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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1859 [new locus tag: SACOL_RS09535 ]
  • pan locus tag?: SAUPAN004553000
  • symbol: SACOL1859
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1859 [new locus tag: SACOL_RS09535 ]
  • symbol: SACOL1859
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1909269..1912319
  • length: 3051
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGAAGAAGGTTATCGATTAAAGTTTATATTTATTGGAAATCAAAATAATAATATTAAA
    AATAAGAATTTTTCAAATCCACATAATATATTATTCGATAGTAAAAAAGATATTATTTTA
    ACTCAAGACTTATGCGAAGAGTTTTTGAATTTAAACATAAATAAGCAAGATCACGCTATT
    GAATTATTAAAAAAAGAATTATCTCCGCTTTTATTTGAAGATTCTTTGTCATATTTAAAA
    GAAGAATTTATTAATGAAAAATTAGAATTTAATATATCGAATTTAGCTTCACGATATACT
    GCGAATAATGATGTCGATACTATTAATAATAAAATTATAGAAGGTATTTCTATTACCAAT
    AATTTTAAATATACTAATATTTCATATCTAAAAGAATTAAAAGGTTATATCGAAAATGAT
    ATTTTAGATAAGATGAAATCAAAATACGCAAAGAATATTTATTTGAATTTCAAAAAAATA
    TTCTCTAATTTAGAACAATCAGTCAATAATTATTTGGAACTGGAAGAAGAGTTTGAAGAA
    AAGAAAAAATACTTAAGTGAAATTTATGAATTGATAGACGAAATTAATATTGACCCTTAT
    ATTTTTCTGACAGAACATAATGAATGTAATATATACAAAATAAGTGAGAATGAAAAATTA
    GAACTTCAAACTTATATGAGTAAAATCGAAAAAGTATTATTAAAATATCAAACGTATTTA
    AAAGAAACATGTAAAGAATGCTTGTTTTATCCTTATTTGCTAGTTCAAGGTGAAGCTGGC
    ATAGGAAAATCACACCTTTTAGCGCATCTATCTAAAAAACTTAGAGATGAAAATCATATT
    ATTTATCTGTTTTTGGGACAATTTTTTACTAAAAATGAAGATCCATGGCATCAAATACTT
    AATGATTTAGAAGTTACAAATTCTGTTGATAATTTTTTAAGGTCAATAAGTAATAAGGCC
    AAAGAAACAAAAAAAAGAGCTTTTATTATTATTGATGCGCTTAATGAAGGTGAAGGTAAA
    AGGTTATGGGGAAATTATTTTCAAAGCTTTATCAACCATATAAAGAAGTATTCTAATATA
    GCTTTAATATTTTCTATTAGAACCCCTTTTGAAGATGTTATATTACCCAAAAACGCAATA
    CAAGATAACAATATTGTAGTATTTCAGCATGAAGGTTTTAGTAAGGAAGAAAACTATAAT
    CCAATTGTATCTTTTTGTGATTTTTATGGATTAGAGCTACCTAAGTTACCTATATTAAAT
    CCAGAATTCAACAATCCATTATTTTTAAAACTAATGTGTGAGTACTGTGTAAATAAATTT
    AAGGAATTTGACCAAACTATAAGTGTTGCAGAGCTATTTACAAATGTTCTTAAGACAGTA
    AATATTAATTTATCTAAGGAAGATAAATTTGATTTTGATAAAAATATTAATGTTGTACAA
    AAAGTAATAAAAGGATTAGTTGAATTAATGAACGACTCAGAATTTAATCAACTAAATTAT
    GAGGAATCCTATACTGTTGTTAATAATATAGCTAAAGAGTATGTTCAAAAGTCAAATAGA
    TTTTTAGAAGCGCTAATAGATGAAAATATATTAATTAAAAATACTGGCTATAAAGGTGAA
    ATGATTATTTATTTTTCGTATGAGCGTATGGGAGATTACTTTTTATCAGAATACCTTTTA
    GAGAAATATAGAAATGTTGATAAAAGAGATTTAGTAACTAAATTACAGTCAGATGAAAAA
    GTGACAAGGTATTTTCAAAAGGAAGACGATTTATCTTATAATAGAGGTCTTATTAATGAA
    CTTTTTATTAAATTAGCAAATGAATTTAATATAGAGCTATTTGAAGTATTTCCACAGTTT
    AAAAACAATTATAACATGATATATTCTTTTATAAATAGTTTAGTATGGAGAAAAGATGGT
    AGCATAAGTAAACATACTAAATGCTATATATCCGATAATGTTATTCCTTATGATGCATTT
    AGAAATAATTTTTTAGATGTCTTATTAATAAAAATGCCTCAAAAAAACCATCCGTTAAAT
    ATTTGGGCTTTACATAAATTATTGAAACAATGCAATTTAGGAAAAAGAGATTTTTTATGG
    ACTCAATATATATCTATAAATAATGAAAAGGTTTTTGAAATTATTAATTGGCTATTTAGT
    AACTATAAGAAATTAGATGAAGAAACTGCGGAAAAATATATGATTTTTTTAACGTGGATA
    TTTTCTGCAACTAATAATAAATTAAGAGATCTCGGTACTAAATCTTTAGTAAAATTATTC
    AAAACATTTCCAACTAAAATTATTGGTTTATTAAAATTATTTGAAAATAACAATGATCCA
    TATATAGTCGAAAGATTATATGCTTCTGTTTTAGGGGCGACACTTCGAATCGACATTTGT
    GAAATACATATAGAGATTGCTAATTATATTTATGAAGAAATATTTGATAAAGAAATGGTA
    TATCCTCATATTTTAATGAGAGATTATGCTAGGCAAACAATTGAATATATTAGTTTATCA
    AAAGATATATCTAATATAAATTTAGAAAAAATTAGGCCACCTTATAAAAGTAATTGGTAT
    AAAAAAGAATATTCGAATTTAAATATAGACGATTACATAAAATCTTTAAAAAACAAATTA
    GATAGCCATTTACATTTTTCTATCGATAAAATTAAAAACTCAATGACAACGGAATATGGA
    AGAGGAACTGGGGCATATGGCGATTTTGGCAGATATGTATTTGGTTATGCTGTACGAAAC
    TGGGTAAAGGGTTTTAAAAGTGATCAAGACTTAAGTAATATAGCATTAATGAGGATTTTT
    GAAATGGGTTATGATGCAAAATTACATGGTGAATTTGATATGTGGGTTAATCGTTATGAT
    AACTTTAATAATTCAATTGAAAGAATTAGCAAAAAATATCAATGGATTGCTTACTATGAA
    ATTTTAGCTAAACTTGTAGATAAATTTCCTGATGTTCAATATTCTGGACTATGGGATGAT
    TATATTAGAGATATTGATCCTACTTTACTATTACTTGAAATTGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3051

