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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1750 [new locus tag: SAUSA300_RS09575 ]
  • pan locus tag?: SAUPAN004553000
  • symbol: SAUSA300_1750
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1750 [new locus tag: SAUSA300_RS09575 ]
  • symbol: SAUSA300_1750
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1930682..1934794
  • length: 4113
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    TTGAAACGATCAACGAATCAAGAAAAATTTCTTGATACACTAATTAGACTTAATACTAAG
    ATTGAAGAACTAGGAAAGATAAATATTTTAAATAATCATATATATTCCGAATATTTTTTT
    AGAGACCTGTTAAATATTGTATATGGGTATTCACTTGAGAATCATAATAAAAAACAAAAA
    AATGCACCAGCATTTGATCTTATAGATAATACGAATAAAATTATTATTCAAGTTACCGCA
    ACGTGTAAAAAGCAAAAAATAGAGGACACTCTAAAAAAAGAGTATTTAACAAATAAAATG
    GAAGAAGGTTATCGATTAAAGTTTATATTTATTGGAAATCAAAATAATAATATTAAAAAT
    AAGAATTTTTCAAATCCACATAATATATTATTCGATAGTAAAAAAGATATTATTTTAACT
    CAAGACTTATGCGAAGAGTTTTTGAATTTAAACATAAATAAGCAAGATCACGCTATTGAA
    TTATTAAAAAAAGAATTATCTCCGCTTTTATTTGAAGATTCTTTGTCATATTTAAAAGAA
    GAATTTATTAATGAAAAATTAGAATTTAATATATCGAATTTAGCTTCACGATATACTGCG
    AATAATGATGTCGATACTATTAATAATAAAATTATAGAAGGTATTTCTATTACCAATAAT
    TTTAAATATACTAATATTTCATATCTAAAAGAATTAAAAGGTTATATCGAAAATGATATT
    TTAGATAAGATGAAATCAAAATACGCAAAGAATATTTATTTGAATTTCAAAAAAATATTC
    TCTAATTTAGAACAATCAGTCAATAATTATTTGGAACTGGAAGAAGAGTTTGAAGAAAAG
    AAAAAATACTTAAGTGAAATTTATGAATTGATAGACGAAATTAATATTGACCCTTATATT
    TTTCTGACAGAACATAATGAATGTAATATATACAAAATAAGTGAGAATGAAAAATTAGAA
    CTTCAAACTTATATGAGTAAAATCGAAAAAGTATTATTAAAATATCAAACGTATTTAAAA
    GAAACATGTAAAGAATGCTTGTTTTATCCTTATTTGCTAGTTCAAGGTGAAGCTGGCATA
    GGAAAATCACACCTTTTAGCGCATCTATCTAAAAAACTTAGAGATGAAAATCATATTATT
    TATCTGTTTTTGGGACAATTTTTTACTAAAAATGAAGATCCATGGCATCAAATACTTAAT
    GATTTAGAAGTTACAAATTCTGTTGATAATTTTTTAAGGTCAATAAGTAATAAGGCCAAA
    GAAACAAAAAAAAGAGCTTTTATTATTATTGATGCGCTTAATGAAGGTGAAGGTAAAAGG
    TTATGGGGAAATTATTTTCAAAGCTTTATCAACCATATAAAGAAGTATTCTAATATAGCT
    TTAATATTTTCTATTAGAACCCCTTTTGAAGATGTTATATTACCCAAAAACGCAATACAA
    GATAACAATATTGTAGTATTTCAGCATGAAGGTTTTAGTAAGGAAGAAAACTATAATCCA
    ATTGTATCTTTTTGTGATTTTTATGGATTAGAGCTACCTAAGTTACCTATATTAAATCCA
    GAATTCAACAATCCATTATTTTTAAAACTAATGTGTGAGTACTGTGTAAATAAATTTAAG
    GAATTTGACCAAACTATAAGTGTTGCAGAGCTATTTACAAATGTTCTTAAGACAGTAAAT
    ATTAATTTATCTAAGGAAGATAAATTTGATTTTGATAAAAATATTAATGTTGTACAAAAA
    GTAATAAAAGGATTAGTTGAATTAATGAACGACTCAGAATTTAATCAACTAAATTATGAG
    GAATCCTATACTGTTGTTAATAATATAGCTAAAGAGTATGTTCAAAAGTCAAATAGATTT
    TTAGAAGCGCTAATAGATGAAAATATATTAATTAAAAATACTGGCTATAAAGGTGAAATG
