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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2159 [new locus tag: SACOL_RS11350 ]
  • pan locus tag?: SAUPAN005557000
  • symbol: SACOL2159
  • pan gene symbol?: sdrM
  • synonym:
  • product: drug transporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2159 [new locus tag: SACOL_RS11350 ]
  • symbol: SACOL2159
  • product: drug transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2237711..2239054
  • length: 1344
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCGATTAAAGTCAATCATAACTGTTATAGCTTTAATATTAATTATGTTTATGTCAGCG
    ATTGAATCATCTATTATTTCGCTTGCATTACCTACAATTAAACAAGATTTAAATGCAGGA
    AATTTAATTTCATTAATTTTCACAGCGTATTTTATCGCATTAGTAATTGCCAATCCTATA
    GTGGGCGAATTACTTTCGAGATTTAAAATTATTTATGTGGCAATTGCAGGATTACTTTTA
    TTTAGTATAGGTAGTTTTATGTGTGGTTTAAGTACTAATTTTACTATGCTAATTATATCA
    CGCGTGATACAAGGTTTTGGATCAGGTGTATTGATGTCATTATCGCAAATTGTTCCAAAG
    TTAGCATTTGAAATTCCGTTACGATATAAAATTATGGGCATAGTTGGCAGTGTTTGGGGT
    ATTTCGAGTATCATTGGGCCACTTCTAGGTGGTGGTATATTAGAATTTGCAACATGGCAT
    TGGTTATTCTACATAAATATACCGATTGCGATCATTGCCATTATATTAGTTATATGGACA
    TTTCATTTTCCAGAGGAAGAAACAGTTGCTAAATCAAAATTTGATACTAAAGGGCTCACG
    CTATTTTATGTATTTATTGGATTGATTATGTTTGCTTTATTAAACCAACAGCTGTTACTT
    TTAAATTTCTTGAGTTTTATCTTAGCTATCGTTGTGGCAATGTGTTTATTTAAAGTAGAA
    AAACATGTTTCTTCACCATTTTTACCTGTGGTTGAATTTAATCGTTCGATTACTTTAGTT
    TTTATAACTGACCTTCTAACAGCTATTTGTTTAATGGGATTCAATTTATATATTCCAGTC
    TACCTTCAAGAACAACTAGGATTATCTCCATTGCAAAGTGGATTGGTTATTTTTCCTTTA
    TCTGTAGCTTGGATTACATTGAATTTTAATTTACATCGAATTGAAGCAAAACTATCAAGG
    AAAGTTATTTACTTACTATCATTTACATTGCTACTAGTAAGTAGTATTATCATTTCATTT
    GGTATTAAATTGCCGGTACTTATAGCATTTGTGTTAATTTTGGCAGGATTAAGTTTTGGA
    TATATTTATACGAAAGATAGTGTGATTGTCCAAGAGGAAACTAGCCCATTACAAATGAAG
    AAAATGATGTCATTTTATGGATTAACTAAAAATCTTGGGGCATCAATAGGTTCAACAATA
    ATGGGATATCTTTATGCGATACAATCAGGAATCTTTGGTCCAAACTTACACAATGTGTTA
    AGTGCTGTTGCTGTAATTAGCATTGGGCTTATTGTTTTATGGGTCGTATTTTTTAAAGAA
    CAGTCATCTCAATCAAAAGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1344

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2159 [new locus tag: SACOL_RS11350 ]
  • symbol: SACOL2159
  • description: drug transporter
  • length: 447
  • theoretical pI: 9.4822
  • theoretical MW: 49409.4
  • GRAVY: 1.08523

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 160.6)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 160.6)
    and 8 more
    multidrug resistance protein (TIGR00880; HMM-score: 72.1)
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 58.9)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 52.3)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 38.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 31.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 29.6)
    Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 24.6)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 8.2)
  • TheSEED  :
    • Uncharacterized MFS-type transporter
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 183.3)
    and 2 more
    Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 28)
    TRI12; Fungal trichothecene efflux pump (TRI12) (PF06609; HMM-score: 23.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 29 aa
    • SP(Sec/SPI): 0.481061
    • TAT(Tat/SPI): 0.000733
    • LIPO(Sec/SPII): 0.150295
    • Cleavage Site: CS pos: 29-30. SLA-LP. Pr: 0.2959
  • predicted transmembrane helices (TMHMM): 14

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRLKSIITVIALILIMFMSAIESSIISLALPTIKQDLNAGNLISLIFTAYFIALVIANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRVIQGFGSGVLMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWHWLFYINIPIAIIAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQLLLLNFLSFILAIVVAMCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNLYIPVYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVSSIIISFGIKLPVLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNLGASIGSTIMGYLYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Youichi Yamada, Koh-ichiro Hideka, Sumiko Shiota, Teruo Kuroda, Tomofusa Tsuchiya
Gene cloning and characterization of SdrM, a chromosomally-encoded multidrug efflux pump, from Staphylococcus aureus.
Biol Pharm Bull: 2006, 29(3);554-6
[PubMed:16508166] [WorldCat.org] [DOI] (P p)

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