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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01954
  • pan locus tag?: SAUPAN004583000
  • symbol: SAOUHSC_01954
  • pan gene symbol?: lukD
  • synonym:
  • product: leukotoxin LukD

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01954
  • symbol: SAOUHSC_01954
  • product: leukotoxin LukD
  • replicon: chromosome
  • strand: -
  • coordinates: 1859010..1859993
  • length: 984
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAATGAAAAAATTAGTCAAATCATCAGTTGCTTCATCAATTGCACTGCTTTTGCTA
    TCGAATACAGTTGATGCAGCTCAACATATCACACCTGTAAGCGAGAAAAAAGTAGATGAC
    AAAATCACTTTATACAAAACAACAGCAACATCTGATAATGATAAATTGAATATTTCTCAA
    ATTTTAACGTTTAATTTCATTAAGGATAAAAGTTATGACAAAGATACGTTAGTACTTAAG
    GCAGCCGGAAACATTAATTCAGGTTATAAAAAGCCTAATCCAAAAGATTACAATTACTCA
    CAGTTTTATTGGGGCGGTAAGTATAATGTTTCGGTTAGTTCAGAATCAAATGATGCTGTA
    AATGTTGTTGACTATGCACCTAAAAATCAAAATGAAGAATTCCAAGTTCAACAAACATTA
    GGTTATTCTTATGGCGGAGATATTAATATATCTAATGGCTTATCAGGTGGATTAAATGGA
    TCAAAATCATTTTCAGAAACGATAAATTATAAACAAGAAAGTTACAGAACTACGATTGAT
    AGAAAAACAAATCATAAATCAATTGGCTGGGGTGTTGAGGCGCACAAAATTATGAATAAT
    GGTTGGGGACCATATGGTAGAGATAGTTATGACCCAACATATGGTAATGAACTGTTTTTA
    GGTGGTAGACAAAGTAGTTCAAATGCTGGTCAAAATTTCTTGCCAACACATCAAATGCCT
    TTATTGGCGCGTGGTAACTTTAACCCAGAATTTATAAGCGTACTTTCTCATAAACAAAAT
    GATACAAAAAAATCTAAAATCAAAGTAACTTACCAAAGAGAAATGGATAGATATACTAAT
    CAATGGAATCGACTACACTGGGTTGGAAATAACTACAAAAATCAAAATACAGTAACGTTT
    ACATCTACTTATGAAGTTGACTGGCAAAACCATACTGTTAAATTAATCGGTACGGATTCT
    AAAGAAACTAATCCTGGAGTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    984

⊟Protein[edit | edit source]

Protein Data Bank: 4Q7G

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01954
  • symbol: SAOUHSC_01954
  • description: leukotoxin LukD
  • length: 327
  • theoretical pI: 9.65379
  • theoretical MW: 36888.7
  • GRAVY: -0.815596

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance beta-channel forming cytolysin (TIGR01002; HMM-score: 441.5)
  • TheSEED  :
    • Leukotoxin LukD
    Virulence, Disease and Defense Toxins and superantigens Staphylococcus Two-component and Pore-forming Cytolysins  Leukotoxin LukD
  • PFAM:
    Leukocidin (CL0636) Leukocidin; Leukocidin/Hemolysin toxin family (PF07968; HMM-score: 296.9)
    and 1 more
    no clan defined tRNA_synt_1c_R2; Glutaminyl-tRNA synthetase, non-specific RNA binding region part 2 (PF04557; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: NTVDAAQH
  • SignalP: Signal peptide SP(Sec/SPI) length 26 aa
    • SP(Sec/SPI): 0.995468
    • TAT(Tat/SPI): 0.000385
    • LIPO(Sec/SPII): 0.003085
    • Cleavage Site: CS pos: 26-27. VDA-AQ. Pr: 0.9634
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITLYKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQFYWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNGSKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNELFLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMDRYTNQWNRLHWVGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR* (activation) regulon
    SaeR*(TF)important in Virulence; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Naoko Morinaga, Yuriko Kaihou, Masatoshi Noda
Purification, cloning and characterization of variant LukE-LukD with strong leukocidal activity of staphylococcal bi-component leukotoxin family.
Microbiol Immunol: 2003, 47(1);81-90
[PubMed:12636257] [WorldCat.org] [DOI] (P p)Jun Kaneko, Yoshiyuki Kamio
Bacterial two-component and hetero-heptameric pore-forming cytolytic toxins: structures, pore-forming mechanism, and organization of the genes.
Biosci Biotechnol Biochem: 2004, 68(5);981-1003
[PubMed:15170101] [WorldCat.org] [DOI] (P p)

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