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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1526 [new locus tag: SACOL_RS07780 ]
  • pan locus tag?: SAUPAN003950000
  • symbol: SACOL1526
  • pan gene symbol?: ribU
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1526 [new locus tag: SACOL_RS07780 ]
  • symbol: SACOL1526
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1564750..1565295
  • length: 546
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGCAACAAAATAAACGTCTTATCACAATAAGTATGTTGAGCGCGATTGCGTTTGTGTTA
    ACTTTTATCAAGTTTCCTATACCATTTTTGCCACCATACTTAACTTTAGATTTTAGTGAT
    GTACCGTCACTACTAGCTACATTTACGTTTGGACCAGTTGCCGGTATCGTAGTTGCACTG
    GTTAAAAATTTATTGAACTACTTATTTAGTATGGGCGATCCAGTTGGACCATTTGCTAAC
    TTTTTAGCAGGCGCAAGTTTCTTATTAACTGCTTACGCCATCTATAAAAATAAACGTTCA
    ACAAAATCTTTGATTACTGGATTAATCATTGCAACAATCGTTATGACTATCGTGTTGAGT
    ATTTTGAACTATTTCGTTCTATTACCTTTGTACGGTATGATATTTAACTTAGCTGATATC
    GCAAATAATCTTAAAGTAATCATTGTTTCAGGAATTATACCATTCAATATTATTAAAGGT
    ATCGTTATTTCTATTGTATTTATTTTACTATATAGAAGGCTTGCGAATTTCTTGAAAAGA
    ATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    546

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL1526 [new locus tag: SACOL_RS07780 ]
  • symbol: SACOL1526
  • description: hypothetical protein
  • length: 181
  • theoretical pI: 10.6184
  • theoretical MW: 20024.3
  • GRAVY: 1.10552

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Unknown substrate ECF transporter S component, folate family (TIGR04518; HMM-score: 34.4)
    and 1 more
    membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family (TIGR03074; EC 1.1.-.-; HMM-score: 9.2)
  • TheSEED  :
    • Riboflavin ECF transporter, substrate-specific component RibU
    Membrane Transport Membrane Transport - no subcategory ECF class transporters  Substrate-specific component RibU of riboflavin ECF transporter
  • PFAM:
    Gx_transp (CL0315) ECF_trnsprt; ECF transporter, substrate-specific component (PF12822; HMM-score: 96.3)
    and 10 more
    ECF-ribofla_trS; ECF-type riboflavin transporter, S component (PF07155; HMM-score: 46.5)
    Thia_YuaJ; Thiamine transporter protein (Thia_YuaJ) (PF09515; HMM-score: 18.9)
    CbiM; Cobalt uptake substrate-specific transmembrane region (PF01891; HMM-score: 17.3)
    no clan defined DUF4118; Domain of unknown function (DUF4118) (PF13493; HMM-score: 15.6)
    Gx_transp (CL0315) Hpre_diP_synt_I; Heptaprenyl diphosphate synthase component I (PF07456; HMM-score: 12.4)
    no clan defined DUF1772; Domain of unknown function (DUF1772) (PF08592; HMM-score: 10)
    Tmemb_14; Transmembrane proteins 14C (PF03647; HMM-score: 8.7)
    DUF5083; Domain of unknown function (DUF5083) (PF16890; HMM-score: 7.4)
    MNHE; Na+/H+ ion antiporter subunit (PF01899; HMM-score: 6.5)
    Peroxisome (CL0484) Pex24p; Integral peroxisomal membrane peroxin (PF06398; HMM-score: 6.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.05587
    • TAT(Tat/SPI): 0.000847
    • LIPO(Sec/SPII): 0.010773
  • predicted transmembrane helices (TMHMM): 5

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSLLATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTKSLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKGIVISIVFILLYRRLANFLKRI

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: FMN-box (transcription termination) regulon
    FMN-box(RNA)important in Riboflavin biosynthesis; regulatory site identified based on RegPrecise data for N315 RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]