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NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_02456
- pan locus tag?: SAUPAN005594000
- symbol: SAOUHSC_02456
- pan gene symbol?: lacR
- synonym:
- product: lactose phosphotransferase system repressor
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_02456
- symbol: SAOUHSC_02456
- product: lactose phosphotransferase system repressor
- replicon: chromosome
- strand: -
- coordinates: 2279892..2280683
- length: 792
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3919019 NCBI
- RefSeq: YP_500923 NCBI
- BioCyc: G1I0R-2321 BioCyc
- MicrobesOnline: 1290894 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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781ATGACCCATCGCCTAAATAAAGGAGGCAATTTTACATTGAATAAACATGAACGTTTGGAC
GAAATTGCTAAACTAGTGAATAAAAAGGGCACGATAAGAACGAATGAAATCGTCGAAGGT
TTAAATGTGTCTGATATGACAGTTCGAAGAGATTTAATTGAATTGGAAAATAAAGGGATT
TTAACGAAGATTCATGGTGGTGCACGCAGTAATTCAACATTTCAGTATAAAGAAATATCG
CATAAAGAAAAACATACCCGTCAAATTGCAGAAAAACGATTTATAGCTAAAAAAGCTGCA
TCATTAATTGAAGATGGGGATACTTTGTTTTTCGGACCAGGAACAACAGTGGAACTATTA
GCAGAAGAAGTCAATCATCATACGCTCACAATTATTACGAATTGTTTGCCGGTGTATAAA
ATTTTGTTAGAAAAACAAACAGCACATTTTCGTGTCTATTTAATTGGTGGTGAAATGCGC
CATATTACAGAAGCATTTGTAGGTGAAATGGCGAACGCCATGTTGGAAAAACTAAGATTT
AGCAAGATGTTCTTTAGTAGTAACGCAGTAAATAAAGGTGCTGTGATGACATCTACATTA
GATGAAGCCTATACGCAACAACTGGCACTAAGTAATTCAATTGAAAAATACTTGTTAATC
GATCATACGAAAGTTGGCAAAGAAGATTTTACATCATTTTGTCAGTTAAACGAATTGACT
GCAGTGGTCATGGACTATGAAGATGAAGAAAAAGTAGAAACGATTAAAACATATATTGAA
GTAGTCGACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_02456
- symbol: SAOUHSC_02456
- description: lactose phosphotransferase system repressor
- length: 263
- theoretical pI: 6.68378
- theoretical MW: 29888.1
- GRAVY: -0.319772
⊟Function[edit | edit source]
- TIGRFAM: Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 24.6)and 5 moreEnergy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 15.7)Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 15.7)Biosynthesis of cofactors, prosthetic groups, and carriers Other iron-sulfur cluster biosynthesis transcriptional regulator SufR (TIGR02702; HMM-score: 14.6)Regulatory functions DNA interactions iron-sulfur cluster biosynthesis transcriptional regulator SufR (TIGR02702; HMM-score: 14.6)Regulatory functions DNA interactions staphylococcal accessory regulator family (TIGR01889; HMM-score: 12.1)
- TheSEED :
- Lactose phosphotransferase system repressor
- PFAM: ISOCOT_Fold (CL0246) DeoRC; DeoR C terminal sensor domain (PF00455; HMM-score: 162.1)and 10 moreHTH (CL0123) HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 81)HTH_11; HTH domain (PF08279; HMM-score: 27.4)GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 21.2)FaeA; FaeA-like protein (PF04703; HMM-score: 17.5)TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 16.1)Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 15.1)Penicillinase_R; Penicillinase repressor (PF03965; HMM-score: 13.8)PaaX; PaaX-like protein (PF07848; HMM-score: 13.1)ISOCOT_Fold (CL0246) CoA_trans; Coenzyme A transferase (PF01144; HMM-score: 12.6)HTH (CL0123) Rrf2; Transcriptional regulator (PF02082; HMM-score: 12.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effector: Galactose-6-phosphate
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.031751
- TAT(Tat/SPI): 0.000485
- LIPO(Sec/SPII): 0.000351
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVSDMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASLIEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMRHITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYLLIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [2] [3]
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [1] Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e) - ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e)
⊟Relevant publications[edit | edit source]
B Oskouian, G C Stewart
Repression and catabolite repression of the lactose operon of Staphylococcus aureus.
J Bacteriol: 1990, 172(7);3804-12
[PubMed:2163387] [WorldCat.org] [DOI] (P p)