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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1994 [new locus tag: SA_RS11480 ]
  • pan locus tag?: SAUPAN005588000
  • symbol: lacD
  • pan gene symbol?: lacD
  • synonym:
  • product: tagatose 1,6-diphosphate aldolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1994 [new locus tag: SA_RS11480 ]
  • symbol: lacD
  • product: tagatose 1,6-diphosphate aldolase
  • replicon: chromosome
  • strand: -
  • coordinates: 2266138..2267118
  • length: 981
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTCAAAATCTAATCAAAAAATCGCATCAATTGAACAATTAAGTAATAACGAAGGTATC
    ATTTCTGCATTAGCATTTGACCAACGTGGTGCTTTAAAACGAATGATGGCTAAACATCAA
    ACGGAAGAGCCAACTGTCGCTCAAATTGAACAATTAAAAGTATTGGTTGCGGAAGAATTA
    ACACAATATGCGTCTTCAATTTTATTAGATCCAGAATACGGTTTACCAGCATCTGATGCA
    CGTAACAAAGATTGTGGATTATTGCTAGCTTATGAAAAAACTGGTTATGACGTAAATGCT
    AAAGGTCGTTTACCTGACTGTTTAGTAGAGTGGTCTGCAAAACGTTTAAAAGAACAAGGT
    GCAAATGCTGTTAAATTCTTACTTTACTATGATGTTGATGATGCTGAAGAAATTAACATT
    CAAAAGAAAGCATACATTGAAAGAATCGGTTCAGAATGTGTTGCAGAAGACATTCCTTTC
    TTCTTAGAAGTATTAACATACGATGACAACATTCCTGATAACGGTAGTGTTGAATTCGCA
    AAAGTTAAGCCTAGAAAGGTTAACGAAGCAATGAAATTATTCTCAGAACCACGCTTTAAT
    GTTGATGTATTGAAAGTTGAAGTACCAGTAAACATGAAGTATGTAGAAGGCTTTGCTGAA
    GGTGAAGTGGTTTATACAAAAGAGGAAGCGGCACAACACTTTAAAGATCAAGATGCAGCA
    ACTCATTTACCATACATTTACTTAAGTGCTGGTGTTTCTGCGGAATTATTCCAAGAAACT
    TTAAAATTTGCTCACGAAGCTGGTGCTAAATTCAACGGTGTACTTTGTGGCCGTGCTACA
    TGGTCTGGTGCTGTTCAAGTATACATTGAACAAGGTGAAGATGCTGCTCGTGAATGGTTA
    CGTACAACTGGTTTCAAAAATATTGATGACTTAAATAAAGTATTAAAAGATACTGCGACA
    TCTTGGAAACAAAGAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    981

⊟Protein[edit | edit source]

Protein Data Bank: 3MYP

⊟General[edit | edit source]

  • locus tag: SA1994 [new locus tag: SA_RS11480 ]
  • symbol: LacD
  • description: tagatose 1,6-diphosphate aldolase
  • length: 326
  • theoretical pI: 4.77016
  • theoretical MW: 36595.2
  • GRAVY: -0.389571

⊟Function[edit | edit source]

  • reaction:
    EC 4.1.2.40?  ExPASy
    Tagatose-bisphosphate aldolase D-tagatose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides tagatose 1,6-diphosphate aldolase (TIGR01232; EC 4.1.2.40; HMM-score: 717.7)
  • TheSEED  :
    • Tagatose 1,6-bisphosphate aldolase (EC 4.1.2.40)
    Carbohydrates Di- and oligosaccharides Lactose and Galactose Uptake and Utilization  Tagatose 1,6-bisphosphate aldolase (EC 4.1.2.40)
    and 1 more
    Carbohydrates Monosaccharides D-Tagatose and Galactitol Utilization  Tagatose 1,6-bisphosphate aldolase (EC 4.1.2.40)
  • PFAM:
    TIM_barrel (CL0036) DeoC; DeoC/LacD family aldolase (PF01791; HMM-score: 263.6)
    and 2 more
    DUF2090; Uncharacterized protein conserved in bacteria (DUF2090) (PF09863; HMM-score: 26.8)
    no clan defined TIP41; TIP41-like family (PF04176; HMM-score: 12.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003114
    • TAT(Tat/SPI): 0.000468
    • LIPO(Sec/SPII): 0.000295
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEPTVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKGRLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPFFLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGFAEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLCGRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: LacR (repression) regulon
    LacR(TF)important in Lactose utilization; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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