PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1995 [new locus tag: SA_RS11485 ]
• pan locus tag^?: SAUPAN005589000
• symbol: lacC
• pan gene symbol^?: lacC
• synonym:
• product: tagatose-6-phosphate kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1995 [new locus tag: SA_RS11485 ]
• symbol: lacC
• product: tagatose-6-phosphate kinase
• replicon: chromosome
• strand: -
• coordinates: 2267122..2268054
• length: 933
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124911 NCBI
• RefSeq: NP_375306 NCBI
• BioCyc: see SA_RS11485
• MicrobesOnline: 104332 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGATTTTAACTTTAACATTAAATCCATCCGTTGATATTTCATATCCATTAACAGCATTG
  AAATTAGATGATGTTAATCGTGTTCAAGAAGTAAGTAAAACAGCTGGTGGTAAAGGTTTA
  AATGTAACAAGAGTACTTGCGCAAGTAGGCGAGCCAGTCCTTGCGAGTGGTTTTATTGGC
  GGTGAATTAGGTCAATTTATTGCTAAAAAATTAGATCATGCCGACATCAAGCATGCATTT
  TATAACATTAAGGGTGAAACACGAAACTGCATTGCAATTTTACATGAAGGGCAACAAACA
  GAAATTTTAGAGCAGGGACCGGAGATTGATAATCAAGAGGCTGCAGGATTTATTAAACAT
  TTTGAACAATTATTAGAAAAAGTTGAAGCAGTTGCTATTTCAGGTAGCTTACCTAAAGGA
  TTAAATCAAGATTATTATGCACAAATTATTGAACGATGCCAAAACAAAGGTGTGCCTGTG
  ATATTGGACTGCTCGGGTGCAACTTTGCAAACAGTTTTGGAAAATCCATACAAACCAACA
  GTCATTAAACCAAACATTTCTGAACTATATCAATTGCTTAACCAGCCACTTGATGAAAGT
  TTAGAAAGTTTGAAACAGGCAGTGTCACAACCCTTATTCGAAGGGATTGAGTGGATTATT
  GTGTCTTTAGGTGCACAAGGTGCATTCGCTAAGCACAATCATACATTCTATAGAGTGAAT
  ATTCCGACAATAAACGTATTAAATCCGGTTGGTTCAGGTGATTCTACAGTTGCTGGAATT
  ACTTCAGCAATTCTCAATCATGAAAACGACCACGATTTACTAAAAAAAGCTAATACTTTA
  GGCATGCTGAATGCGCAAGAAGCACAAACAGGATATGTCAATTTAAATAATTATGATGAA
  TTATTCAATCAAATTGAAGTTTTAGAGGTGTAA
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  933
  

⊟Protein

⊟General

• locus tag: SA1995 [new locus tag: SA_RS11485 ]
• symbol: LacC
• description: tagatose-6-phosphate kinase
• length: 310
• theoretical pI: 4.66537
• theoretical MW: 33894.3
• GRAVY: -0.106452

⊟Function

• reaction:
  EC 2.7.1.144?  ExPASy
  Tagatose-6-phosphate kinase ATP + D-tagatose 6-phosphate = ADP + D-tagatose 1,6-bisphosphate
• TIGRFAM:
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides tagatose-6-phosphate kinase (TIGR01231; EC 2.7.1.144; HMM-score: 618.3)
  and 7 more
  hexose kinase, 1-phosphofructokinase family (TIGR03168; EC 2.7.1.-; HMM-score: 324.1)
  1-phosphofructokinase (TIGR03828; EC 2.7.1.56; HMM-score: 279.9)
  Metabolism Energy metabolism Sugars ribokinase (TIGR02152; EC 2.7.1.15; HMM-score: 65.7)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides bifunctional protein RfaE, domain I (TIGR02198; EC 2.7.1.-; HMM-score: 60.2)
  Metabolism Energy metabolism Sugars 5-dehydro-2-deoxygluconokinase (TIGR04382; EC 2.7.1.92; HMM-score: 48.5)
  Unknown function General YjeF family C-terminal domain (TIGR00196; HMM-score: 40.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (TIGR00097; EC 2.7.1.49,2.7.4.7; HMM-score: 11.9)
• TheSEED  :
  • Tagatose-6-phosphate kinase (EC 2.7.1.144)
  Carbohydrates Di- and oligosaccharides Lactose and Galactose Uptake and Utilization  Tagatose-6-phosphate kinase (EC 2.7.1.144)
  and 1 more
  Carbohydrates Monosaccharides D-Tagatose and Galactitol Utilization  Tagatose-6-phosphate kinase (EC 2.7.1.144)
• PFAM:
  Ribokinase (CL0118) PfkB; pfkB family carbohydrate kinase (PF00294; HMM-score: 218.6)
  and 2 more
  Carb_kinase; Carbohydrate kinase (PF01256; HMM-score: 38.6)
  Phos_pyr_kin; Phosphomethylpyrimidine kinase (PF08543; HMM-score: 20.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.013235
  • TAT(Tat/SPI): 0.000863
  • LIPO(Sec/SPII): 0.000994
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927773 NCBI
• RefSeq: NP_375306 NCBI
• UniProt: P65699 UniProt

⊟Protein sequence

• MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTRVLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEILEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPVILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEWIIVSLGAQGAFAKHNHTFYRVNIPTINVLNPVGSGDSTVAGITSAILNHENDHDLLKKANTLGMLNAQEAQTGYVNLNNYDELFNQIEVLEV

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: lacG < lacE < lacF < lacD < lacC < lacB < lacA

⊟Regulation

• regulator: LacR (repression) regulon

  LacR

  (TF)

  important in Lactose utilization; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications