGTAGTCAAAGAATTAAAACAAGGTGAAGGACGTGTAGCTTGCACACCC
    GAAAATGTGCGTAAGTTAACGGATGCAGGACATAAAGTAATTGTTGAAAAAAATGCTGGC
    ATTGGTTCAGGATTTTCTAACGATATGTATGAAAAAGAAGGCGCTAAGATCGTAACTCAC
    GAACAAGCATGGGAAGCTGATCTTGTTATCAAAGTAAAAGAACCTCATGAAAGCGAATAT
    CAATATTTCAAAAAGAATCAAATTATCTGGGGATTTTTACATCTAGCATCTTCAAAAGAA
    ATAGTAGAAAAAATGCAAGAAGTTGGTGTAACTGCGATTAGTGGTGAAACCATTATAAAA
    AATGGAAAAGCAGAATTATTAGCGCCAATGAGTGCTATAGCAGGTCAACGCTCAGCAATT
    ATGGGAGCTTACTACTCTGAAGCACAACATGGTGGTCAAGGTACTTTAGTGACTGGTGTA
    CATGAAAATGTGGATATACCTGGTAGTACATATGTGATTTTCGGTGGTGGAGTAGCAGCA
    ACAAATGCAGCAAATGTTGCCTTGGGACTAAATGCTAAAGTAATCATTATCGAGTTAAAC
    GATGACCGCATTAAATATCTTGAAGATATGTATGCAGAAAAAGATGTCACAGTAGTCAAA
    TCAACACCAGAAAATTTAGCAGAACAAATTAAGAAAGCAGATGTATTTATTTCTACAATT
    TTAATTCCAGGTGCGAAACCGCCAAAATTGGTTACTCGTGAGATGGTTAAATCAATGAAA
    AAAGGTTCAGTATTAATCGATATAGCTATTGACCAAGGTGGAACTATTGAAACAATTAGA
    CCAACTACAATTTCTGATCCAGTGTATGAAGAAGAAGGTGTGATTCATTATGGTGTACCA
    AATCAACCAGGAGCAGTCCCAAGAACTTCAACAATGGCATTAGCACAAGGAAATATTGAT
    TATATATTAGAAATTTGTGACAAAGGCTTAGAACAAGCAATTAAAGATAATGAAGCCTTA
    AGTACTGGTGTAAACATTTACCAAGGACAAGTGACAAATCAAGGATTAGCTTCATCACAT
    GACCTAGATTATAAAGAAATATTAAATGTTATCGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1119

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1478 [new locus tag: SACOL_RS07530 ]
  • symbol: Ald1
  • description: alanine dehydrogenase
  • length: 372
  • theoretical pI: 4.89305
  • theoretical MW: 40234.7
  • GRAVY: -0.140323

⊟Function[edit | edit source]

  • reaction:
    EC 1.4.1.1?  ExPASy
    Alanine dehydrogenase L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH
  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines alanine dehydrogenase (TIGR00518; EC 1.4.1.1; HMM-score: 395.5)
    and 6 more
    Metabolism Energy metabolism Electron transport NAD(P)(+) transhydrogenase (AB-specific), alpha subunit (TIGR00561; EC 1.6.1.2; HMM-score: 141.1)
    Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 23.2)
    Metabolism Energy metabolism Amino acids and amines adenosylhomocysteinase (TIGR00936; EC 3.3.1.1; HMM-score: 18.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamyl-tRNA reductase (TIGR01035; EC 1.2.1.70; HMM-score: 16.6)
    methylmalonyl-CoA mutase C-terminal domain (TIGR00640; HMM-score: 15.4)
    Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate-5-dehydrogenase (TIGR01809; EC 1.1.1.25; HMM-score: 15)
  • TheSEED  :
    • Alanine dehydrogenase (EC 1.4.1.1)
    Carbohydrates Central carbohydrate metabolism Pyruvate Alanine Serine Interconversions  Alanine dehydrogenase (EC 1.4.1.1)
  • PFAM:
    NADP_Rossmann (CL0063) AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 254.1)
    and 11 more
    Form_Glyc_dh (CL0325) AlaDh_PNT_N; Alanine dehydrogenase/PNT, N-terminal domain (PF05222; HMM-score: 142.1)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 25.8)
    THF_DHG_CYH_C; Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain (PF02882; HMM-score: 24.5)
    Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 22.7)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 20.3)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 17.2)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 14.9)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 14.2)
    ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 14.1)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 13.9)
    Form_Glyc_dh (CL0325) 2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 13.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011205
    • TAT(Tat/SPI): 0.000954
    • LIPO(Sec/SPII): 0.005196
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSGFSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIVEKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGVHENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTVVKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTIETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAIKDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Rex* (repression) regulon
    Rex*(TF)important in Energy metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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