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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1941 [new locus tag: SACOL_RS10150 ]
  • pan locus tag?: SAUPAN004898000
  • symbol: SACOL1941
  • pan gene symbol?: —
  • synonym:
  • product: ribonuclease BN

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1941 [new locus tag: SACOL_RS10150 ]
  • symbol: SACOL1941
  • product: ribonuclease BN
  • replicon: chromosome
  • strand: +
  • coordinates: 2003608..2004825
  • length: 1218
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTCGAAAAAGGATCACTCTTCTTCAAAATACCTTAATTCTGTTAAGGAAGCGCAAGAG
    GAGTCAAAAAAGAAAAATAAAAGTAATCCCAAAATTGATGTTGATCGTACATATATTGAA
    CCTCAACAATTCCAATCTAAGAAACCTAAAAAAGATGATCAGGTTTTCTTCTTATCAAGA
    TTAAATAAACCTGCAAAATATAAGAAAGACTCTAATTTCTTATCATATCTCATCTATCGC
    ATAGGAAAAGATGATGCCTCAGGACTAGCAGCACAAATGACTTACCATTTCGTACTTGCT
    ATGTTCCCTATGTTGCTTTTCCTATTAACATTATTACCATTTTTCAATATTAAGCAGAGT
    CAAATTACTAATATGTTAAGCAATGCACCCGCTGAAACATCTACTCTAATTAAGAGTGTA
    ATTGGTGATATAACTCAAAACTCCAGTGGTGGCTTATTATCTATCGGTTTGATTTTAGCA
    ATTTGGTCAGCTTCAAATGGAATGACTGCAATTATGAATTCTTTCAATGTTGCTTACGAT
    GTAGAAGATAGCCGTAATGGAATCGTATTAAAACTACTAAGTGTTGTCTTCACTGTAGTT
    ATGGGCGTTGTGTTTGTAGTTGCTCTAGCATTACCAACGCTTGGTTCTGTAATTAGTCAT
    TTCCTATTCGGTCCACTTGGATTTGACGAACAAGTGAAATGGATTTTTAACCTTATTAGA
    ATTGTGTTACCAATCATTATTATATTTATCATATTTATCGTGTTATATTCGGTTGCACCT
    AACGTTAAAACGAAGCTTAAGTCAGTATTACCAGGTGCAGTATTTACTTCAATTATTTGG
    TTAGCTGGTTCATTTGGTTTTGGTTGGTATATTTCAAATTTTGGTAACTATTCTAAAACA
    TATGGCAGTATCGCGGGTATCATCATTTTGTTACTATGGTTATATATCACAAGTTTTATT
    ATAATTGTCGGTGCTGAAATCAATGCAATCATTCATCAGCGTAGTGTAATTAAAGGTAAA
    ACACCTGAAGAAGCAGCATTAGAACATGATGACAATAATAAAAATCATTATAATGAGAAC
    ACTAGATACGAATATGATGACGATAGTAATGCAACACATCAACGTACGTATCATGTTGAT
    GAACATCCTAGTGATACCTATCCAGAAGATGATAAAAATATAAAAGACAAAATCGTTGAT
    AAGCTTAAAAAAGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1218

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1941 [new locus tag: SACOL_RS10150 ]
  • symbol: SACOL1941
  • description: ribonuclease BN
  • length: 405
  • theoretical pI: 9.26901
  • theoretical MW: 45590.4
  • GRAVY: 0.0162963

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function General YihY family inner membrane protein (TIGR00765; HMM-score: 165.5)
    and 1 more
    Genetic information processing Transcription Degradation of RNA inner membrane protein YhjD (TIGR00766; HMM-score: 33.6)
  • TheSEED  :
    • Ribonuclease BN (EC 3.1.-.-)
    RNA Metabolism RNA processing and modification tRNA processing  Ribonuclease BN (EC 3.1.-.-)
  • PFAM:
    no clan defined Virul_fac_BrkB; Virulence factor BrkB (PF03631; HMM-score: 234.3)
    and 2 more
    DUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 11.4)
    Oxa1 (CL0376) 60KD_IMP; 60Kd inner membrane protein (PF02096; HMM-score: 10.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.015037
    • TAT(Tat/SPI): 0.002293
    • LIPO(Sec/SPII): 0.002907
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQFQSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMFPMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILAIWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVISHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIIFIVLYSVAPNVKTKLKSVLPGAVFTSIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQRSVIKGKTPEEAALEHDDNNKNHYNENTRYEYDDDSNATHQRTYHVDEHPSDTYPEDDKNIKDKIVDKLKKD

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB* (activation) regulon
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [3] [4]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  4. ↑ Jan Pané-Farré, Beate Jonas, Konrad Förstner, Susanne Engelmann, Michael Hecker
    The sigmaB regulon in Staphylococcus aureus and its regulation.
    Int J Med Microbiol: 2006, 296(4-5);237-58
    [PubMed:16644280] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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