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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2006 [new locus tag: SACOL_RS10480 ]
  • pan locus tag?: SAUPAN005192000
  • symbol: SACOL2006
  • pan gene symbol?: lukH
  • synonym: lukA
  • product: Aerolysin/leukocidin family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2006 [new locus tag: SACOL_RS10480 ]
  • symbol: SACOL2006
  • product: Aerolysin/leukocidin family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2065994..2067049
  • length: 1056
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAAAATAAAAAACGTGTTTTAATAGCGTCATCATTATCATGTGCAATTTTATTGTTA
    TCAGCAGCAACGACTCAAGCAAATTCAGCTCATAAAGACTCTCAAGACCAAAATAAGAAA
    GAACATGTTGATAAGTCTCAACAAAAAGACAAACGTAATGTTACTAATAAAGATAAAAAT
    TCAACAGCACCGGATGATATTGGGAAAAACGGTAAAATCACAAAACGAACTGAAACAGTA
    TATGATGAGAAAACAAATATACTCCAAAATTTACAATTCGACTTTATCGATGATCCAACT
    TATGACAAGAATGTATTACTTGTTAAAAAACAAGGCTCAATTCATTCAAATTTAAAGTTT
    GAATCTCATAAAGAAGAAAAAAATTCAAATTGGTTAAAGTATCCAAGTGAGTACCATGTA
    GATTTTCAAGTAAAAAGAAATCGTAAAACTGAAATATTAGACCAATTGCCGAAAAATAAA
    ATTTCAACTGCAAAAGTAGACAGTACATTTTCATATAGCTCAGGTGGTAAATTCGATTCA
    ACAAAAGGTATTGGACGAACTTCATCAAATAGCTACTCCAAAACGATTAGTTATAATCAG
    CAAAATTATGACACAATTGCCAGCGGTAAAAATAATAACTGGCATGTACACTGGTCAGTT
    ATTGCGAATGACTTGAAGTATGGTGGAGAAGTGAAAAATAGAAATGATGAATTATTATTC
    TATAGAAATACGAGAATTGCTACTGTAGAAAACCCTGAACTAAGCTTTGCTTCAAAATAT
    AGATACCCAGCATTAGTAAGAAGTGGCTTTAATCCAGAATTTTTAACTTATTTATCTAAT
    GAAAAGTCAAATGAGAAAACGCAATTTGAAGTAACATACACACGAAATCAAGATATTTTG
    AAAAACAGACCTGGAATACATTATGCACCTCCAATTTTAGAAAAAAATAAAGATGGTCAA
    AGATTAATTGTCACTTATGAAGTTGATTGGAAAAATAAAACAGTTAAAGTCGTTGATAAA
    TATTCTGATGACAATAAACCTTATAAAGAAGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1056

⊟Protein[edit | edit source]

Protein Data Bank: 5K59

⊟General[edit | edit source]

  • locus tag: SACOL2006 [new locus tag: SACOL_RS10480 ]
  • symbol: SACOL2006
  • description: Aerolysin/leukocidin family protein
  • length: 351
  • theoretical pI: 9.95044
  • theoretical MW: 40433.8
  • GRAVY: -1.05242

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance beta-channel forming cytolysin (TIGR01002; HMM-score: 190)
  • TheSEED  :
    • Leukocidin LukS-H
  • PFAM:
    Leukocidin (CL0636) Leukocidin; Leukocidin/Hemolysin toxin family (PF07968; HMM-score: 250.6)
    and 2 more
    no clan defined GRP; Glycine rich protein family (PF07172; HMM-score: 14.3)
    Omega_toxin (CL0083) Conotoxin; Conotoxin (PF02950; HMM-score: 11.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: TTQANSAH
  • SignalP: Signal peptide SP(Sec/SPI) length 29 aa
    • SP(Sec/SPI): 0.774577
    • TAT(Tat/SPI): 0.00088
    • LIPO(Sec/SPII): 0.223907
    • Cleavage Site: CS pos: 29-30. ANS-AH. Pr: 0.3455
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVDKSQQKDKRNVTNKDKNSTAPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYDKNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNRKTEILDQLPKNKISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHWSVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLTYLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKDGQRLIVTYEVDWKNKTVKVVDKYSDDNKPYKEG

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Lipoprotein [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Stephan Fuchs, Jan Pané-Farré, Christian Kohler, Michael Hecker, Susanne Engelmann
Anaerobic gene expression in Staphylococcus aureus.
J Bacteriol: 2007, 189(11);4275-89
[PubMed:17384184] [WorldCat.org] [DOI] (P p)

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