    ATTATTTATTTTTCGTATGAGCGTATGGGAGATTACTTTTTATCAGAATACCTTTTAGAG
    AAATATAGAAATGTTGATAAAAGAGATTTAGTAACTAAATTACAGTCAGATGAAAAAGTG
    ACAAGGTATTTTCAAAAGGAAGACGATTTATCTTATAATAGAGGTCTTATTAATGAACTT
    TTTATTAAATTAGCAAATGAATTTAATATAGAGCTATTTGAAGTATTTCCACAGTTTAAA
    AACAATTATAACATGATATATTCTTTTATAAATAGTTTAGTATGGAGAAAAGATGGTAGC
    ATAAGTAAACATACTAAATGCTATATATCCGATAATGTTATTCCTTATGATGCATTTAGA
    AATAATTTTTTAGATGTCTTATTAATAAAAATGCCTCAAAAAAACCATCCGTTAAATATT
    TGGGCTTTACATAAATTATTGAAACAATGCAATTTAGGAAAAAGAGATTTTTTATGGACT
    CAATATATATCTATAAATAATGAAAAGGTTTTTGAAATTATTAATTGGCTATTTAGTAAC
    TATAAGAAATTAGATGAAGAAACTGCGGAAAAATATATGATTTTTTTAACGTGGATATTT
    TCTGCAACTAATAATAAATTAAGAGATCTCGGTACTAAATCTTTAGTAAAATTATTCAAA
    ACATTTCCAACTAAAATTATTGGTTTATTAAAATTATTTGAAAATAACAATGATCCATAT
    ATAGTCGAAAGATTATATGCTTCTGTTTTAGGGGCGACACTTCGAATCGACATTTGTGAA
    ATACATATAGAGATTGCTAATTATATTTATGAAGAAATATTTGATAAAGAAATGGTATAT
    CCTCATATTTTAATGAGAGATTATGCTAGGCAAACAATTGAATATATTAGTTTATCAAAA
    GATATATCTAATATAAATTTAGAAAAAATTAGGCCACCTTATAAAAGTAATTGGTATAAA
    AAAGAATATTCGAATTTAAATATAGACGATTACATAAAATCTTTAAAAAACAAATTAGAT
    AGCCATTTACATTTTTCTATCGATAAAATTAAAAACTCAATGACAACGGAATATGGAAGA
    GGAACTGGGGCATATGGCGATTTTGGCAGATATGTATTTGGTTATGCTGTACGAAACTGG
    GTAAAGGGTTTTAAAAGTGATCAAGACTTAAGTAATATAGCATTAATGAGGATTTTTGAA
    ATGGGTTATGATGCAAAATTACATGGTGAATTTGATATGTGGGTTAATCGTTATGATAAC
    TTTAATAATTCAATTGAAAGAATTAGCAAAAAATATCAATGGATTGCTTACTATGAAATT
    TTAGCTAAACTTGTAGATAAATTTCCTGATGTTCAATATTCTGGACTATGGGATGATTAT
    ATTAGAGATATTGATCCTACTTTACTATTACTTGAAATTGATAAAGAATCAAAAATACTA
    GTACCCTCACCACTTCCTTCGCATCAATCGAATGAATGGGTAAAAAATACCAAAGTTTTT
    GATGAAACAAAATTATTTTTAGAAATAGATATAGACAATCATAGATATATATGTTTAAGC
    TCAAAGTTTAATTTTGAAAAGAGAGAGAAAGAAATACCATTTGAAGATAGAGACTCATGT
    TATTTCTTAGCGATGGGATATTTTTATAATAAAGAAGATAGTAATGAAATTATCAAAGGT
    TATGAAAATAATTATGATAGAGGTATTAATATTCCTCGAGCACATAGTATATATTTATAT
    GAATATTATTGGAGCGAAGCTTATAAAAATTATAAAGAGGGATATTTAACTGAATCAGAT
    GGTAAATTATGTCCAGCTATTTATGAATATTTTTGGGAGTTAGATTATTCTGTTAAGGAC
    AAATCAATAAGTTTTTATATTCCGTGTAAAGAAATAGTAGATTATTTTTCATTAATACAA
    ACGGAAGAAGGAGTATGGAAAACTAAATTTGGTGAAACAATATGTATTAATTCTAAATTA
    CTCGAATTTGATAATGAATGTTTACTTATTAAAAAAGAAAGTTTATTAAACTTTCTTAAT
    ACTAAAAAATTGTCTATTGGATGGAAAATATATCTAGAAAAAATTTCTCTTCGAGATAGA
    CAAGAATGGTGGTACAATGTTTTTTATGACGATGGCAAATATAATAAGAAAATAATTAAG
    AATGACATGTCAAAAATTAGGCGTAATTTTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3720
    3780
    3840
    3900
    3960
    4020
    4080
    4113

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1750 [new locus tag: SAUSA300_RS09575 ]
