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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01505
  • pan locus tag?: SAUPAN003950000
  • symbol: SAOUHSC_01505
  • pan gene symbol?: ribU
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01505
  • symbol: SAOUHSC_01505
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1458085..1458630
  • length: 546
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGCAACAAAATAAACGTCTTATCACAATAAGTATGTTGAGCGCGATTGCGTTTGTGTTA
    ACTTTTATCAAGTTTCCTATACCATTTTTGCCACCATACTTAACTTTAGATTTTAGTGAT
    GTACCGTCACTACTAGCTACATTTACGTTTGGACCAGTTGCCGGTATCGTAGTTGCACTG
    GTTAAAAATTTATTGAACTACTTATTTAGTATGGGCGATCCAGTTGGACCATTTGCTAAC
    TTTTTAGCAGGCGCAAGTTTCTTATTAACTGCTTACGCCATCTATAAAAATAAACGTTCA
    ACAAAATCTTTGATTACTGGATTAATCATTGCAACAATCGTTATGACTATCGTGTTGAGT
    ATTTTGAACTATTTCGTTCTATTACCTTTGTACGGTATGATATTTAACTTAGCTGATATC
    GCAAATAATCTTAAAGTAATCATTGTTTCAGGAATTATACCATTCAATATTATTAAAGGT
    ATCGTTATTTCTATTGTATTTATTTTACTATATAGAAGGCTTGCGAATTTCTTGAAAAGA
    ATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    546

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01505
  • symbol: SAOUHSC_01505
  • description: hypothetical protein
  • length: 181
  • theoretical pI: 10.6184
  • theoretical MW: 20024.3
  • GRAVY: 1.10552

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Unknown substrate ECF transporter S component, folate family (TIGR04518; HMM-score: 34.4)
    and 1 more
    membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family (TIGR03074; EC 1.1.-.-; HMM-score: 9.2)
  • TheSEED  :
    • Riboflavin ECF transporter, substrate-specific component RibU
    Cofactors, Vitamins, Prosthetic Groups, Pigments Riboflavin, FMN, FAD Riboflavin, FMN and FAD metabolism Extended  Substrate-specific component RibU of riboflavin ECF transporter
    and 1 more
    Membrane Transport Membrane Transport - no subcategory ECF class transporters  Substrate-specific component RibU of riboflavin ECF transporter
  • PFAM:
    Gx_transp (CL0315) ECF_trnsprt; ECF transporter, substrate-specific component (PF12822; HMM-score: 96.3)
    and 10 more
    ECF-ribofla_trS; ECF-type riboflavin transporter, S component (PF07155; HMM-score: 46.5)
    Thia_YuaJ; Thiamine transporter protein (Thia_YuaJ) (PF09515; HMM-score: 18.9)
    CbiM; Cobalt uptake substrate-specific transmembrane region (PF01891; HMM-score: 17.3)
    no clan defined DUF4118; Domain of unknown function (DUF4118) (PF13493; HMM-score: 15.6)
    Gx_transp (CL0315) Hpre_diP_synt_I; Heptaprenyl diphosphate synthase component I (PF07456; HMM-score: 12.4)
    no clan defined DUF1772; Domain of unknown function (DUF1772) (PF08592; HMM-score: 10)
    Tmemb_14; Transmembrane proteins 14C (PF03647; HMM-score: 8.7)
    DUF5083; Domain of unknown function (DUF5083) (PF16890; HMM-score: 7.4)
    MNHE; Na+/H+ ion antiporter subunit (PF01899; HMM-score: 6.5)
    Peroxisome (CL0484) Pex24p; Integral peroxisomal membrane peroxin (PF06398; HMM-score: 6.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.05587
    • TAT(Tat/SPI): 0.000847
    • LIPO(Sec/SPII): 0.010773
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSLLATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTKSLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKGIVISIVFILLYRRLANFLKRI

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: FMN-box (transcription termination) regulon
    FMN-box(5' cis-acting region)important in Riboflavin biosynthesis; compare RegPrecise for N315 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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