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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL1880 [new locus tag: SACOL_RS09650 ]
- pan locus tag?: SAUPAN004583000
- symbol: lukD
- pan gene symbol?: lukD
- synonym:
- product: leukotoxin LukD
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL1880 [new locus tag: SACOL_RS09650 ]
- symbol: lukD
- product: leukotoxin LukD
- replicon: chromosome
- strand: -
- coordinates: 1934671..1935654
- length: 984
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3236709 NCBI
- RefSeq: YP_186707 NCBI
- BioCyc: see SACOL_RS09650
- MicrobesOnline: 913322 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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961ATGAAAATGAAAAAATTAGTCAAATCATCAGTTGCTTCATCAATTGCACTGCTTTTGCTA
TCGAATACAGTTGATGCAGCTCAACATATCACACCTGTAAGCGAGAAAAAAGTAGATGAC
AAAATCACTTTATACAAAACAACAGCAACATCTGATAATGATAAATTGAATATTTCTCAA
ATTTTAACGTTTAATTTCATTAAGGATAAAAGTTATGACAAAGATACGTTAGTACTTAAG
GCAGCCGGAAACATTAATTCAGGTTATAAAAAGCCTAATCCAAAAGATTACAATTACTCA
CAGTTTTATTGGGGCGGTAAGTATAATGTTTCGGTTAGTTCAGAATCAAATGATGCTGTA
AATGTTGTTGACTATGCACCTAAAAATCAAAATGAAGAATTCCAAGTTCAACAAACATTA
GGTTATTCTTATGGCGGAGATATTAATATATCTAATGGCTTATCAGGTGGATTAAATGGA
TCAAAATCATTTTCAGAAACGATAAATTATAAACAAGAAAGTTACAGAACTACGATTGAT
AGAAAAACAAATCATAAATCAATTGGCTGGGGTGTTGAGGCGCACAAAATTATGAATAAT
GGTTGGGGACCATATGGTAGAGATAGTTATGACCCAACATATGGTAATGAACTGTTTTTA
GGCGGTAGACAAAGTAGTTCAAATGCTGGTCAAAATTTCTTGCCAACACATCAAATGCCT
TTATTGGCGCGTGGTAACTTTAACCCAGAATTTATAAGCGTACTTTCTCATAAACAAAAT
GATACAAAAAAATCTAAAATCAAAGTAACTTACCAAAGAGAAATGGATAGATATACTAAT
CAATGGAATCGACTACACTGGGTTGGTAATAACTACAAAAATCAAAATACAGTAACGTTT
ACATCTACTTATGAAGTTGACTGGCAAAACCATACTGTTAAATTAATCGGTACGGATTCT
AAAGAAACTAATCCTGGAGTATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL1880 [new locus tag: SACOL_RS09650 ]
- symbol: LukD
- description: leukotoxin LukD
- length: 327
- theoretical pI: 9.65379
- theoretical MW: 36888.7
- GRAVY: -0.815596
⊟Function[edit | edit source]
- TIGRFAM: Cellular processes Toxin production and resistance beta-channel forming cytolysin (TIGR01002; HMM-score: 441.5)
- TheSEED :
- Leukotoxin LukD
- PFAM: Leukocidin (CL0636) Leukocidin; Leukocidin/Hemolysin toxin family (PF07968; HMM-score: 296.9)and 1 moreno clan defined tRNA_synt_1c_R2; Glutaminyl-tRNA synthetase, non-specific RNA binding region part 2 (PF04557; HMM-score: 13.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Extracellular
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0.09
- Cellwall Score: 0.18
- Extracellular Score: 9.73
- Internal Helices: 0
- LocateP: Secretory(released) (with CS)
- Prediction by SwissProt Classification: Extracellular
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: -0.17
- Signal peptide possibility: 1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: NTVDAAQH
- SignalP: Signal peptide SP(Sec/SPI) length 26 aa
- SP(Sec/SPI): 0.995468
- TAT(Tat/SPI): 0.000385
- LIPO(Sec/SPII): 0.003085
- Cleavage Site: CS pos: 26-27. VDA-AQ. Pr: 0.9634
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITLYKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQFYWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNGSKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNELFLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMDRYTNQWNRLHWVGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Signal peptide containing [1] [2] [3]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: SaeR (activation) regulon
SaeR (TF) important in Virulence; RegPrecise transcription unit transferred from N315 data RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)