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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL1658 [new locus tag: SACOL_RS08460 ]
- pan locus tag?: SAUPAN004195000
- symbol: SACOL1658
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL1658 [new locus tag: SACOL_RS08460 ]
- symbol: SACOL1658
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 1686496..1687716
- length: 1221
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3236440 NCBI
- RefSeq: YP_186498 NCBI
- BioCyc: see SACOL_RS08460
- MicrobesOnline: 913107 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1201ATGGGTTACAGAACTTTAAAAAGTATTTTTCATGAACACAATGAAAGTAAAATGAAGGAA
GAGTATATTAAAAGATTTAATTCTTTAGCTTCTTTCAATACTAATATTAATATCATACCT
ATGGAAAATGGAAAAAAAGTTAATGATTTGGAATATCCTCTATTCTTTATGGTGACTAAA
AATCTATCAAAAAAACAAGAATTAATATCAATTAATAGTAGAAAAATTGATAGAGCACTT
AATTCTTTACCATATGCAGCTAGAGAACAATATTTTAATGATTTATTAATCGATGAATTA
CAAAGTACTAATGAAATTGAAAATGTATTTAGTACTAAACAAGAGATTGCACATGCGTTA
AATAACCAAGCATCAGAATTTCTTAAGTTCAGAGGCCTCGTGGATCAATATAAAGAGATA
GAACTTAATAAAAAAATTAAAGTTGATAATGTAAGAGACATTAGAGCGATTTATGATAAA
TTAGTTTCAAATGAAATTAACGAACAAGATAAGTTAGATGGAGAGCTATTTCGTAAAAAT
TTTGTCGGTGTGCATGATGGGTCAACGAATAAATATATACATGTTGGGTTACAACCTGAA
ACCAAAATTGTTGAATTTATAGGTGAAATGCTAACATTTTTAAAATATTTTGATGCGCCT
CAGCCGTTCAAAATCATGGCTAGTCATTATCTGTTTGAATATATACATCCTTTTTATGAT
GGCAATGGTAGAGTTGGAAGATTTATAATTGCCAAGCTCTTGAGTGATTATTATGATAAT
TATACTGCTTTGACATTTTCATATGTTATAAATAGAAATAAATCGAAATATTACAAGGCG
TTTATGACTGCTTCAAATCATTTGAATTGTGGTGATTTGACGGAATTTATTGACACAATG
CTTGAGCTATTGATAGCAGGACAAGAAAGAATACTTGATGAGTTAATACCAAAAATGGAT
GCGACTGAAAAATTAACGCTTTATTTAACTAGTCATTATAAACAGATAGATTATGAATTT
TTGTATCTCCTTTCAATGGATAAATTGTTTGGAAATAAAAGAAATAGATTAACATTAATT
GATTTAGAAAACATATTGGGAGTAGGAAGAGTAAAAATTAATAATACAATTAAAAAATAT
GATAACTACTTAGTTAAAATTAAAAGTAGACCAACAATTTATGAGATAAGTGATGAATTT
CTAAATTCGATTATAAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL1658 [new locus tag: SACOL_RS08460 ]
- symbol: SACOL1658
- description: hypothetical protein
- length: 406
- theoretical pI: 7.78584
- theoretical MW: 47684.4
- GRAVY: -0.398522
⊟Function[edit | edit source]
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.005067
- TAT(Tat/SPI): 0.000494
- LIPO(Sec/SPII): 0.000922
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MGYRTLKSIFHEHNESKMKEEYIKRFNSLASFNTNINIIPMENGKKVNDLEYPLFFMVTKNLSKKQELISINSRKIDRALNSLPYAAREQYFNDLLIDELQSTNEIENVFSTKQEIAHALNNQASEFLKFRGLVDQYKEIELNKKIKVDNVRDIRAIYDKLVSNEINEQDKLDGELFRKNFVGVHDGSTNKYIHVGLQPETKIVEFIGEMLTFLKYFDAPQPFKIMASHYLFEYIHPFYDGNGRVGRFIIAKLLSDYYDNYTALTFSYVINRNKSKYYKAFMTASNHLNCGDLTEFIDTMLELLIAGQERILDELIPKMDATEKLTLYLTSHYKQIDYEFLYLLSMDKLFGNKRNRLTLIDLENILGVGRVKINNTIKKYDNYLVKIKSRPTIYEISDEFLNSIIK
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Cytoplasmic [1] [2]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)