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NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_02468
- pan locus tag?: SAUPAN005625000
- symbol: SAOUHSC_02468
- pan gene symbol?: budB
- synonym:
- product: acetolactate synthase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_02468
- symbol: SAOUHSC_02468
- product: acetolactate synthase
- replicon: chromosome
- strand: -
- coordinates: 2291064..2292728
- length: 1665
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3919031 NCBI
- RefSeq: YP_500936 NCBI
- BioCyc: G1I0R-2333 BioCyc
- MicrobesOnline: 1290907 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1621ATGACTGATAAAAAGTACACTGCAGCCGATATGGTTATTGATACTTTGAAAAATAATGGG
GTAGAATATGTTTTTGGTATTCCGGGTGCAAAGATTGACTATCTATTTAATGCTTTAATT
GATGATGGTCCTGAACTTATTGTCACTCGTCATGAACAAAACGCCGCAATGATGGCGCAA
GGTATTGGAAGATTAACAGGCAAACCGGGTGTAGTACTTGTTACAAGTGGTCCTGGTGTA
AGTAATTTAACTACTGGATTATTAACCGCAACATCTGAAGGGGATCCTGTATTAGCGTTA
GGTGGCCAAGTGAAACGTAATGATTTATTACGATTAACGCATCAAAGTATTGATAATGCT
GCGCTATTAAAATATTCATCAAAATATAGTGAAGAAGTACAAGATCCTGAATCATTATCA
GAAGTTATGACAAATGCAATTCGAATTGCTACTTCAGGAAAAAATGGCGCAAGTTTTATT
AGTATTCCGCAAGACGTTATTTCTTCACCAGTTGAATCTAAAGCTATATCACTTTGCCAA
AAAACAAATTTAGGAGTACCGAGTGAACAAGATATTAATGATGTCATTGAAGCGATTAAA
AATGCATCATTTCCTGTTTTATTAGCTGGTATGAGAAGTTCAAGTGCAGAAGAAACAAAT
GCCATTCGCAAATTAGTTGAGCGCACGAATTTACCAGTTGTAGAAACATTCCAAGGTGCA
GGTGTAATTAGTCGTGAATTAGAAAATCATTTCTTCGGTCGTGTGGGCTTATTCCGCAAT
CAAGTTGGTGATGAATTATTACGTAAAAGTGATTTAGTTGTTACAATCGGTTATGATCCA
ATTGAATACGAAGCTAGTAACTGGAATAAAGAATTAGAAACACAAATTATCAATATTGAC
GAAGTTCAAGCTGAAATTACTAATTATATGCAACCGAAAAAAGAGTTGATTGGTAATATT
GCTAAAACGATTGAAATGATTTCTGAAAAAGTGGATGAGCCATTTATAAATCAACAACAT
TTAGACGAATTAGAACAATTAAGAACACATATTGATGAAGAAACTGGTATTAAAGCGACG
CATGAAGAAGGAATTCTACATCCAGTGGAAATTATTGAATCTATGCAAAAGGTATTAACT
GATGATACTACTGTAACAGTTGATGTTGGAAGTCACTATATTTGGATGGCACGTAATTTC
AGAAGTTACAATCCAAGACATTTATTATTTAGCAATGGTATGCAAACGCTTGGTGTAGCA
TTACCGTGGGCAATTTCAGCTGCACTTGTGCGCCCTAATACGCAAGTTGTGTCCGTTGCT
GGCGATGGTGGCTTTTTATTTTCATCACAAGATTTAGAAACGGCCGTACGTAAAAATTTA
AATATCATCCAGCTTATTTGGAATGATGGAAAATATAACATGGTTGAATTCCAAGAAGAA
ATGAAATATAAACGTTCGTCAGGTGTAGACTTCGGTCCTGTAGATTTTGTAAAATATGCA
GAATCATTTGGCGCGAAAGGTTTACGAGTTACTAATCAAGAAGAATTAGAAGCGGCAATT
AAAGAGGGCTATGAAACAGATGGTCCAGTATTAATTGATATACCTGTAAATTACAAAGAT
AATATCAAACTTTCAACAAATATGTTACCTGACGTATTTAACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_02468
- symbol: SAOUHSC_02468
- description: acetolactate synthase
- length: 554
- theoretical pI: 4.51625
- theoretical MW: 61172.8
- GRAVY: -0.220758
⊟Function[edit | edit source]
- reaction: EC 2.2.1.6? ExPASyAcetolactate synthase 2 pyruvate = 2-acetolactate + CO2
