From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02452
  • pan locus tag?: SAUPAN005588000
  • symbol: SAOUHSC_02452
  • pan gene symbol?: lacD
  • synonym:
  • product: tagatose 1,6-diphosphate aldolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02452
  • symbol: SAOUHSC_02452
  • product: tagatose 1,6-diphosphate aldolase
  • replicon: chromosome
  • strand: -
  • coordinates: 2276636..2277616
  • length: 981
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTCAAAATCGAATCAAAAAATCGCATCAATTGAACAATTAAGTAACAACGAAGGTATC
    ATTTCTGCATTAGCATTTGACCAACGTGGTGCTTTAAAACGCATGATGGCAAAACATCAA
    ACTGAAGAACCAACTGTAGCTCAAATTGAACAATTAAAAGTATTAGTTGCGGAAGAATTA
    ACACAATATGCATCTTCAATTTTATTAGATCCTGAATATGGTTTACCAGCTTCAGATGCA
    CGTAACAAAGATTGTGGATTATTACTTGCTTATGAAAAAACTGGATATGACGTAAACGCT
    AAAGGTCGTTTACCTGACTGTTTAGTAGAGTGGTCTGCAAAACGTTTAAAAGAACAAGGC
    GCAAATGCTGTTAAATTCTTACTTTACTATGATGTTGATGATGCTGAAGAAATTAACATT
    CAAAAGAAAGCATACATTGAAAGAATCGGTTCAGAATGTGTTGCAGAAGATATTCCATTC
    TTTTTAGAAGTATTAACATACGATGACAACATTCCTGATAACGGTAGTGTTGAATTCGCA
    AAAGTTAAGCCTAGAAAGGTTAACGAAGCAATGAAATTATTCTCAGAACCACGCTTTAAC
    GTTGATGTATTGAAAGTTGAAGTACCAGTAAACATGAAATATGTAGAAGGCTTTGCTGAA
    GGTGAAGTGGTTTATACAAAAGAGGAAGCGGCACAACACTTTAAAGATCAAGATGCAGCA
    ACTCATTTACCATACATTTACTTAAGTGCTGGTGTTTCTGCGGAATTATTCCAAGAAACT
    TTAAAATTTGCTCACGAAGCTGGTGCTAAATTCAACGGGGTACTTTGTGGCCGTGCTACA
    TGGTCTGGTGCTGTTCAAGTATACATTGAACAAGGTGAAGATGCTGCTCGTGAATGGTTA
    CGTACAACTGGTTTCAAAAATATTGATGACTTAAATAAAGTATTAAAAGATACTGCGACA
    TCTTGGAAACAAAGAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    981

⊟Protein[edit | edit source]

Protein Data Bank: 3MYP

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02452
  • symbol: SAOUHSC_02452
  • description: tagatose 1,6-diphosphate aldolase
  • length: 326
  • theoretical pI: 4.77016
  • theoretical MW: 36595.2
  • GRAVY: -0.389571

⊟Function[edit | edit source]

  • reaction:
    EC 4.1.2.40?  ExPASy
    Tagatose-bisphosphate aldolase D-tagatose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides tagatose 1,6-diphosphate aldolase (TIGR01232; EC 4.1.2.40; HMM-score: 717.7)
  • TheSEED  :
    • Tagatose 1,6-bisphosphate aldolase (EC 4.1.2.40)
    Carbohydrates Di- and oligosaccharides Lactose and Galactose Uptake and Utilization  Tagatose 1,6-bisphosphate aldolase (EC 4.1.2.40)
    and 1 more
    Carbohydrates Monosaccharides D-Tagatose and Galactitol Utilization  Tagatose 1,6-bisphosphate aldolase (EC 4.1.2.40)
  • PFAM:
    TIM_barrel (CL0036) DeoC; DeoC/LacD family aldolase (PF01791; HMM-score: 263.6)
    and 2 more
    DUF2090; Uncharacterized protein conserved in bacteria (DUF2090) (PF09863; HMM-score: 26.8)
    no clan defined TIP41; TIP41-like family (PF04176; HMM-score: 12.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003114
    • TAT(Tat/SPI): 0.000468
    • LIPO(Sec/SPII): 0.000295
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEPTVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKGRLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPFFLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGFAEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLCGRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: LacR* (repression) regulon
    LacR*(TF)important in Lactose utilization; transcription unit transferred from N315 data RegPrecise  [3]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

E L Rosey, B Oskouian, G C Stewart
Lactose metabolism by Staphylococcus aureus: characterization of lacABCD, the structural genes of the tagatose 6-phosphate pathway.
J Bacteriol: 1991, 173(19);5992-8
[PubMed:1655695] [WorldCat.org] [DOI] (P p)E L Rosey, G C Stewart
The nucleotide sequence of the lacC and lacD genes of Staphylococcus aureus.
Nucleic Acids Res: 1989, 17(10);3980
[PubMed:2543958] [WorldCat.org] [DOI] (P p)

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAOUHSC_02452&oldid=98989"