  • symbol: SAUSA300_1750
  • description: hypothetical protein
  • length: 1370
  • theoretical pI: 6.19337
  • theoretical MW: 163266
  • GRAVY: -0.481095

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaA regulatory inactivator Hda (TIGR03420; HMM-score: 20.1)
    and 1 more
    Cellular processes Cellular processes Toxin production and resistance TOMM system kinase/cyclase fusion protein (TIGR03903; HMM-score: 9.9)
  • TheSEED  :
    • [Genomic island nu Sa beta2]
  • PFAM:
    P-loop_NTPase (CL0023) NACHT; NACHT domain (PF05729; HMM-score: 21.8)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 21.7)
    AAA_22; AAA domain (PF13401; HMM-score: 20)
    and 3 more
    RNA_helicase; RNA helicase (PF00910; HMM-score: 15.9)
    no clan defined ANT; Phage antirepressor protein KilAC domain (PF03374; HMM-score: 12.6)
    P-loop_NTPase (CL0023) ABC_tran; ABC transporter (PF00005; HMM-score: 12.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002121
    • TAT(Tat/SPI): 0.00021
    • LIPO(Sec/SPII): 0.000337
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKRSTNQEKFLDTLIRLNTKIEELGKINILNNHIYSEYFFRDLLNIVYGYSLENHNKKQKNAPAFDLIDNTNKIIIQVTATCKKQKIEDTLKKEYLTNKMEEGYRLKFIFIGNQNNNIKNKNFSNPHNILFDSKKDIILTQDLCEEFLNLNINKQDHAIELLKKELSPLLFEDSLSYLKEEFINEKLEFNISNLASRYTANNDVDTINNKIIEGISITNNFKYTNISYLKELKGYIENDILDKMKSKYAKNIYLNFKKIFSNLEQSVNNYLELEEEFEEKKKYLSEIYELIDEINIDPYIFLTEHNECNIYKISENEKLELQTYMSKIEKVLLKYQTYLKETCKECLFYPYLLVQGEAGIGKSHLLAHLSKKLRDENHIIYLFLGQFFTKNEDPWHQILNDLEVTNSVDNFLRSISNKAKETKKRAFIIIDALNEGEGKRLWGNYFQSFINHIKKYSNIALIFSIRTPFEDVILPKNAIQDNNIVVFQHEGFSKEENYNPIVSFCDFYGLELPKLPILNPEFNNPLFLKLMCEYCVNKFKEFDQTISVAELFTNVLKTVNINLSKEDKFDFDKNINVVQKVIKGLVELMNDSEFNQLNYEESYTVVNNIAKEYVQKSNRFLEALIDENILIKNTGYKGEMIIYFSYERMGDYFLSEYLLEKYRNVDKRDLVTKLQSDEKVTRYFQKEDDLSYNRGLINELFIKLANEFNIELFEVFPQFKNNYNMIYSFINSLVWRKDGSISKHTKCYISDNVIPYDAFRNNFLDVLLIKMPQKNHPLNIWALHKLLKQCNLGKRDFLWTQYISINNEKVFEIINWLFSNYKKLDEETAEKYMIFLTWIFSATNNKLRDLGTKSLVKLFKTFPTKIIGLLKLFENNNDPYIVERLYASVLGATLRIDICEIHIEIANYIYEEIFDKEMVYPHILMRDYARQTIEYISLSKDISNINLEKIRPPYKSNWYKKEYSNLNIDDYIKSLKNKLDSHLHFSIDKIKNSMTTEYGRGTGAYGDFGRYVFGYAVRNWVKGFKSDQDLSNIALMRIFEMGYDAKLHGEFDMWVNRYDNFNNSIERISKKYQWIAYYEILAKLVDKFPDVQYSGLWDDYIRDIDPTLLLLEIDKESKILVPSPLPSHQSNEWVKNTKVFDETKLFLEIDIDNHRYICLSSKFNFEKREKEIPFEDRDSCYFLAMGYFYNKEDSNEIIKGYENNYDRGINIPRAHSIYLYEYYWSEAYKNYKEGYLTESDGKLCPAIYEYFWELDYSVKDKSISFYIPCKEIVDYFSLIQTEEGVWKTKFGETICINSKLLEFDNECLLIKKESLLNFLNTKKLSIGWKIYLEKISLRDRQEWWYNVFYDDGKYNKKIIKNDMSKIRRNF

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]