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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_2096 [new locus tag: NWMN_RS12130 ]
- pan locus tag?: SAUPAN005588000
- symbol: lacD
- pan gene symbol?: lacD
- synonym:
- product: tagatose 1,6-diphosphate aldolase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_2096 [new locus tag: NWMN_RS12130 ]
- symbol: lacD
- product: tagatose 1,6-diphosphate aldolase
- replicon: chromosome
- strand: -
- coordinates: 2334367..2335347
- length: 981
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5332071 NCBI
- RefSeq: YP_001333130 NCBI
- BioCyc:
- MicrobesOnline: 3707698 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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961ATGTCAAAATCGAATCAAAAAATCGCATCAATTGAACAATTAAGTAACAACGAAGGTATC
ATTTCTGCATTAGCATTTGACCAACGTGGTGCTTTAAAACGCATGATGGCAAAACATCAA
ACTGAAGAACCAACTGTAGCTCAAATTGAACAATTAAAAGTATTAGTTGCGGAAGAATTA
ACACAATATGCATCTTCAATTTTATTAGATCCTGAATATGGTTTACCAGCTTCAGATGCA
CGTAACAAAGATTGTGGATTATTACTTGCTTATGAAAAAACTGGATATGACGTAAACGCT
AAAGGTCGTTTACCTGACTGTTTAGTAGAGTGGTCTGCAAAACGTTTAAAAGAACAAGGC
GCAAATGCTGTTAAATTCTTACTTTACTATGATGTTGATGATGCTGAAGAAATTAACATT
CAAAAGAAAGCATACATTGAAAGAATCGGTTCAGAATGTGTTGCAGAAGATATTCCATTC
TTTTTAGAAGTATTAACATACGATGACAACATTCCTGATAACGGTAGTGTTGAATTCGCA
AAAGTTAAGCCTAGAAAGGTTAACGAAGCAATGAAATTATTCTCAGAACCACGCTTTAAC
GTTGATGTATTGAAAGTTGAAGTACCAGTAAACATGAAATATGTAGAAGGCTTTGCTGAA
GGTGAAGTGGTTTATACAAAAGAGGAAGCGGCACAACACTTTAAAGATCAAGATGCAGCA
ACTCATTTACCATACATTTACTTAAGTGCTGGTGTTTCTGCGGAATTATTCCAAGAAACT
TTAAAATTTGCTCACGAAGCTGGTGCTAAATTCAACGGGGTACTTTGTGGCCGTGCTACA
TGGTCTGGTGCTGTTCAAGTATACATTGAACAAGGTGAAGATGCTGCTCGTGAATGGTTA
CGTACAACTGGTTTCAAAAATATTGATGACTTAAATAAAGTATTAAAAGATACTGCGACA
TCTTGGAAACAAAGAAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_2096 [new locus tag: NWMN_RS12130 ]
- symbol: LacD
- description: tagatose 1,6-diphosphate aldolase
- length: 326
- theoretical pI: 4.77016
- theoretical MW: 36595.2
- GRAVY: -0.389571
⊟Function[edit | edit source]
- reaction: EC 4.1.2.40? ExPASyTagatose-bisphosphate aldolase D-tagatose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
- TIGRFAM: Energy metabolism Biosynthesis and degradation of polysaccharides tagatose 1,6-diphosphate aldolase (TIGR01232; EC 4.1.2.40; HMM-score: 717.7)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: TIM_barrel (CL0036) DeoC; DeoC/LacD family aldolase (PF01791; HMM-score: 263.6)and 2 moreDUF2090; Uncharacterized protein conserved in bacteria (DUF2090) (PF09863; HMM-score: 26.8)no clan defined TIP41; TIP41-like family (PF04176; HMM-score: 12.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.003114
- TAT(Tat/SPI): 0.000468
- LIPO(Sec/SPII): 0.000295
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEPTVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKGRLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPFFLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGFAEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLCGRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Regulation[edit | edit source]
- data available for N315
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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