- TIGRFAM: Energy metabolism Fermentation acetolactate synthase, catabolic (TIGR02418; EC 2.2.1.6; HMM-score: 929.1)and 15 moreAmino acid biosynthesis Pyruvate family acetolactate synthase, large subunit, biosynthetic type (TIGR00118; EC 2.2.1.6; HMM-score: 346.3)Energy metabolism Aerobic pyruvate oxidase (TIGR02720; EC 1.2.3.3; HMM-score: 241.7)Cellular processes Detoxification oxalyl-CoA decarboxylase (TIGR03254; EC 4.1.1.8; HMM-score: 197.8)Central intermediary metabolism Other sulfoacetaldehyde acetyltransferase (TIGR03457; EC 2.3.3.15; HMM-score: 188.7)glyoxylate carboligase (TIGR01504; EC 4.1.1.47; HMM-score: 168.9)Energy metabolism Sugars 3,5/4-trihydroxycyclohexa-1,2-dione hydrolase (TIGR04377; EC 3.7.1.-; HMM-score: 162.5)indolepyruvate/phenylpyruvate decarboxylase (TIGR03394; EC 4.1.1.43,4.1.1.74; HMM-score: 95.4)Central intermediary metabolism Other indolepyruvate decarboxylase (TIGR03393; EC 4.1.1.74; HMM-score: 62.6)Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase (TIGR00173; EC 2.2.1.9; HMM-score: 58.5)phosphonopyruvate decarboxylase (TIGR03297; EC 4.1.1.82; HMM-score: 25.8)sulfopyruvate decarboxylase, beta subunit (TIGR03846; EC 4.1.1.79; HMM-score: 25.2)Biosynthesis of cofactors, prosthetic groups, and carriers Other sulfopyruvate decarboxylase, alpha subunit (TIGR03845; EC 4.1.1.79; HMM-score: 21.2)Energy metabolism Methanogenesis sulfopyruvate decarboxylase, alpha subunit (TIGR03845; EC 4.1.1.79; HMM-score: 21.2)2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family (TIGR02177; HMM-score: 19.3)Energy metabolism Chemoautotrophy CO dehydrogenase/acetyl-CoA synthase complex, epsilon subunit (TIGR00315; EC 1.2.99.2; HMM-score: 17.2)
- TheSEED :
- Acetolactate synthase, catabolic (EC 2.2.1.6)
Carbohydrates Fermentation Acetoin, butanediol metabolism Acetolactate synthase, catabolic (EC 2.2.1.6)and 2 more - PFAM: THDP-binding (CL0254) TPP_enzyme_N; Thiamine pyrophosphate enzyme, N-terminal TPP binding domain (PF02776; HMM-score: 176)TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 150)and 4 moreFAD_DHS (CL0085) TPP_enzyme_M; Thiamine pyrophosphate enzyme, central domain (PF00205; HMM-score: 88.8)CO_dh; CO dehydrogenase beta subunit/acetyl-CoA synthase epsilon subunit (PF02552; HMM-score: 15.1)P-loop_NTPase (CL0023) AAA_23; AAA domain (PF13476; HMM-score: 13.2)Fusion_gly (CL0595) Baculo_F; Baculovirus F protein (PF12259; HMM-score: 10.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 1.05
- Cytoplasmic Membrane Score: 8.78
- Cellwall Score: 0.08
- Extracellular Score: 0.09
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004002
- TAT(Tat/SPI): 0.000185
- LIPO(Sec/SPII): 0.000309
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGPELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGGQVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFISIPQDVISSPVESKAISLCQKTNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEETNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTIGYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISEKVDEPFINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSHYIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQDLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGLRVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [1] [2]
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [3] Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)