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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2259 [new locus tag: SAUSA300_RS12480 ]
  • pan locus tag?: SAUPAN005788000
  • symbol: SAUSA300_2259
  • pan gene symbol?: —
  • synonym:
  • product: putative transcriptional regulator

lcpC: capsular peptidoglycan: teichoic acid ligase C

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2259 [new locus tag: SAUSA300_RS12480 ]
  • symbol: SAUSA300_2259
  • product: putative transcriptional regulator
  • replicon: chromosome
  • strand: -
  • coordinates: 2428549..2429472
  • length: 924
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGCCTACCGAAAAAAATATTTTTATGGGTATTCGGTATTTTAGTAATCTTAGCGATT
    GTAGCTGTTGTGTATGTAGCTGCTAAAATTTTTATTACTGGTAATAAGATACACAATCCG
    TTAGATCGCAATCATTCAGAATTAAGAGATAAAAAAGTGAGTTTAAACGATGGTGATCCA
    TTCACAATCGCTTTATTTGGTGTCGATTCAGATGCTGATCGTAAGAAAAAGGGTGGCGGA
    GAGCGTAGTGATAGTATTATGATTTTATCTATCAACCCTAAAACGAAGAAAACTGAAATA
    GTAAGTATACCGCGTGATACAAGAGCGGAAATTGTTGGCCGTGGTACTACTGAAAAAATT
    GCACATGCCTATGCATATGGTGGACCAAATATGGCTGTTAAATCACTTGAAAAATTAATG
    AATGTGCCAATTGATCATTATGCGACAATCGATATGGATGGTTTGCATAATATGATAGAT
    AGCATCGGCGGTGTTGATGTAGTAAGTAATGATACATTCACTGTTGATGGTGTTCGTTTT
    ACAAAAGGACAGCAGACACATGTAAATGGTGATCAAGCATTGAAGTTTATTAGAAGTCGT
    AAAGAAGAAGGTGCTGGTGGTGACTTTGGTCGACAACAACGTCAGCAAATTGTGCTTGAA
    GCGATGGCCAATAAGATTGCAAGCCCATCATCGATTACACATTTTAATAGTCTAATGAAT
    GAAATTCAAAATAATGTTAAAACAGATTTAACATTAGGTGATTTGAATACGATTAGAAGC
    AATTATAAAGATGCTAATGACACGATTAATAAACATCAATTGAGTGGTCAAGGCGGCATT
    CAAAGTGACGGATTGTACTACTTCATTCCTAGTGAGCAGTCAAAAGCTGAAAGTACTAAG
    TTATTAAAAGATAATCTAGAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    924

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2259 [new locus tag: SAUSA300_RS12480 ]
  • symbol: SAUSA300_2259
  • description: putative transcriptional regulator
  • length: 307
  • theoretical pI: 8.71513
  • theoretical MW: 33804.2
  • GRAVY: -0.359935

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions Other cell envelope-related function transcriptional attenuator common domain (TIGR00350; HMM-score: 149.4)
  • TheSEED  :
    • Cell envelope-associated transcriptional attenuator LytR-CpsA-Psr, subfamily F2
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Cell envelope-associated LytR-CpsA-Psr transcriptional attenuators  Cell envelope-associated transcriptional attenuator LytR-CpsA-Psr, subfamily F2 (as in PMID19099556)
  • PFAM:
    no clan defined LytR_cpsA_psr; Cell envelope-related transcriptional attenuator domain (PF03816; HMM-score: 166.9)
    and 1 more
    DUF3053; Protein of unknown function (DUF3053) (PF11254; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 7
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.180738
    • TAT(Tat/SPI): 0.002035
    • LIPO(Sec/SPII): 0.018103
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNKIHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPKTKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDMDGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFGRQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDTINKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Yvonne Gar-Yun Chan, Hwan Keun Kim, Olaf Schneewind, Dominique Missiakas
    The capsular polysaccharide of Staphylococcus aureus is attached to peptidoglycan by the LytR-CpsA-Psr (LCP) family of enzymes.
    J Biol Chem: 2014, 289(22);15680-90
    [PubMed:24753256] [WorldCat.org] [DOI] (I p)    Kaitlin Schaefer, Leigh M Matano, Yuan Qiao, Daniel Kahne, Suzanne Walker
    In vitro reconstitution demonstrates the cell wall ligase activity of LCP proteins.
    Nat Chem Biol: 2017, 13(4);396-401
    [PubMed:28166208] [WorldCat.org] [DOI] (I p)
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