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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1500 [new locus tag: SACOL_RS07640 ]
  • pan locus tag?: SAUPAN003920000
  • symbol: SACOL1500
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1500 [new locus tag: SACOL_RS07640 ]
  • symbol: SACOL1500
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1541508..1542215
  • length: 708
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    GTGAGTTTATTGTCTTCAATTTTTACTATGATAATTTATTTCGTAATATTAATGGTGCTT
    CCTTTATGGGCACAACACAAAGTTAAATCTAACTATGAGAAATATTCACAAGTTAGATCA
    ACTAGTGGTAAAACGGGTCGTGAAGTTGCATTAGAAATTTTACATGCAAACGGTATTTAT
    GATGTCGAAGTTGTTAAAGGTGAAGGATTCTTAACAGATCATTACGATCCTAAAAAGAAA
    GTTGTTTCACTATCTCCTGCAAACTATGACAGACCATCAGTTGCTGGAACTGCAATTGCA
    GCCCACGAAGTTGGTCATGCCATTCAAGATCACCAAGGATATTGGTTCTTAAGATTCAGA
    GCAGCATTAGTACCTGTTGCAAACCTAGGGAGTTCATTAAGTTATATGATCATCATGTTA
    GGTATTATCTTAACAGCGATAGGTAGTGCATTTGGTTCAACTGCTTTATGGATTGGTGCT
    GGTTTAATGTCATTAGCGGTATTATTCTCAATTGTTACGTTACCAGTTGAGTTTGATGCG
    AGTTCAAGAGCGATGAAACAAATTACGGCATTGAACATTGTAAATGAAAAAGAATACAAG
    CATGCGCGTAAAGTATTATCTGCAGCAGCAATGACTTATGTTGCAGCAACTGCAGTCGCT
    GTAGCAGAGCTTGTACGTATTATTTTGATTGCGCGTTCAAGTGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    708

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1500 [new locus tag: SACOL_RS07640 ]
  • symbol: SACOL1500
  • description: hypothetical protein
  • length: 235
  • theoretical pI: 9.59502
  • theoretical MW: 25561.7
  • GRAVY: 0.448936

⊟Function[edit | edit source]

  • TIGRFAM:
    membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family (TIGR03074; EC 1.1.-.-; HMM-score: 6.4)
  • TheSEED  :
    • probable metal-dependent peptidase
  • PFAM:
    Peptidase_MA (CL0126) Zn_peptidase_2; Putative neutral zinc metallopeptidase (PF04298; HMM-score: 295)
    and 2 more
    DUF4157; Domain of unknown function (DUF4157) (PF13699; HMM-score: 16.5)
    GPCR_A (CL0192) DUF3522; Protein of unknown function (DUF3522) (PF12036; HMM-score: 9.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 30 aa
    • SP(Sec/SPI): 0.627467
    • TAT(Tat/SPI): 0.00538
    • LIPO(Sec/SPII): 0.018787
    • Cleavage Site: CS pos: 30-31. VKS-NY. Pr: 0.2430
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSLLSSIFTMIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEILHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDHQGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLFSIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRIILIARSSD

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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