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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1926 [new locus tag: SACOL_RS10070 ]
  • pan locus tag?: SAUPAN004850000
  • symbol: mutY
  • pan gene symbol?: mutY
  • synonym:
  • product: A/G-specific adenine glycosylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1926 [new locus tag: SACOL_RS10070 ]
  • symbol: mutY
  • product: A/G-specific adenine glycosylase
  • replicon: chromosome
  • strand: -
  • coordinates: 1990591..1991628
  • length: 1038
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTATCAGCAGTCTAGTTTTAAAGAAAATTTGATACACTGGTTTGATGAAAATCAAAGA
    GAAATGCCATGGCGACAAACGACCAATCCGTACTATATTTGGTTAAGTGAGGTCATGTTA
    CAACAGACACAAGTTAAAACAGTTATTGACTATTATCATCGTTTTGTTGAACGATTTCCA
    ACTGTGGAAGTTTTGAGTCAAGCTTCGGAAGATGAAGTGTTAAAGTATTGGGAAGGCTTA
    GGATATTATAGTAGAGCTCGAAATTTTCATACGGCGATTAAAGAAGTTCATGATAAATAT
    GAAGGCTTAGTTCCCAAAGATCCTGATCAATTTAAAGCGTTAAAAGGTGTTGGCCCATAC
    ACACAAGCTGCAGTAATGAGCATTGCATATAATGTACCACTAGCAACTGTAGACGGAAAT
    GTTTTTCGAGTATGGTCTAGATTGAATGATGACTATCGAGATATTAAATTACAATCGACG
    AGGAAATCGTACGAACAAGAATTATTACCGTATGTTACTACAGAAGCTGGAACGTTTAAT
    CAAGCTATGATGGAATTAGGGGCGTTAATTTGTACACCAAAAAATCCATTATGCTTATTT
    TGTCCAGTTCAAGAAAACTGTGAGGCATTTGATAAAGGTACATTTGAAAAGTTACCTGTT
    AAATCTAAAAATGTAAGTAAAAAAGTAATTGAGCAATCCGTCTTTTTAATTAGAAATAAT
    CAAGGTCAATATCTGTTGCAAAAGCGTAGCGAAAAATTATTGCATGGTATGTGGCAATTT
    CCTATGTTTGAAAGTGAGCATGCTAGACGTAAAATGACTGAAAAAATCGGTCATGACATT
    CAACCAGTAGAAACACCTATTTTTGAGTTGAAGCATCAATTTACTCATTTAACATGGAAG
    ATTAAAGTATACGCCGTTTCAGGAGCAATTAACATTGAAACATTACCAGATGACATGATT
    TGGTTTGATTTAAGTGATCGAGATCAATATACATTCCCTGTACCTATGTCAAAGATTTAT
    CAATTTATAAATGGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1038

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1926 [new locus tag: SACOL_RS10070 ]
  • symbol: MutY
  • description: A/G-specific adenine glycosylase
  • length: 345
  • theoretical pI: 6.79462
  • theoretical MW: 40623.2
  • GRAVY: -0.495652

⊟Function[edit | edit source]

  • reaction:
    EC 3.2.2.-?  ExPASy
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair A/G-specific adenine glycosylase (TIGR01084; EC 3.2.2.-; HMM-score: 328.3)
    and 1 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair endonuclease III (TIGR01083; EC 4.2.99.18; HMM-score: 74.1)
  • TheSEED  :
    • A/G-specific adenine glycosylase (EC 3.2.2.-)
    DNA Metabolism DNA repair DNA repair, bacterial  A/G-specific adenine glycosylase (EC 3.2.2.-)
  • PFAM:
    NUDIX (CL0261) NUDIX_4; NUDIX domain (PF14815; HMM-score: 78.6)
    and 5 more
    HHH (CL0198) HhH-GPD; HhH-GPD superfamily base excision DNA repair protein (PF00730; HMM-score: 55.7)
    HHH; Helix-hairpin-helix motif (PF00633; HMM-score: 28.1)
    no clan defined EndIII_4Fe-2S; Iron-sulfur binding domain of endonuclease III (PF10576; HMM-score: 16.9)
    NUDIX (CL0261) NUDIX; NUDIX domain (PF00293; HMM-score: 15.4)
    no clan defined NDUFB10; NADH-ubiquinone oxidoreductase subunit 10 (PF10249; HMM-score: 12.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002987
    • TAT(Tat/SPI): 0.000176
    • LIPO(Sec/SPII): 0.000808
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQVKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEGLVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQSTRKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKLPVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARRKMTEKIGHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPVPMSKIYQFING

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell: 35 [2]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  2. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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