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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2302 [new locus tag: SACOL_RS12110 ]
  • pan locus tag?: SAUPAN005788000
  • symbol: SACOL2302
  • pan gene symbol?:
  • synonym:
  • product: transcriptional regulator

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2302 [new locus tag: SACOL_RS12110 ]
  • symbol: SACOL2302
  • product: transcriptional regulator
  • replicon: chromosome
  • strand: -
  • coordinates: 2364682..2365635
  • length: 954
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    GTGGGATTGAAACGTTCGTCTAAAAGTAAAATGAGCCTACCGAAAAAAATATTTTTATGG
    GTATTCGGTATTTTAGTAATCTTAGCGATTGTAGCTGTTGTGTATGTAGCTGCTAAAATT
    TTTATTACTGGTAATAAGATACACAATCCGTTAGATCGCAATCATTCAGAATTAAGAGAT
    AAAAAAGTGAGTTTAAACGATGGTGATCCATTCACAATCGCTTTATTTGGTGTCGATTCA
    GATGCTGATCGTAAGAAAAAGGGTGGCGGAGAGCGTAGTGATAGTATTATGATTTTATCT
    ATCAACCCTAAAACGAAGAAAACTGAAATAGTAAGTATACCGCGTGATACAAGAGCGGAA
    ATTGTTGGCCGTGGTACTACTGAAAAAATTGCACATGCCTATGCATATGGTGGACCAAAT
    ATGGCTGTTAAATCACTTGAAAAATTAATGAATGTGCCAATTGATCATTATGCGACAATC
    GATATGGATGGTTTGCATAATATGATAGATAGCATCGGCGGTGTTGATGTAGTAAGTAAT
    GATACATTCACTGTTGATGGTGTTCGTTTTACAAAAGGACAGCAGACACATGTAAATGGT
    GATCAAGCATTGAAGTTTATTAGAAGTCGTAAAGAAGAAGGTGCTGGTGGTGACTTTGGT
    CGACAACAACGTCAGCAAATTGTGCTTGAAGCGATGGCCAATAAGATTGCAAGCCCATCA
    TCGATTACACATTTTAATAGTCTAATGAATGAAATTCAAAATAATGTTAAAACAGATTTA
    ACATTAGGTGATTTGAATACGATTAGAAGCAATTATAAAGATGCTAATGACACGATTAAT
    AAACATCAATTGAGTGGTCAAGGCGGCATTCAAAGTGACGGATTGTACTACTTCATTCCT
    AGTGAGCAGTCAAAAGCTGAAAGTACTAAGTTATTAAAAGATAATCTAGAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    954

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL2302 [new locus tag: SACOL_RS12110 ]
  • symbol: SACOL2302
  • description: transcriptional regulator
  • length: 317
  • theoretical pI: 9.70036
  • theoretical MW: 34907.5
  • GRAVY: -0.390536

Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions Other cell envelope-related function transcriptional attenuator common domain (TIGR00350; HMM-score: 149.3)
  • TheSEED:  
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Cell envelope-associated LytR-CpsA-Psr transcriptional attenuators  Cell envelope-associated transcriptional attenuator LytR-CpsA-Psr, subfamily F2 (as in PMID19099556)
  • PFAM:
    no clan defined LytR_cpsA_psr; Cell envelope-related transcriptional attenuator domain (PF03816; HMM-score: 166.8)
    and 1 more
    DUF3053; Protein of unknown function (DUF3053) (PF11254; HMM-score: 12.6)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 7
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.155798
    • TAT(Tat/SPI): 0.001432
    • LIPO(Sec/SPII): 0.011887
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MGLKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNKIHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPKTKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDMDGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFGRQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDTINKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE

Experimental data[edit | edit source]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

  • SACOL2302 no polycistronic organisation predicted

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]