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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1997
  • pan locus tag?: SAUPAN005592000
  • symbol: lacA
  • pan gene symbol?: lacA
  • synonym:
  • product: galactose-6-phosphate isomerase subunit LacA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1997
  • symbol: lacA
  • product: galactose-6-phosphate isomerase subunit LacA
  • replicon: chromosome
  • strand: -
  • coordinates: 2268598..2269026
  • length: 429
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGGCGATTATTATTGGTTCAGATGAAGCTGGCAAACGATTAAAAGAAGTCATCAAATCA
    TACTTATTAGACAATAAATATGATGTTGTTGACGTAACAGAAGGACAGGAAGTTGACTTT
    GTTGATGCAACTTTGGCTGTAGCAAAAGATGTTCAAAGTCAAGAAGGTAACTTAGGTATT
    GTTATCGATGCATTTGGTGCAGGTAGCTTTATGGTTGCTACAAAAATCAAAGGCATGATT
    GCTGCTGAAGTATCAGATGAACGCTCTGGATATATGACTAGAGGCCATAACAATTCAAGA
    ATGATCACTATGGGATCTGAAATTGTTGGAGACACATTAGCGAAAAATGTCGTTAAAGGT
    TTTGTTGAAGGTAAATACGATGGTGGTAGACACCAAATCCGCGTAGATATGCTTAATAAA
    ATGTGCTAA
    60
    120
    180
    240
    300
    360
    420
    429

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1997
  • symbol: LacA
  • description: galactose-6-phosphate isomerase subunit LacA
  • length: 142
  • theoretical pI: 4.96862
  • theoretical MW: 15394.5
  • GRAVY: -0.105634

⊟Function[edit | edit source]

  • reaction:
    EC 5.3.1.26?  ExPASy
    Galactose-6-phosphate isomerase D-galactose 6-phosphate = D-tagatose 6-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides galactose-6-phosphate isomerase, LacA subunit (TIGR01118; EC 5.3.1.26; HMM-score: 284.6)
    and 4 more
    sugar-phosphate isomerase, RpiB/LacA/LacB family (TIGR00689; EC 5.3.1.-; HMM-score: 110.3)
    Metabolism Energy metabolism Pentose phosphate pathway ribose 5-phosphate isomerase B (TIGR01120; EC 5.3.1.6; HMM-score: 102.4)
    ribose 5-phosphate isomerase (TIGR02133; EC 5.3.1.6; HMM-score: 79.9)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides galactose-6-phosphate isomerase, LacB subunit (TIGR01119; EC 5.3.1.26; HMM-score: 40.4)
  • TheSEED  :
    • Galactose-6-phosphate isomerase, LacA subunit (EC 5.3.1.26)
    Carbohydrates Di- and oligosaccharides Lactose and Galactose Uptake and Utilization  Galactose-6-phosphate isomerase, LacA subunit (EC 5.3.1.26)
  • PFAM:
    no clan defined LacAB_rpiB; Ribose/Galactose Isomerase (PF02502; HMM-score: 130.1)
    and 1 more
    P-loop_NTPase (CL0023) AFG1_ATPase; AFG1-like ATPase (PF03969; HMM-score: 10.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002486
    • TAT(Tat/SPI): 0.000093
    • LIPO(Sec/SPII): 0.000229
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDATLAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMITMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: LacR (repression) regulon
    LacR(TF)important in Lactose utilization; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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