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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2209 [new locus tag: SACOL_RS11630 ]
  • pan locus tag?: SAUPAN005670000
  • symbol: SACOL2209
  • pan gene symbol?: ecfT
  • synonym:
  • product: cobalt transport family protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2209 [new locus tag: SACOL_RS11630 ]
  • symbol: SACOL2209
  • product: cobalt transport family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2290571..2291377
  • length: 807
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAAAATAAATTGATTATAGGGCGATATTTACCAATTAATTCTTTTGTTCATCATCTT
    GATCCAAGAGCAAAGTTAATGTTTGTTTTTCTTTTTATTATATTAATATTTTTCTGTCAT
    TCACCATTAACATATCTTTGGGTGTTTGCACTTATCTTATTCATTATGAGATTAGCAAAA
    ATTCAATTATGGTTCCTAATCAAAGGTTTAACACCAATATTTTTCTTTTTAATCTTCACA
    TTAATGATGCATATATTTTTAACTAAAGGTGGATATGTATTAGTTGAATGGCATGGCATC
    ACGATTGAAACTAATGGTATTTTAGAAGGGCTATATATATCACTGCGTTTAATTGGGATT
    GTAATGATTGCAACAATTATGACACTATCTACAAGTCCAATTGATTTAACAGATGCGTTT
    GAAAGATTACTTGCACCACTAAAGATGTTTAAATTACCAGTTCATCAATTAAGTATGATA
    ATGTCCATTGCGTTACGATTCATCCCGACGTTAATGGATGAGTTAGATAAAATCATTTTG
    GCGCAAAAGTCGCGTGGTTCTGAAATAAGTTCAGGGAACATTGCAACACGTATTAAATCA
    TTTATTCCATTACTAGTGCCACTATTCATCTCAGCTTTCCAACGCGCCGAAGAATTAGCG
    GTCGCAATGGAAGTTAGGGGTTATGATGCCAATGTTAAGAGAACGAGCTACAGACAGCTT
    AAATGGCAATTGAGAGATACGATATCTTTAACCATGATTATCCCAATTGCAATTATTTTA
    TTCGTATTAAAATATTCAGGAGTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    807

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL2209 [new locus tag: SACOL_RS11630 ]
  • symbol: SACOL2209
  • description: cobalt transport family protein
  • length: 268
  • theoretical pI: 10.4263
  • theoretical MW: 30829.3
  • GRAVY: 0.794776

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds cobalt ECF transporter T component CbiQ (TIGR02454; HMM-score: 104)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Biosynthesis  Transmembrane component of general energizing module of ECF transporters
    and 2 more
    Test - Folate  Transmembrane component of general energizing module of ECF transporters
    Membrane Transport Membrane Transport - no subcategory ECF class transporters  Transmembrane component of general energizing module of ECF transporters
  • PFAM:
    no clan defined CbiQ; Cobalt transport protein (PF02361; HMM-score: 164.9)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 5
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001663
    • TAT(Tat/SPI): 0.000088
    • LIPO(Sec/SPII): 0.029493
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLTYLWVFALILFIMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITIETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMIMSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEELAVAMEVRGYDANVKRTSYRQLKWQLRDTISLTMIIPIAIILFVLKYSGV

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
    experimental localization: Integral membrane [1] [2]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]