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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2046 [new locus tag: SACOL_RS10685 ]
  • pan locus tag?: SAUPAN005307000
  • symbol: leuA
  • pan gene symbol?: leuA
  • synonym:
  • product: 2-isopropylmalate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2046 [new locus tag: SACOL_RS10685 ]
  • symbol: leuA
  • product: 2-isopropylmalate synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 2105979..2107508
  • length: 1530
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAGTAGTCATATTCAAATTTTTGATACGACACTAAGAGACGGTGAACAAACACCAGGA
    GTGAATTTTACTTTTGATGAACGCTTGCGTATTGCATTGCAATTAGAAAAATGGGGTGTA
    GATGTTATTGAAGCTGGATTTCCTGCTTCAAGTACAGGTAGCTTTAAATCTGTTCAAGCA
    ATTGCACAAACATTAACAACAACGGCTGTATGTGGTTTAGCTAGATGTAAAAAATCTGAC
    ATCGATGCTGTATATGAAGCAACAAAAGATGCAGCGAAGCCGGTCGTGCATGTTTTTATA
    GCAACATCACCTATTCATCTTGAACATAAACTTAAAATGTCTCAAGAAGACGTTTTAGCA
    TCTATTAAAGAACATGTCACATACGCGAAACAATTATTTGACGTTGTTCAATTTTCACCT
    GAAGATGCAACGCGTACTGAATTACCATTCTTAGTGAAATGTGTACAAACTGCCGTTGAC
    GCTGGAGCTACAGTTATTAATATTCCTGATACAGTCGGCTACAGTTACCATGATGAATAT
    GCACATATTTTCAAAACCTTAACAGAATCTGTAACATCTTCAAATGAAATTATTTATAGT
    GCTCATTGCCATGACGATTTAGGAATGGCTGTTTCAAATAGTTTAGCTGCAATTGAAGGC
    GGTGCGAGACGAATTGAAGGCACTGTAAATGGTATTGGTGAACGAGCAGGTAATGCAGCA
    CTTGAAGAAGTCGCGCTTGCACTATACGTTCGAAATGATCATTATGGTGCTCAAACTGCT
    CTTAATCTCGAAGAAACTAAAAAAACATCGGATTTAATTTCAAGATATGCAGGTATTCGA
    GTGCCTAGAAATAAAGCAATTGTTGGCCAAAATGCATTTAGTCATGAATCAGGTATTCAC
    CAAGATGGCGTATTAAAACATCGTGAAACATATGAAATTATGACACCTCAACTTGTTGGT
    GTAAGCACGACTGAACTTCCATTAGGAAAATTATCTGGTAAACACGCCTTCTCAGAGAAG
    TTAAAAGCATTAGGTTATGACATTGATAAAGAAGCGCAAATAGATTTATTTAAACAATTC
    AAGGCCATTGCGGACAAAAAGAAATCTGTTTCAGATAGAGATATTCATGCGATTATTCAA
    GGTTCTGAGCATGAGCATCAAGCACTTTATAAATTGGAAACACTGCAACTACAATATGTC
    TCTAGCGGCCTTCAAAGTGCTGTTGTTGTTGTTAAAGATAAAGAGGGTCATATTTACCAG
    GATTCAAGTATTGGTACTGGTTCAATCGTAGCAATTTACAATGCAGTTGATCGTATTTTC
    CAGAAAGAAACAGAATTAATTGATTATCGTATTAATTCTGTCACTGAAGGTACTGATGCC
    CAAGCAGAAGTACATGTAAATTTATTGATTGAAGGTAAGACTGTCAATGGCTTTGGTATT
    GATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCA
    GCTGAAAATGTTGAGAAGGTAGGTAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1530

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2046 [new locus tag: SACOL_RS10685 ]
  • symbol: LeuA
  • description: 2-isopropylmalate synthase
  • length: 509
  • theoretical pI: 5.75975
  • theoretical MW: 55674.3
  • GRAVY: -0.198821

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.3.13?  ExPASy
    2-isopropylmalate synthase Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Pyruvate family 2-isopropylmalate synthase (TIGR00973; EC 2.3.3.13; HMM-score: 662.6)
    and 9 more
    isopropylmalate/citramalate/homocitrate synthases (TIGR02090; HMM-score: 405)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other homocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 315.2)
    Metabolism Central intermediary metabolism Nitrogen fixation homocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 315.2)
    homocitrate synthase (TIGR02146; EC 2.3.3.14; HMM-score: 282.4)
    Unknown function General citramalate synthase (TIGR00977; EC 2.3.1.182; HMM-score: 207.9)
    Metabolism Amino acid biosynthesis Pyruvate family 2-isopropylmalate synthase (TIGR00970; EC 2.3.3.13; HMM-score: 128)
    Metabolism Energy metabolism Other 4-hydroxy-2-oxovalerate aldolase (TIGR03217; EC 4.1.3.39; HMM-score: 75.6)
    Metabolism Transport and binding proteins Cations and iron carrying compounds oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 15.1)
    Metabolism Energy metabolism Other oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 15.1)
  • TheSEED  :
    • 2-isopropylmalate synthase (EC 2.3.3.13)
    Amino Acids and Derivatives Branched-chain amino acids Branched-Chain Amino Acid Biosynthesis  2-isopropylmalate synthase (EC 2.3.3.13)
    and 1 more
    Amino Acids and Derivatives Branched-chain amino acids Leucine Biosynthesis  2-isopropylmalate synthase (EC 2.3.3.13)
  • PFAM:
    TIM_barrel (CL0036) HMGL-like; HMGL-like (PF00682; HMM-score: 311.9)
    and 1 more
    no clan defined LeuA_dimer; LeuA allosteric (dimerisation) domain (PF08502; HMM-score: 122.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005044
    • TAT(Tat/SPI): 0.002612
    • LIPO(Sec/SPII): 0.000366
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIEAGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIATSPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVDAGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAIEGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRYAGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGKHAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKLETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYRINSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVGN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell: 2681 [5]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. ↑ Charlotte D Majerczyk, Paul M Dunman, Thanh T Luong, Chia Y Lee, Marat R Sadykov, Greg A Somerville, Kip Bodi, Abraham L Sonenshein
    Direct targets of CodY in Staphylococcus aureus.
    J Bacteriol: 2010, 192(11);2861-77
    [PubMed:20363936] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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