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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02453
  • pan locus tag?: SAUPAN005589000
  • symbol: SAOUHSC_02453
  • pan gene symbol?: lacC
  • synonym:
  • product: tagatose-6-phosphate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02453
  • symbol: SAOUHSC_02453
  • product: tagatose-6-phosphate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 2277620..2278552
  • length: 933
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGATTTTAACTTTAACATTAAATCCATCCGTTGATATTTCATACCCATTAACAGCATTG
    AAATTAGATGATGTTAATCGTGTTCAAGAAGTAAGTAAAACAGCTGGTGGTAAAGGTTTA
    AATGTAACAAGAGTACTTGCGCAAGTAGGTGAGCCAGTCCTTGCGAGTGGTTTTATTGGC
    GGTGAATTAGGTCAATTTATTGCTAAAAAATTAGATCATGCCGACATCAAGCATGCATTT
    TATAACATTAAGGGTGAAACACGAAACTGCATTGCAATTTTACATGAAGGGCAACAAACA
    GAAATTTTAGAGCAGGGACCGGAGATTGATAATCAAGAGGCTGCAGGATTTATTAAACAT
    TTTGAACAATTATTAGAAAAAGTTGAAGCAGTTGCTATTTCAGGTAGCTTACCTAAAGGA
    TTAAATCAAGATTATTATGCACAAATCATTGAACGATGCCAAAACAAAGGTGTGCCTGTG
    ATATTAGATTGCTCGGGTGCAACTTTGCAAACAGTTTTGGAAAATCCATACAAACCTACA
    GTCATTAAACCAAACATTTCTGAACTATATCAATTGCTTAACCAGCCACTTGATGAAAGT
    TTAGAAAGTTTGAAACAGGCAGTGTCACAACCCTTATTCGAAGGGATTGAGTGGATTATT
    GTGTCTTTAGGTGCACAAGGTGCATTCGCTAAGCACAATCATACATTCTATAGAGTGAAT
    ATTCCGACAATAAGTGTATTAAATCCGGTTGGTTCAGGTGATTCTACAGTTGCTGGAATT
    ACTTCAGCAATTCTTAATCATGAAAACGACCACGATTTACTAAAAAAAGCCAATACTTTA
    GGCATGCTGAATGCACAAGAAGCACAAACAGGTTATGTCAATTTAAATAATTATGATGAT
    TTATTCAATCAAATTGAAGTTTTAGAGGTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    933

⊟Protein[edit | edit source]

Protein Data Bank: 2Q5R
Protein Data Bank: 2JG1
Protein Data Bank: 2JGV

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02453
  • symbol: SAOUHSC_02453
  • description: tagatose-6-phosphate kinase
  • length: 310
  • theoretical pI: 4.66037
  • theoretical MW: 33853.2
  • GRAVY: -0.0977419

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.144?  ExPASy
    Tagatose-6-phosphate kinase ATP + D-tagatose 6-phosphate = ADP + D-tagatose 1,6-bisphosphate
  • TIGRFAM:
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides tagatose-6-phosphate kinase (TIGR01231; EC 2.7.1.144; HMM-score: 620.2)
    and 6 more
    hexose kinase, 1-phosphofructokinase family (TIGR03168; EC 2.7.1.-; HMM-score: 323.1)
    1-phosphofructokinase (TIGR03828; EC 2.7.1.56; HMM-score: 278.5)
    Metabolism Energy metabolism Sugars ribokinase (TIGR02152; EC 2.7.1.15; HMM-score: 64.5)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides bifunctional protein RfaE, domain I (TIGR02198; EC 2.7.1.-; HMM-score: 59.3)
    Metabolism Energy metabolism Sugars 5-dehydro-2-deoxygluconokinase (TIGR04382; EC 2.7.1.92; HMM-score: 47.7)
    Unknown function General YjeF family C-terminal domain (TIGR00196; HMM-score: 41.5)
  • TheSEED  :
    • Tagatose-6-phosphate kinase (EC 2.7.1.144)
    Carbohydrates Di- and oligosaccharides Lactose and Galactose Uptake and Utilization  Tagatose-6-phosphate kinase (EC 2.7.1.144)
    and 1 more
    Carbohydrates Monosaccharides D-Tagatose and Galactitol Utilization  Tagatose-6-phosphate kinase (EC 2.7.1.144)
  • PFAM:
    Ribokinase (CL0118) PfkB; pfkB family carbohydrate kinase (PF00294; HMM-score: 217.5)
    and 2 more
    Carb_kinase; Carbohydrate kinase (PF01256; HMM-score: 40)
    Phos_pyr_kin; Phosphomethylpyrimidine kinase (PF08543; HMM-score: 19.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013235
    • TAT(Tat/SPI): 0.000863
    • LIPO(Sec/SPII): 0.000994
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTRVLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEILEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPVILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEWIIVSLGAQGAFAKHNHTFYRVNIPTISVLNPVGSGDSTVAGITSAILNHENDHDLLKKANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: LacR* (repression) regulon
    LacR*(TF)important in Lactose utilization; transcription unit transferred from N315 data RegPrecise  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

E L Rosey, B Oskouian, G C Stewart
Lactose metabolism by Staphylococcus aureus: characterization of lacABCD, the structural genes of the tagatose 6-phosphate pathway.
J Bacteriol: 1991, 173(19);5992-8
[PubMed:1655695] [WorldCat.org] [DOI] (P p)Linda Miallau, William N Hunter, Sean M McSweeney, Gordon A Leonard
Structures of Staphylococcus aureus D-tagatose-6-phosphate kinase implicate domain motions in specificity and mechanism.
J Biol Chem: 2007, 282(27);19948-57
[PubMed:17459874] [WorldCat.org] [DOI] (P p)E L Rosey, G C Stewart
The nucleotide sequence of the lacC and lacD genes of Staphylococcus aureus.
Nucleic Acids Res: 1989, 17(10);3980
[PubMed:2543958] [WorldCat.org] [DOI] (P p)

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