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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02420
  • pan locus tag?: SAUPAN005557000
  • symbol: SAOUHSC_02420
  • pan gene symbol?: sdrM
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02420
  • symbol: SAOUHSC_02420
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2248363..2249706
  • length: 1344
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCGATTAAAGTCAATCATAACTGTTATAGCTTTAATATTAATTATGTTTATGTCAGCG
    ATTGAATCATCTATTATTTCGCTTGCATTACCTACAATTAAACAAGATTTAAATGCAGGA
    AATTTAATTTCATTAATTTTCACAGCGTATTTTATCGCATTAGTAATTGCCAATCCTATA
    GTGGGCGAATTACTTTCGAGATTTAAAATTATTTATGTGGCAATTGCAGGATTACTTTTA
    TTTAGTATAGGTAGTTTTATGTGTGGTTTAAGTACTAATTTTACTATGCTAATTATATCA
    CGCGTGATACAAGGTTTTGGATCAGGTGTATTGATGTCATTATCGCAAATTGTTCCAAAG
    TTAGCATTTGAAATTCCGTTACGATATAAAATTATGGGCATAGTTGGCAGTGTTTGGGGT
    ATTTCGAGTATCATTGGGCCACTTCTAGGTGGTGGTATATTAGAATTTGCAACATGGCAT
    TGGTTATTCTACATAAATATACCGATTGCGATCATTGCCATTATATTAGTTATATGGACA
    TTTCATTTTCCAGAGGAAGAAACAGTTGCTAAATCAAAATTTGATACTAAAGGGCTCACG
    CTATTTTATGTATTTATTGGATTGATTATGTTTGCTTTATTAAACCAACAGCTGTTACTT
    TTAAATTTCTTGAGTTTTATCTTAGCTATCGTTGTGGCAATGTGTTTATTTAAAGTAGAA
    AAACATGTTTCTTCACCATTTTTACCTGTGGTTGAATTTAATCGTTCGATTACTTTAGTT
    TTTATAACTGACCTTCTAACAGCTATTTGTTTAATGGGATTCAATTTATATATTCCAGTC
    TACCTTCAAGAACAACTAGGATTATCTCCATTGCAAAGTGGATTGGTTATTTTTCCTTTA
    TCTGTAGCTTGGATTACATTGAATTTTAATTTACATCGAATTGAAGCAAAACTATCAAGG
    AAAGTTATTTACTTACTATCATTTACATTGCTACTAGTAAGTAGTATTATCATTTCATTT
    GGTATTAAATTGCCGGTACTTATAGCATTTGTGTTAATTTTGGCAGGATTAAGTTTTGGA
    TATATTTATACGAAAGATAGTGTGATTGTCCAAGAGGAAACTAGCCCATTACAAATGAAG
    AAAATGATGTCATTTTATGGATTAACTAAAAATCTTGGGGCATCAATAGGTTCAACAATA
    ATGGGATATCTTTATGCGATACAATCAGGAATCTTTGGTCCAAACTTACACAATGTGTTA
    AGTGCTGTTGCTGTAATTAGCATTGGGCTTATTGTTTTATGGGTCGTATTTTTTAAAGAA
    CAGTCATCTCAATCAAAAGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1344

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02420
  • symbol: SAOUHSC_02420
  • description: hypothetical protein
  • length: 447
  • theoretical pI: 9.4822
  • theoretical MW: 49409.4
  • GRAVY: 1.08523

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 160.6)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 160.6)
    and 8 more
    multidrug resistance protein (TIGR00880; HMM-score: 72.1)
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 58.9)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 52.3)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 38.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 31.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 29.6)
    Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 24.6)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 8.2)
  • TheSEED  :
    • Uncharacterized MFS-type transporter
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 183.3)
    and 2 more
    Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 28)
    TRI12; Fungal trichothecene efflux pump (TRI12) (PF06609; HMM-score: 23.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 29 aa
    • SP(Sec/SPI): 0.481061
    • TAT(Tat/SPI): 0.000733
    • LIPO(Sec/SPII): 0.150295
    • Cleavage Site: CS pos: 29-30. SLA-LP. Pr: 0.2959
  • predicted transmembrane helices (TMHMM): 14

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRLKSIITVIALILIMFMSAIESSIISLALPTIKQDLNAGNLISLIFTAYFIALVIANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRVIQGFGSGVLMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWHWLFYINIPIAIIAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQLLLLNFLSFILAIVVAMCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNLYIPVYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVSSIIISFGIKLPVLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNLGASIGSTIMGYLYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Youichi Yamada, Koh-ichiro Hideka, Sumiko Shiota, Teruo Kuroda, Tomofusa Tsuchiya
Gene cloning and characterization of SdrM, a chromosomally-encoded multidrug efflux pump, from Staphylococcus aureus.
Biol Pharm Bull: 2006, 29(3);554-6
[PubMed:16508166] [WorldCat.org] [DOI] (P p)

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