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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1832 [new locus tag: SACOL_RS09395 ]
  • pan locus tag?: SAUPAN004482000
  • symbol: crcB
  • pan gene symbol?: crcB1
  • synonym:
  • product: camphor resistance protein CrcB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1832 [new locus tag: SACOL_RS09395 ]
  • symbol: crcB
  • product: camphor resistance protein CrcB
  • replicon: chromosome
  • strand: +
  • coordinates: 1886613..1887056
  • length: 444
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGCATCGACAATTTTTGTCGTCGCGTTGCCAAAACCTCTTTTTTAAATTCAAACTACTT
    CTTTTCGAGGTGAACCAAATGCAATATGTATATATTTTTATCGGTGGTGCTTTAGGCGCT
    TTATTACGTTACCTCATTTCTTTTCTGAATACTGACGGAGGTTTTCCAATCGGAACACTG
    ATAGCCAATTTGACTGGTGCCTTTGTAATGGGATTGCTAACAGCCTTAACAATTGCATTT
    TTTTCAAACCATCCGACCCTAAAAAAAGCTATTACGACTGGTTTTCTTGGTGCTTTAACG
    ACTTTTTCAACATTTCAATTAGAATTAATACATATGTTTGATCATCAACAATTTATAACT
    TTACTACTATATGCTGTAACAAGTTATGTCTTTGGTATTTTGTTATGTTACGTCGGTATA
    AAACTAGGTGGTGGTTTATCATGA
    60
    120
    180
    240
    300
    360
    420
    444

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1832 [new locus tag: SACOL_RS09395 ]
  • symbol: CrcB
  • description: camphor resistance protein CrcB
  • length: 147
  • theoretical pI: 9.45493
  • theoretical MW: 16352.3
  • GRAVY: 0.817687

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function General protein CrcB (TIGR00494; HMM-score: 84.1)
  • TheSEED  :
    • Fluoride ion exporter CrcB/FEX
  • PFAM:
    DMT (CL0184) CRCB; CrcB-like protein, Camphor Resistance (CrcB) (PF02537; HMM-score: 91.3)
    and 5 more
    UMP_1 (CL0470) ThrE_2; Threonine/Serine exporter, ThrE (PF12821; HMM-score: 17.2)
    Holin-III (CL0564) Phage_holin_3_1; Phage holin family (Lysis protein S) (PF05106; HMM-score: 11.3)
    Phage_holin_3_3; LydA holin phage, holin superfamily III (PF16083; HMM-score: 11.3)
    no clan defined CbtA; Probable cobalt transporter subunit (CbtA) (PF09490; HMM-score: 7.1)
    DUF2070; Predicted membrane protein (DUF2070) (PF09843; HMM-score: 7.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003057
    • TAT(Tat/SPI): 0.000175
    • LIPO(Sec/SPII): 0.001041
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MHRQFLSSRCQNLFFKFKLLLFEVNQMQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVMGLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTSYVFGILLCYVGIKLGGGLS

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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