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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2103 [new locus tag: SACOL_RS11005 ]
  • pan locus tag?: SAUPAN005402000
  • symbol: SACOL2103
  • pan gene symbol?: mnaA
  • synonym:
  • product: UDP-GlcNAc 2-epimerase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2103 [new locus tag: SACOL_RS11005 ]
  • symbol: SACOL2103
  • product: UDP-GlcNAc 2-epimerase
  • replicon: chromosome
  • strand: -
  • coordinates: 2163782..2164909
  • length: 1128
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAAAAAGATTATGACCATATTTGGAACAAGACCCGAAGCCATAAAGATGGCTCCTTTA
    GTTAAAGCTTTGGAACAAGAAAAAATGCTCGAGCCAATTGTTGTTGTTACAGCACAGCAT
    AGAGAAATGCTTGATTCAGTATTAAGTACCTTTGAAATCAAACCAAAATACGATTTGAAT
    ATTATGAAATCAGGACAGACACTTTCTGAGATAACTTCTAAATCAATCACTCAATTAGAA
    CAAGTGATTCAATTAGAAAAACCAGACATGGTACTTGTACATGGTGATACGATGACGACA
    TTTGCAGGTGGTTTAGCGGCATTTTATAACCAAGTGCCGATTGGACACGTTGAAGCAGGG
    CTTAGAAGTTATGATAAATATTCACCTTTTCCAGAAGAGGTGAATAGACAATTAGTTGGT
    GTATTAGCTGACTTACATTTTGCACCTACTAAAAATGCTGCATCGCATTTGTTAAGTGAA
    GGGAAATATTCAGAAAGTGTTGTTGTCACTGGTAATACTGCGATTGATGCTATGAAATAT
    ACAGTTGATGACAATTATAAATCTAACATCATGGATAAATACCATGACAAAAAATTCATT
    CTTATGACAGCACACCGACGAGAAAATATCGGGAAACCCATGGAAAATATATTTAAAGCG
    GTTAGACGCTTAATAGATGAGTATACTGATTTAGCACTGGTATATCCAATGCATAAAAAC
    CCGAAAGTTCGAGAGGTGGCTCAAAAGATTTTAGGTAGTCATGACAGAATTGAATTGATT
    GAACCTTTGGACGTAGTAGATTTCCATAATTTTGCTAAAAAATCTTATTTTATTTTGACA
    GATTCAGGTGGAATTCAGGAAGAAGCGCCATCATTTAATAAGCCAGTTTTAGTTTTGCGA
    AGTGTTACAGAACGTCCTGAAGGTGTTGAAGCTGGCACATTAAAAGTCATTGGAACAAAT
    AAGCAAAATGTGTATCAAGCTGCAAAAGAATTAATCGATGATGAGAGATTATATCATCAA
    ATGTCTGAAGCATCTAATCCTTACGGTGATGGATTTGCTTCAGAAAGAATAGTTAATCAT
    ATTAAGTATTATTTGAATTTAATCACTGAAAAACCAAGCGATTTCTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1128

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2103 [new locus tag: SACOL_RS11005 ]
  • symbol: SACOL2103
  • description: UDP-GlcNAc 2-epimerase
  • length: 375
  • theoretical pI: 6.52172
  • theoretical MW: 42412.4
  • GRAVY: -0.319733

⊟Function[edit | edit source]

  • reaction:
    EC 5.1.3.14?  ExPASy
    UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing) UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-mannosamine
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-N-acetylglucosamine 2-epimerase (TIGR00236; EC 5.1.3.14; HMM-score: 529.4)
    and 5 more
    UDP-N-acetyl-D-glucosamine 2-epimerase, UDP-hydrolysing (TIGR03568; EC 3.1.4.-,5.1.3.-; HMM-score: 122.5)
    putative cofactor-binding repeat (TIGR03807; HMM-score: 15.7)
    Metabolism Energy metabolism Amino acids and amines aspartate 4-decarboxylase (TIGR03801; EC 4.1.1.12; HMM-score: 15)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides lipid-A-disaccharide synthase (TIGR00215; EC 2.4.1.182; HMM-score: 13.9)
    conserved hypothetical protein (TIGR03492; HMM-score: 13.7)
  • TheSEED  :
    • UDP-N-acetylglucosamine 2-epimerase (EC 5.1.3.14)
    Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  UDP-N-acetylglucosamine 2-epimerase (EC 5.1.3.14)
  • PFAM:
    GT-B (CL0113) Epimerase_2; UDP-N-acetylglucosamine 2-epimerase (PF02350; HMM-score: 410.3)
    and 2 more
    Glyco_trans_4_2; Glycosyl transferase 4-like (PF13477; HMM-score: 12.6)
    GT-A (CL0110) NTP_transferase; Nucleotidyl transferase (PF00483; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00832
    • TAT(Tat/SPI): 0.000193
    • LIPO(Sec/SPII): 0.000496
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREMLDSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFAGGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSEGKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIFKAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSYFILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDDERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 90 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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