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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1992 [new locus tag: SA_RS11470 ]
  • pan locus tag?: SAUPAN005586000
  • symbol: lacE
  • pan gene symbol?: lacE
  • synonym:
  • product: PTS system lactose-specific transporter subunit IIBC

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1992 [new locus tag: SA_RS11470 ]
  • symbol: lacE
  • product: PTS system lactose-specific transporter subunit IIBC
  • replicon: chromosome
  • strand: -
  • coordinates: 2264087..2265799
  • length: 1713
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATGCAAAAATTGATCGCACAAATTGAAAAAGGGAAGCCATTTTTCGAGAAATTATCA
    CGTAACATATATTTAAGAGCCATCCGTGATGGATTTATTTCTGCAATGCCTGTCATCTTA
    TTCTCAAGTATCTTTCTATTAATTGCATACGTGCCAAATATATTTGGTTTTAAATGGGAT
    AAAGGCATGGAAGCCATTTTAATGAAACCATATAACTATACGATGGGGCTTGTAGCATTT
    TTAGTAGCAGGAACGACAGCGAAATCTTTAACAGATTCATTTAACCGTAAGCTAGAAAGT
    ACAAATCAAATTAACTTTATTTCTACAATGCTAGCAGCGATGTGTGGATTCTTGTTCTTA
    GCTTCTGATCCAGCCAAAGATGGTGGATTCTTAAGTGCATTTATGGGAACTAAAGGTTTA
    TTAACAGCATTTTTATCAGCATTTGTAACTGTTATTGTTTATAACTTCTGTGTGAAGCGC
    AATATTACAATTAAAATGCCGAAAGAAGTACCGCCGAATATTTCACAAGTATTTAAGGAC
    TTAATTCCATTTTCAGCGGTAATCATCATTCTTTATGCATTAGATTTAGTCATTCGCAAC
    AGCTTTAAATCAAATGTAGCGGAAGGTATTTTAAAATTATTCGAACCATTATTTACAGCA
    GCAGATGGATGGATTGGTGTCACAATTATCTTTGGTGCCTTTGCATTATTCTGGTTTGTA
    GGAATTCATGGTCCATCAATAGTAGAGCCAGCAATTGCAGCCATTACATATGCGAATATC
    GAAGCGAACTTCAAGTTGCTTCAAGCTGGAGAACACGCAGATAAAATTATTACATCAGGT
    ACACAAATGTTTATCGTTACTTTTGGAGGTACGGGTGCAACATTGGTCGTCCCGTTCATG
    TTTATGTGGATGACGAAATCTAAACGAAACAAAGCGATTGGACGTGCATCAGTTGTACCA
    ACATTCTTTGGTGTAAACGAACCAATTTTATTTGGTGCACCACTAGTATTGAATCCTGTG
    TTCTTTATTCCATTTGTATTAGCACCAATTGTTAACGTATGGATTTTCAAACTTTTCGTT
    GAAGTGTTAGGAATAAATAGTTTTAGTGTAAATTTACCTTGGACAACACCAGGTCCATTG
    GGCATTATCATGGGTACAGGTTTTGGTTTATGGTCATTCGTACTAGCTATTACTTTGATT
    GTTGTAGATATTATTATTTACTACCCATTCCTAAAAGTTTATGATAGTGAAATTCTTGAT
    GAAGAAGAAGGACGTAAAGAAAGTAATTCAGATTTAAAAGAAAAAGTTGCAGCAAACTTT
    GATACGAAAAAAGCTGATTCAATTTTAGCGGCAAGTGGTGTATCAGACGATGCAGCTAAA
    GCTTCAAATATCACAGAACAAACAAATGTCTTAGTACTTTGTGCCGGTGGTGGTACAAGT
    GGATTATTAGCCAACGCACTTAATAAAGCAGCTGAAGAATATCATGTGCCAGTGAAAGCA
    GCAGCTGGCGGATACGGCGCACATATGGACATTATGAAAGAGTACCAATTAATCATTTTA
    GCGCCACAAGTTGCGTCTAACTATGAAGATATTAAGCAAGATACAGATAGATTAGGTATT
    AAATTAGCGAAGACTCAAGGTGCTGAATATATCAAATTGACACGAGACGGCCAAGCAGCC
    CTTGATTTCGTTCAACAACAATTTGAAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1713

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1992 [new locus tag: SA_RS11470 ]
  • symbol: LacE
  • description: PTS system lactose-specific transporter subunit IIBC
  • length: 570
  • theoretical pI: 7.48602
  • theoretical MW: 62413.8
  • GRAVY: 0.447018

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.207?  ExPASy
    Protein-N(pi)-phosphohistidine--lactose phosphotransferase [Protein]-N(pi)-phospho-L-histidine + lactose(Side 1) = [protein]-L-histidine + lactose 6'-phosphate(Side 2)
  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, lactose-specific IIC component (TIGR00394; EC 2.7.1.69; HMM-score: 769.3)
    Signal transduction Signal transduction PTS PTS system, lactose-specific IIC component (TIGR00394; EC 2.7.1.69; HMM-score: 769.3)
    and 8 more
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, cellobiose-specific IIC component (TIGR00359; EC 2.7.1.69; HMM-score: 268.4)
    Signal transduction Signal transduction PTS PTS system, cellobiose-specific IIC component (TIGR00359; EC 2.7.1.69; HMM-score: 268.4)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, lactose/cellobiose family IIC component (TIGR00410; EC 2.7.1.69; HMM-score: 268.4)
    Signal transduction Signal transduction PTS PTS system, lactose/cellobiose family IIC component (TIGR00410; EC 2.7.1.69; HMM-score: 268.4)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 205.3)
    Signal transduction Signal transduction PTS PTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 205.3)
    PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 34.7)
    PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 26.2)
  • TheSEED  :
    • PTS system, lactose-specific IIB component (EC 2.7.1.69)
    • PTS system, lactose-specific IIC component (EC 2.7.1.69)
    Carbohydrates Di- and oligosaccharides Lactose and Galactose Uptake and Utilization  PTS system, lactose-specific IIB component (EC 2.7.1.69)
    and 1 more
    Carbohydrates Di- and oligosaccharides Lactose and Galactose Uptake and Utilization  PTS system, lactose-specific IIC component (EC 2.7.1.69)
  • PFAM:
    PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 253)
    and 2 more
    Phosphatase (CL0031) PTS_IIB; PTS system, Lactose/Cellobiose specific IIB subunit (PF02302; HMM-score: 40.7)
    PFK (CL0240) DAGK_cat; Diacylglycerol kinase catalytic domain (PF00781; HMM-score: 11.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 0
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001663
    • TAT(Tat/SPI): 0.000132
    • LIPO(Sec/SPII): 0.00121
  • predicted transmembrane helices (TMHMM): 10

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSIFLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTNQINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKRNITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLFTAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKIITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAPLVLNPVFFIPFVLAPIVNVWIFKLFVEVLGINSFSVNLPWTTPGPLGIIMGTGFGLWSFVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILAASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAHMDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQQFEN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: LacR (repression) regulon
    LacR(TF)important in Lactose utilization; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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