PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1849 [new locus tag: SAUSA300_RS10100 ]
• pan locus tag^?: SAUPAN004850000
• symbol: mutY
• pan gene symbol^?: mutY
• synonym:
• product: A/G-specific adenine glycosylase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1849 [new locus tag: SAUSA300_RS10100 ]
• symbol: mutY
• product: A/G-specific adenine glycosylase
• replicon: chromosome
• strand: -
• coordinates: 2011237..2012274
• length: 1038
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914767 NCBI
• RefSeq: YP_494501 NCBI
• BioCyc: see SAUSA300_RS10100
• MicrobesOnline: 1293364 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1021
  
  ATGTATCAGCAGTCTAGTTTTAAAGAAAATTTGATACACTGGTTTGATGAAAATCAAAGA
  GAAATGCCATGGCGACAAACGACCAATCCGTACTATATTTGGTTAAGTGAGGTCATGTTA
  CAACAGACACAAGTTAAAACAGTTATTGACTATTATCATCGTTTTGTTGAACGATTTCCA
  ACTGTGGAAGTTTTGAGTCAAGCTTCGGAAGATGAAGTGTTAAAGTATTGGGAAGGCTTA
  GGATATTATAGTAGAGCTCGAAATTTTCATACGGCGATTAAAGAAGTTCATGATAAATAT
  GAAGGCTTAGTTCCCAAAGATCCTGATCAATTTAAAGCGTTAAAAGGTGTTGGCCCATAC
  ACACAAGCTGCAGTAATGAGCATTGCATATAATGTACCACTAGCAACTGTAGACGGAAAT
  GTTTTTCGAGTATGGTCTAGATTGAATGATGACTATCGAGATATTAAATTACAATCGACG
  AGGAAATCGTACGAACAAGAATTATTACCGTATGTTACTACAGAAGCTGGAACGTTTAAT
  CAAGCTATGATGGAATTAGGGGCGTTAATTTGTACACCAAAAAATCCATTATGCTTATTT
  TGTCCAGTTCAAGAAAACTGTGAGGCATTTGATAAAGGTACATTTGAAAAGTTACCTGTT
  AAATCTAAAAATGTAAGTAAAAAAGTAATTGAGCAATCCGTCTTTTTAATTAGAAATAAT
  CAAGGTCAATATCTGTTGCAAAAGCGTAGCGAAAAATTATTGCATGGTATGTGGCAATTT
  CCTATGTTTGAAAGTGAGCATGCTAGACGTAAAATGACTGAAAAAATCGGTCATGACATT
  CAACCAGTAGAAACACCTATTTTTGAGTTGAAGCATCAATTTACTCATTTAACATGGAAG
  ATTAAAGTATACGCCGTTTCAGGAGCAATTAACATTGAAACATTACCAGATGACATGATT
  TGGTTTGATTTAAGTGATCGAGATCAATATACATTCCCTGTACCTATGTCAAAGATTTAT
  CAATTTATAAATGGGTAA
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  1038
  

⊟Protein

⊟General

• locus tag: SAUSA300_1849 [new locus tag: SAUSA300_RS10100 ]
• symbol: MutY
• description: A/G-specific adenine glycosylase
• length: 345
• theoretical pI: 6.79462
• theoretical MW: 40623.2
• GRAVY: -0.495652

⊟Function

• reaction:
  EC 3.2.2.-?  ExPASy
• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair A/G-specific adenine glycosylase (TIGR01084; EC 3.2.2.-; HMM-score: 328.3)
  and 1 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair endonuclease III (TIGR01083; EC 4.2.99.18; HMM-score: 74.1)
• TheSEED  :
  • A/G-specific adenine glycosylase (EC 3.2.2.-)
  DNA Metabolism DNA repair DNA repair, bacterial  A/G-specific adenine glycosylase (EC 3.2.2.-)
• PFAM:
  NUDIX (CL0261) NUDIX_4; NUDIX domain (PF14815; HMM-score: 78.6)
  and 5 more
  HHH (CL0198) HhH-GPD; HhH-GPD superfamily base excision DNA repair protein (PF00730; HMM-score: 55.7)
  HHH; Helix-hairpin-helix motif (PF00633; HMM-score: 28.1)
  no clan defined EndIII_4Fe-2S; Iron-sulfur binding domain of endonuclease III (PF10576; HMM-score: 16.9)
  NUDIX (CL0261) NUDIX; NUDIX domain (PF00293; HMM-score: 15.4)
  no clan defined NDUFB10; NADH-ubiquinone oxidoreductase subunit 10 (PF10249; HMM-score: 12.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002987
  • TAT(Tat/SPI): 0.000176
  • LIPO(Sec/SPII): 0.000808
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161368 NCBI
• RefSeq: YP_494501 NCBI
• UniProt: A0A0H2XH44 UniProt

⊟Protein sequence

• MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQVKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEGLVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQSTRKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKLPVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARRKMTEKIGHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPVPMSKIYQFING

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications