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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL2199 [new locus tag: SACOL_RS11570 ]
- pan locus tag?: SAUPAN005625000
- symbol: budB
- pan gene symbol?: budB
- synonym:
- product: acetolactate synthase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL2199 [new locus tag: SACOL_RS11570 ]
- symbol: budB
- product: acetolactate synthase
- replicon: chromosome
- strand: -
- coordinates: 2280413..2282077
- length: 1665
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3238195 NCBI
- RefSeq: YP_187010 NCBI
- BioCyc: see SACOL_RS11570
- MicrobesOnline: 913684 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1621ATGACTGATAAAAAGTACACTGCAGCCGATATGGTTATTGATACTTTGAAAAATAATGGG
GTAGAATATGTTTTTGGTATTCCGGGTGCAAAGATTGACTATCTATTTAATGCTTTAATT
GATGATGGTCCTGAACTTATTGTCACTCGTCATGAACAAAACGCCGCAATGATGGCGCAA
GGTATTGGAAGATTAACAGGCAAACCGGGTGTAGTACTTGTTACAAGTGGTCCTGGTGTA
AGTAATTTAACTACTGGATTATTAACCGCAACATCTGAAGGGGATCCTGTATTAGCGTTA
GGTGGCCAAGTGAAACGTAATGATTTATTACGATTAACGCATCAAAGTATTGATAATGCT
GCGCTATTAAAATATTCATCAAAATATAGTGAAGAAGTACAAGATCCTGAATCATTATCA
GAAGTTATGACAAATGCAATTCGAATTGCTACTTCAGGAAAAAATGGCGCAAGTTTTATT
AGTATTCCGCAAGACGTTATTTCTTCACCAGTTGAATCTAAAGCTATATCACTTTGCCAA
AAACCAAATTTAGGAGTACCGAGTGAACAAGATATTAATGATGTCATTGAAGCGATTAAA
AATGCATCATTTCCTGTTTTATTAGCTGGTATGAGAAGTTCAAGTGCAGAAGAAACAAAT
GCCATTCGCAAATTAGTTGAGCGCACGAATTTACCAGTTGTAGAAACATTCCAAGGTGCA
GGTGTAATTAGTCGTGAATTAGAAAATCATTTCTTCGGTCGTGTGGGCTTATTCCGCAAT
CAAGTTGGTGATGAATTATTACGTAAAAGTGATTTAGTTGTTACAATCGGTTATGATCCA
ATTGAATACGAAGCTAGTAACTGGAATAAAGAATTAGAAACACAAATTATCAATATTGAC
GAAGTTCAAGCTGAAATTACTAATTATATGCAACCGAAAAAAGAGTTGATTGGTAATATT
GCTAAAACGATTGAAATGATTTCTGAAAAAGTGGATGAGCCATTTATAAATCAACAACAT
TTAGACGAATTAGAACAATTAAGAACACATATTGATGAAGAAACTGGTATTAAAGCGACG
CATGAAGAAGGAATTCTACATCCAGTGGAAATTATTGAATCTATGCAAAAGGTATTAACT
GATGATACTACTGTAACAGTTGATGTTGGAAGTCACTATATTTGGATGGCACGTAATTTC
AGAAGTTACAATCCAAGACATTTATTATTTAGCAATGGTATGCAAACGCTTGGTGTAGCA
TTACCGTGGGCAATTTCAGCTGCACTTGTGCGCCCTAATACGCAAGTTGTGTCCGTTGCT
GGCGATGGTGGCTTTTTATTTTCATCACAAGATTTAGAAACGGCCGTACGTAAAAATTTA
AATATCATCCAGCTTATTTGGAATGATGGAAAATATAACATGGTTGAATTCCAAGAAGAA
ATGAAATATAAACGTTCGTCAGGTGTAGACTTCGGTCCTGTAGATTTTGTAAAATATGCA
GAATCATTTGGCGCGAAAGGTTTACGAGTTACTAATCAAGAAGAATTAGAAGCGGCAATT
AAAGAGGGCTATGAAACAGATGGTCCAGTATTAATTGATATACCTGTAAATTACAAAGAT
AATATCAAACTTTCAACAAATATGTTACCTGACGTATTTAACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL2199 [new locus tag: SACOL_RS11570 ]
- symbol: BudB
- description: acetolactate synthase
- length: 554
- theoretical pI: 4.51625
- theoretical MW: 61168.8
- GRAVY: -0.222383
⊟Function[edit | edit source]
- reaction: EC 2.2.1.6? ExPASyAcetolactate synthase 2 pyruvate = 2-acetolactate + CO2
- TIGRFAM: Energy metabolism Fermentation acetolactate synthase, catabolic (TIGR02418; EC 2.2.1.6; HMM-score: 931.6)and 15 moreAmino acid biosynthesis Pyruvate family acetolactate synthase, large subunit, biosynthetic type (TIGR00118; EC 2.2.1.6; HMM-score: 346.1)Energy metabolism Aerobic pyruvate oxidase (TIGR02720; EC 1.2.3.3; HMM-score: 241.1)Cellular processes Detoxification oxalyl-CoA decarboxylase (TIGR03254; EC 4.1.1.8; HMM-score: 197.2)Central intermediary metabolism Other sulfoacetaldehyde acetyltransferase (TIGR03457; EC 2.3.3.15; HMM-score: 187.4)glyoxylate carboligase (TIGR01504; EC 4.1.1.47; HMM-score: 169.4)Energy metabolism Sugars 3,5/4-trihydroxycyclohexa-1,2-dione hydrolase (TIGR04377; EC 3.7.1.-; HMM-score: 161.9)indolepyruvate/phenylpyruvate decarboxylase (TIGR03394; EC 4.1.1.43,4.1.1.74; HMM-score: 93.6)Central intermediary metabolism Other indolepyruvate decarboxylase (TIGR03393; EC 4.1.1.74; HMM-score: 62.3)Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase (TIGR00173; EC 2.2.1.9; HMM-score: 57.6)phosphonopyruvate decarboxylase (TIGR03297; EC 4.1.1.82; HMM-score: 25.8)sulfopyruvate decarboxylase, beta subunit (TIGR03846; EC 4.1.1.79; HMM-score: 25.1)Biosynthesis of cofactors, prosthetic groups, and carriers Other sulfopyruvate decarboxylase, alpha subunit (TIGR03845; EC 4.1.1.79; HMM-score: 21.2)Energy metabolism Methanogenesis sulfopyruvate decarboxylase, alpha subunit (TIGR03845; EC 4.1.1.79; HMM-score: 21.2)2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family (TIGR02177; HMM-score: 19.3)Energy metabolism Chemoautotrophy CO dehydrogenase/acetyl-CoA synthase complex, epsilon subunit (TIGR00315; EC 1.2.99.2; HMM-score: 17.2)
- TheSEED :
- Acetolactate synthase, catabolic (EC 2.2.1.6)
Carbohydrates Fermentation Acetoin, butanediol metabolism Acetolactate synthase, catabolic (EC 2.2.1.6)and 2 more - PFAM: THDP-binding (CL0254) TPP_enzyme_N; Thiamine pyrophosphate enzyme, N-terminal TPP binding domain (PF02776; HMM-score: 176)TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 150)and 4 moreFAD_DHS (CL0085) TPP_enzyme_M; Thiamine pyrophosphate enzyme, central domain (PF00205; HMM-score: 88.8)P-loop_NTPase (CL0023) AAA_23; AAA domain (PF13476; HMM-score: 17.6)FAD_DHS (CL0085) CO_dh; CO dehydrogenase beta subunit/acetyl-CoA synthase epsilon subunit (PF02552; HMM-score: 14.6)Fusion_gly (CL0595) Baculo_F; Baculovirus F protein (PF12259; HMM-score: 10.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 1.05
- Cytoplasmic Membrane Score: 8.78
- Cellwall Score: 0.08
- Extracellular Score: 0.09
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004002
- TAT(Tat/SPI): 0.000185
- LIPO(Sec/SPII): 0.000309
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGPELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGGQVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFISIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEETNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTIGYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISEKVDEPFINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSHYIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQDLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGLRVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)