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL1859 [new locus tag: SACOL_RS09535 ]
  • symbol: SACOL1859
  • description: hypothetical protein
  • length: 1016
  • theoretical pI: 5.76406
  • theoretical MW: 120680
  • GRAVY: -0.414665

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaA regulatory inactivator Hda (TIGR03420; HMM-score: 21.1)
    and 1 more
    Cellular processes Cellular processes Toxin production and resistance TOMM system kinase/cyclase fusion protein (TIGR03903; HMM-score: 10.4)
  • TheSEED  :
    • [Genomic island nu Sa beta2]
  • PFAM:
    P-loop_NTPase (CL0023) AAA_16; AAA ATPase domain (PF13191; HMM-score: 22.4)
    NACHT; NACHT domain (PF05729; HMM-score: 22.2)
    AAA_22; AAA domain (PF13401; HMM-score: 20.5)
    and 4 more
    RNA_helicase; RNA helicase (PF00910; HMM-score: 13.7)
    ABC_tran; ABC transporter (PF00005; HMM-score: 13.1)
    no clan defined ANT; Phage antirepressor protein KilAC domain (PF03374; HMM-score: 12.8)
    DUF1284; Protein of unknown function (DUF1284) (PF06935; HMM-score: 8.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002336
    • TAT(Tat/SPI): 0.000072
    • LIPO(Sec/SPII): 0.000302
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MEEGYRLKFIFIGNQNNNIKNKNFSNPHNILFDSKKDIILTQDLCEEFLNLNINKQDHAIELLKKELSPLLFEDSLSYLKEEFINEKLEFNISNLASRYTANNDVDTINNKIIEGISITNNFKYTNISYLKELKGYIENDILDKMKSKYAKNIYLNFKKIFSNLEQSVNNYLELEEEFEEKKKYLSEIYELIDEINIDPYIFLTEHNECNIYKISENEKLELQTYMSKIEKVLLKYQTYLKETCKECLFYPYLLVQGEAGIGKSHLLAHLSKKLRDENHIIYLFLGQFFTKNEDPWHQILNDLEVTNSVDNFLRSISNKAKETKKRAFIIIDALNEGEGKRLWGNYFQSFINHIKKYSNIALIFSIRTPFEDVILPKNAIQDNNIVVFQHEGFSKEENYNPIVSFCDFYGLELPKLPILNPEFNNPLFLKLMCEYCVNKFKEFDQTISVAELFTNVLKTVNINLSKEDKFDFDKNINVVQKVIKGLVELMNDSEFNQLNYEESYTVVNNIAKEYVQKSNRFLEALIDENILIKNTGYKGEMIIYFSYERMGDYFLSEYLLEKYRNVDKRDLVTKLQSDEKVTRYFQKEDDLSYNRGLINELFIKLANEFNIELFEVFPQFKNNYNMIYSFINSLVWRKDGSISKHTKCYISDNVIPYDAFRNNFLDVLLIKMPQKNHPLNIWALHKLLKQCNLGKRDFLWTQYISINNEKVFEIINWLFSNYKKLDEETAEKYMIFLTWIFSATNNKLRDLGTKSLVKLFKTFPTKIIGLLKLFENNNDPYIVERLYASVLGATLRIDICEIHIEIANYIYEEIFDKEMVYPHILMRDYARQTIEYISLSKDISNINLEKIRPPYKSNWYKKEYSNLNIDDYIKSLKNKLDSHLHFSIDKIKNSMTTEYGRGTGAYGDFGRYVFGYAVRNWVKGFKSDQDLSNIALMRIFEMGYDAKLHGEFDMWVNRYDNFNNSIERISKKYQWIAYYEILAKLVDKFPDVQYSGLWDDYIRDIDPTLLLLEIDK

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]