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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2188 [new locus tag: SACOL_RS11505 ]
  • pan locus tag?: SAUPAN005594000
  • symbol: lacR
  • pan gene symbol?: lacR
  • synonym:
  • product: lactose phosphotransferase system repressor

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2188 [new locus tag: SACOL_RS11505 ]
  • symbol: lacR
  • product: lactose phosphotransferase system repressor
  • replicon: chromosome
  • strand: -
  • coordinates: 2269241..2269996
  • length: 756
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    TTGAATAAACATGAACGTTTGGACGAAATTGCTAAACTAGTGAATAAAAAGGGCACGATA
    AGAACGAATGAAATCGTCGAAGGTTTAAATGTGTCTGATATGACAGTTCGAAGAGATTTA
    ATTGAATTGGAAAATAAAGGGATTTTAACGAAGATTCATGGTGGTGCACGCAGTAATTCA
    ACATTTCAGTATAAAGAAATATCGCATAAAGAAAAACATACCCGTCAAATTGCAGAAAAA
    CGATTTATAGCTAAAAAAGCTGCATCATTAATTGAAGATGGGGATACTTTGTTTTTCGGA
    CCAGGAACAACAGTGGAACTATTAGCAGAAGAAGTCAATCATCATACGCTCACAATTATT
    ACGAATTGTTTGCCGGTGTATAAAATTTTGTTAGAAAAACAAACAGCACATTTTCGTGTC
    TATTTAATTGGTGGTGAAATGCGCCATATTACAGAAGCATTTGTAGGTGAAATGGCGAAC
    GCCATGTTGGAAAAACTAAGATTTAGCAAGATGTTCTTTAGTAGTAACGCAGTAAATAAA
    GGTGCTGTGATGACATCTACATTAGATGAAGCCTATACGCAACAACTGGCACTAAGTAAT
    TCAATTGAAAAATACTTGTTAATCGATCATACGAAAGTTGGCAAAGAAGATTTTACATCA
    TTTTGTCAGTTAAACGAATTGACTGCAGTGGTCATGGACTATGAAGATGAAGAAAAAGTA
    GAAACGATTAAAACATATATTGAAGTAGTCGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    756

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2188 [new locus tag: SACOL_RS11505 ]
  • symbol: LacR
  • description: lactose phosphotransferase system repressor
  • length: 251
  • theoretical pI: 6.24703
  • theoretical MW: 28548.6
  • GRAVY: -0.293626

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 23.8)
    and 4 more
    Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 15.8)
    Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 15.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other iron-sulfur cluster biosynthesis transcriptional regulator SufR (TIGR02702; HMM-score: 14.7)
    Signal transduction Regulatory functions DNA interactions iron-sulfur cluster biosynthesis transcriptional regulator SufR (TIGR02702; HMM-score: 14.7)
  • TheSEED  :
    • Lactose phosphotransferase system repressor
    Carbohydrates Di- and oligosaccharides Lactose and Galactose Uptake and Utilization  Lactose phosphotransferase system repressor
  • PFAM:
    ISOCOT_Fold (CL0246) DeoRC; DeoR C terminal sensor domain (PF00455; HMM-score: 162.3)
    and 10 more
    HTH (CL0123) HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 81)
    HTH_11; HTH domain (PF08279; HMM-score: 27.5)
    GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 21.3)
    FaeA; FaeA-like protein (PF04703; HMM-score: 17.6)
    TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 16.3)
    Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 14.8)
    Penicillinase_R; Penicillinase repressor (PF03965; HMM-score: 14)
    ISOCOT_Fold (CL0246) CoA_trans; Coenzyme A transferase (PF01144; HMM-score: 12.6)
    HTH (CL0123) Rrf2; Transcriptional regulator (PF02082; HMM-score: 12.6)
    PaaX; PaaX-like protein (PF07848; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • genes regulated by LacR, TF important in Lactose utilization: in N315

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.024817
    • TAT(Tat/SPI): 0.00181
    • LIPO(Sec/SPII): 0.000503
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKHERLDEIAKLVNKKGTIRTNEIVEGLNVSDMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASLIEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMRHITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYLLIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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